Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
40 words
A diffraction immunoassay plate for use in a light diffraction immunoassay comprising a surface selected from the group consisting of polysilicon and crystalline silicon surfaces having on said surface, a biological diffraction grating design of lines of active binding reagent.
Claim 2
Dependent Claim
40 words
A diffraction immunoassay plate for use in a light diffraction immunoassay comprising a surface selected from the group consisting of polysilicon and crystalline silicon surfaces having on said surface, a biological diffraction grating design of lines of active binding reagent.
Claim 3
Dependent Claim
15 words
The diffraction immunoassay plate of claim 1 wherein the diffraction binding assay surface is polysilicon.
Claim 4
Dependent Claim
14 words
The diffraction immunoassay plate of claim 1 wherein the active binding reagent is proteinaceous.
Claim 5
Dependent Claim
27 words
The diffraction immunoassay plate of claim 1 wherein the binding reagent is selected from the group consisting of antibody, antigen, hapten, protein A, lectin, biotin and avidin.
Claim 6
Dependent Claim
22 words
The diffraction immunoassay plate of claim 1 wherein the biological diffraction grating design of lines of active binding reagent is non-light disturbing.
Claim 7
Dependent Claim
116 words
A diffraction binding assay method for determining the presence or quantity of an analyte in an aqueous sample comprising (a) contacting a diffraction binding assay surface with the sample for time sufficient to permit conjugation of binding reagent and analyte, the diffraction binding assay surface being selected from the group consisting of polysilicon and single crystalline silicon surfaces having on said surface, a light disturbing design of substantially non-light disturbing binding reagent thereon, the binding reagent being selected to bind selectively with the analyte; (b) separating the surface from the sample; and (c) illuminating the diffraction binding assay surface with light from a light source, and determining the light diffraction by the diffraction binding assay surface.
Claim 8
Dependent Claim
116 words
A diffraction binding assay method for determining the presence or quantity of an analyte in an aqueous sample comprising (a) contacting a diffraction binding assay surface with the sample for time sufficient to permit conjugation of binding reagent and analyte, the diffraction binding assay surface being selected from the group consisting of polysilicon and single crystalline silicon surfaces having on said surface, a light disturbing design of substantially non-light disturbing binding reagent thereon, the binding reagent being selected to bind selectively with the analyte; (b) separating the surface from the sample; and (c) illuminating the diffraction binding assay surface with light from a light source, and determining the light diffraction by the diffraction binding assay surface.
Claim 9
Dependent Claim
16 words
The diffraction binding assay method of claim 6 wherein the diffraction binding assay surface is polysilicon.
Claim 10
Dependent Claim
25 words
The diffraction binding assay method of claim 6 wherein the smooth insoluble surface has been treated with an aqueous solution of a non-specific binding inhibitor.
Claim 11
Dependent Claim
20 words
The diffraction binding assay method of claim 6 wherein the non-specific binding inhibitor is a water-soluble protein or polyamino acid.