Patent US5468606A successfully refreshed from Google Patents.
US5468606A
Expired / Lapsed
US
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Devices for detection of an analyte based upon light interference
Assignee: Inverness Medical Biostar Inc
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Filing: 1992-07-31
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Granted: 1995-11-21
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Expires: 2012-07-31
Abstract
Abstract Device for detecting the presence or amount of an analyte of interest, having a substrate possessing an optically active surface which exhibits a first color in response to light impinging thereon, and exhibits a second color comprising a combination of wavelengths of light different from the first color or comprising an intensity of at least one wavelength of light different from the first color, in response to the light when the analyte is present on the surface in any amount selected from 0.1 nM, 0.1 ng/ml, 50 fg, and 2x103 organisms comprising the analyte.
Inventors
- 👤 Gregory R. Bogart
- 👤 Garret R. Moddel
- 👤 Diana M. Maul
- 👤 Jeffrey B. Etter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 19:20
CPC Classifications
B; B82; B82Y; B82Y30/00; B82Y15/00; G; G01; G01N; G01N21/00; G01N21/17; G01N21/21; G01N21/211; G01N21/47; G01N21/4738; G01N21/75; G01N21/77; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/551; G01N33/552; G01N21/41; G01N21/45; Y; Y10; Y10S; Y10S435/00; Y10S435/808; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 49
Claim 1
Independent Claim
110 words
An optical assay device for detecting the presence or amount of an analyte of interest comprising: a substrate consisting of one or more layers having an optically active surface exhibiting a first color in response to light impinging thereon, and exhibiting a second color comprising a combination of wavelengths of light different from said first color in response to said light when said analyte is present on said surface, an anti-reflective film provided on said substrate, an attachment layer selected from the group consisting of a polymeric silane, polymeric siloxane, and a dendrimer, on said anti-reflective film and, a specific binding layer for said analyte, attached to said attachment layer.
Claim 2
Dependent Claim
110 words
An optical assay device for detecting the presence or amount of an analyte of interest comprising: a substrate consisting of one or more layers having an optically active surface exhibiting a first color in response to light impinging thereon, and exhibiting a second color comprising a combination of wavelengths of light different from said first color in response to said light when said analyte is present on said surface, an anti-reflective film provided on said substrate, an attachment layer selected from the group consisting of a polymeric silane, polymeric siloxane, and a dendrimer, on said anti-reflective film and, a specific binding layer for said analyte, attached to said attachment layer.
Claim 3
Dependent Claim
94 words
The device of claim 1, wherein said substrate comprises glass; an aluminum layer of between 1900 and 2100 Å thickness is provided on said glass; an amorphous silicon layer having a thickness between 900 and 1100 nm, is provided on said aluminum layer; said anti-reflective film lies on top of said amorphous silicon layer and has a thickness between 480 and 520 Å; said attachment layer is an aminoalkyl-T-structured branched siloxane of between 90 and 110 Å thickness; and said specific binding layer is an antibody layer of between 30 and 60 Å thickness.
Claim 4
Dependent Claim
68 words
The device of claim 1, wherein said substrate comprises monocrystalline silicon; the anti-reflective film is selected from the group consisting of silicon nitride, composites of silicon/silicon dioxide, titanates and titanium dioxide and is is 480-520 Å thick; the attachment layer is aminoalkyl-T-structured branched siloxane and is between 90 and 110 Å thick; and the specific binding layer is an antibody and is between 30 and 60 Å thick.
Claim 5
Dependent Claim
98 words
The device of claim 1, wherein said substrate is glass; and further comprises an amorphous silicon layer between 900 and 1100 nm thick is provided on said glass; the anti-reflective film lies on top of said amorphous silicon layer and is composed of a material selected from the group consisting of silicon nitride, composites of silicon/silicon dioxide, titanates, and titanium dioxide and is 480-520 Å thick; the attachment layer is an aminoalkyl-T-structured branched siloxane and is between 90 and 110 Å thick; and the specific binding layer is an antibody and is between 30 and 60 Å thick.
Claim 6
Dependent Claim
98 words
The device of claim 1, wherein said substrate is plastic; and further comprises an amorphous silicon layer between 900 and 1100 nm thick is provided on said plastic; the anti-reflective film lies on top of said amorphous silicon layer and is composed of a material selected from the group consisting of silicon nitride, composites of silicon/silicon dioxide, titanates, and titanium dioxide and is 480-520 Å thick; the attachment layer is an aminoalkyl-T-structured branched siloxane and is between 90 and 110 Å thick; and the specific binding layer is an antibody and is between 30 and 60 Å thick.
Claim 7
Dependent Claim
110 words
The device of claim 1, wherein said substrate is plastic; and further comprises an aluminum layer between 1800 and 2200 Å thickness is provided on said plastic an amorphous silicon layer has a thickness between 900 and 1100 nm, and is provided on said aluminum layer; the anti-reflective film is selected from the group consisting of silicon nitride, composites of silicon/silicon dioxide, titanates, and titanium dioxide and is 480-520 Å thick and is provided on said amorphous silicon layer; the attachment layer is aminoalkyl-T-structured branched siloxane and is between 90 and 110 Å thick; and the specific binding layer is an antibody and is between 30 and 60 Å thick.
Claim 8
Dependent Claim
37 words
The device of claim 1, wherein said anti-reflective film is composed of a material selected from the group consisting of silicon nitride, composites of silicon/silicon dioxide, titanates, silicon carbide, diamond, cadmium sulfide, titanium dioxide, and silicon oxynitride.
Claim 9
Dependent Claim
51 words
The device of claim 1 for detecting the presence or amount of an analyte of interest, wherein said anti-reflective film, located below the attachment layer, comprises a material selected from the group consisting of silicon nitride, silicon/silicon dioxide composites, silicon oxynitride, titanium dioxide, titanates, diamond, oxides of zirconium, and silicon carbide.
Claim 10
Dependent Claim
83 words
The device of claim 1 for detecting the presence or amount of an analyte of interest, wherein said substrate is a material selected from the group consisting of monocrystalline silicon, glass, a glass/amorphous silicon composite, a plastic, a plastic/amorphous silicon composite, a metal, a ceramic, polycrystalline silicon, and composites of these materials; and wherein said anti-reflective film comprises a material selected from the group consisting of silicon nitride, silicon/silicon dioxide composites, silicon oxynitride, titanium dioxide, titanates, diamond, oxides of zirconium, and silicon carbide.
Claim 11
Independent Claim
27 words
The device of any of claims 8 or 9, wherein said anti-reflective film is coated on said device in a thickness between 480 Å and 520 Å.
Claim 12
Dependent Claim
26 words
The device of claim 1, wherein said first color is golden in appearance and said second color is purple or blue in appearance to the eye.
Claim 13
Dependent Claim
24 words
The device of claim 1, wherein said device is configured and arranged to provide a symbol detectable by eye in response to polychromatic light.
Claim 14
Dependent Claim
105 words
An optical assay device for detecting the presence or amount of an analyte of interest comprising: a substrate consisting of one or more layers having an optically active surface exhibiting a first color in response to light impinging thereon, and exhibiting a second color comprising an intensity of at least one wavelength of light different from said first color, in response to said light when said analyte is present on said surface; an attachment layer selected from the group consisting of a polymeric silane, polymeric siloxane, and a dendrimer, on said substrate and; a specific binding layer for said analyte, attached to said attachment layer.
Claim 15
Dependent Claim
105 words
An optical assay device for detecting the presence or amount of an analyte of interest comprising: a substrate consisting of one or more layers having an optically active surface exhibiting a first color in response to light impinging thereon, and exhibiting a second color comprising an intensity of at least one wavelength of light different from said first color, in response to said light when said analyte is present on said surface; an attachment layer selected from the group consisting of a polymeric silane, polymeric siloxane, and a dendrimer, on said substrate and; a specific binding layer for said analyte, attached to said attachment layer.
Claim 16
Dependent Claim
48 words
The device of claim 1 or 13 for detecting the presence or amount of an analyte of interest, wherein said analyte is present on said surface in an amount selected from the group consisting of 0.1 nM, 0.1 ng/ml, 50 fg, and 2×10 3 organisms comprising said analyte.
Claim 17
Dependent Claim
49 words
The device of claim 1 or 13 for detecting the presence or amount of an analyte of interest, wherein said substrate is a material selected from the group consisting of monocrystalline silicon, a glass/amorphous silicon composite, a plastic/amorphous silicon composite, a ceramic, polycrystalline silicon, and composites of these materials.
Claim 18
Independent Claim
28 words
The device of any of claims 1 or 13, wherein said second color is discernable in less than one hour after contact of said analyte with said device.
Claim 19
Independent Claim
16 words
The device of any of claims 1 or 13, wherein said optically active surface is specular.
Claim 20
Independent Claim
34 words
The device of any of claims 1 or 13, wherein said substrate has a non-specular surface or wherein a transparent layer having a non-specular surface is provided for viewing of said optically active surface.
Claim 21
Dependent Claim
12 words
The device of claim 18, wherein said substrate has a non-specular surface.
Claim 22
Dependent Claim
14 words
The device of claim 18, wherein said substrate is coated with a non-specular material.
Claim 23
Dependent Claim
30 words
The device of claim 18, wherein said substrate is selected from the group consisting of a solid support, a flexible support, a plastic, a glass, a metal and a non-metal,
Claim 24
Dependent Claim
14 words
The device of claim 18, wherein said substrate is light reflective or light transmissive.
Claim 25
Dependent Claim
18 words
The device of claim 18, wherein said light is monochromatic light, polychromatic light, ultraviolet light, or infrared light.
Claim 26
Dependent Claim
76 words
The device of claim 18, wherein said analyte is selected from the group consisting of rheumatoid factor, IgE antibodies specific for Birch pollen, carcinoembryonic antigen, Streptococcus Group A antigen, viral antigens, antigens associated with autoimmune disease, allergens, a tumor or an infectious microorganism, Streptococcus Group B antigen, HIV I or HIV II antigen or host response to said virus, antigens specific to Respiratory Syncytial virus or host response to said virus, and antigens specific to Hepatitis.
Claim 27
Dependent Claim
51 words
The device of claim 18, wherein said non-specular surface has a reading of between 2700 and 3295 with a profilometer, wherein said value represents the RMS roughness divided by the average peak of the textures, and whose specular reflectances measured by a HeNe laser light source is less than about 5%.
Claim 28
Dependent Claim
35 words
The device of claim 18, wherein said substrate is selected from the group consisting of glass, and plastic, and comprising a layer of amorphous silicon on its surface, whereby an optically active surface is produced.
Claim 29
Dependent Claim
36 words
The device of claim 18, wherein said specific binding layer is formed from material selected from the group consisting of antigens, antibodies, oligonucleotides, chelators, enzymes, nucleic acids, polysaccharides, lipids, carbohydrates, metals, and receptors for said materials.
Claim 30
Dependent Claim
26 words
The device of claim 18, wherein said first color is golden in appearance and said second color is purple or blue in appearance to the eye.
Claim 31
Dependent Claim
33 words
The device of claim 18, wherein said specific binding layer is formed from material selected from the group consisting of bacteria, bacterial pill, bacterial flagellar materials, viruses, hormones and receptors for said materials.
Claim 32
Independent Claim
22 words
The device of any of claims 1 or 13, wherein said light is monochromatic light, polychromatic light, ultraviolet light, or infrared light.
Claim 33
Independent Claim
80 words
The device of any of claims 1 or 13, wherein said analyte is selected from the group consisting of rheumatoid factor, IgE antibodies specific for Birch pollen, carcinoembryonic antigen, Streptococcus Group A antigen, viral antigens, antigens associated with autoimmune disease, allergens, a tumor or an infectious microorganism, Streptococcus Group B antigen, HIV I or HIV II antigen or host response to said virus, antigens specific to Respiratory Syncytial virus or host response to said virus, and antigens specific to Hepatitis.
Claim 34
Independent Claim
39 words
The device of any of claims 1 or 13, wherein said substrate is selected from the group consisting of glass, and plastic, and comprising a layer of amorphous silicon on its surface, whereby an optically active surface is produced.
Claim 35
Independent Claim
38 words
The device of claims 1 or 13, wherein said specific binding layer is formed from material selected form the group consisting of antigens, antibodies, oligonucleotides, chelators, enzymes, nucleic acids, polysaccharides, lipids, carbohydrates, metals, and receptors for said materials.
Claim 36
Independent Claim
32 words
The device of any of claims 1 or 13, wherein said analyte is selected from the group consisting of an antibody, antigen, enzyme, hormone, polysaccharides, proteins, lipids, carbohydrates, drugs and nucleic acid.
Claim 37
Independent Claim
35 words
The device of any of claims 1 or 13, wherein said substrate is selected from the group consisting of monocrystalline silicon, glass, fused silica, plastics, metals and semiconductor materials, and may be rigid or flexible.
Claim 38
Dependent Claim
61 words
The device of claim 1 or 13 wherein said analyte is selected from the group consisting of an antigen derived from a bacterium, an antigen derived form a virus, a microorganism, a hapten, a drug of abuse, a therapeutic drug, dioxane, PCB's, heavy metals, total petroleum hydrocarbons, chlorinated hydrocarbons, petroleum byproducts, pentachlorophenol, PNA's, an antibody, an enzyme, and a nucleic acid.
Claim 39
Independent Claim
26 words
The device of any of claims 1 or 13, wherein said analyte of interest is sandwiched between said specific binding layer and a secondary binding reagent.
Claim 40
Independent Claim
22 words
The device of any of claims 1 or 13, wherein the analyte of interest is the Streptococcus group A or B antigen.
Claim 41
Independent Claim
48 words
The device of any of claims 1 or 13, wherein said analyte of interest is or is derived from the causative organisms for meningitis; Neisseria meningitidis groups A, B, C, Y and W 135 , Streptococcus pneumoniae, E. coli K1, Streptococcus group B, and Haemophilus influenzae type B.
Claim 42
Independent Claim
54 words
The device of any of claims 1 or 13, wherein said substrate comprises: a layer of base material positioned adjacent to and beneath a conducting metal layer comprising aluminum, chromium, or a transparent conducting oxide, and a layer of amorphous silicon, wherein said metal layer is positioned adjacent to and below said amorphous silicon.
Claim 43
Dependent Claim
33 words
The device of claim 40, wherein said base material is selected from any of the group consisting of glass, fused silica, plastics, semiconductors, ceramics, and metals, and may be either rigid or flexible.
Claim 44
Independent Claim
40 words
The device of any of claims 1 or 13, for use in an optical assay for an analyte, wherein said substrate comprises a layer of base material, and a layer of amorphous silicon adjacent and positioned above said base material.
Claim 45
Dependent Claim
33 words
The device of claim 42, wherein said base material is selected from any of the group consisting of glass, fused silica, plastics, semiconductors, ceramics, and metals, and may be either rigid or flexible.
Claim 46
Dependent Claim
36 words
The device of claim 1 or 13, wherein the impinging light is reflected by said device and said reflected light is elliptically or linearly polarized, monochromatic, polychromatic, unpolarized, visible, UV, or IR, or any combination thereof.
Claim 47
Dependent Claim
18 words
The device of claim 13, wherein said change is in the intensity of light transmitted through said surface.
Claim 48
Dependent Claim
26 words
The device of claim 1 or 13, wherein the transmitted light is elliptically or linearly polarized, monochromatic, polychromatic, unpolarized, visible, UV, IR, or any combination thereof.
Claim 49
Dependent Claim
37 words
The devices of any of claims 1 or 13, wherein said specific binding layer is formed from material selected from the group consisting of bacteria, bacterial pili, bacterial flagellar materials, viruses, hormones and receptors for said materials.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 7.5 Years (Year 8) | 2002-11-20 | 2003-05-22 | 2003-11-21 | $3760.00 | Window Closed / Lapsed |
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