Patent US5459058A successfully refreshed from Google Patents.
US5459058A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell culture system
Assignee: Individual
•
Filing: 1994-11-21
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Granted: 1995-10-17
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Expires: 2014-11-21
Abstract
Abstract Disclosed is a method for the culture of higher eukaryotic cells which are dependent for survival on an exogenous factor. The method involves co-culturing the factor-dependent cells with an immortalized eukaryotic cell that has been engineered to secrete the requisite factor. Also disclosed is a cell line of non-stromal cell origin which secretes interleukin-7.
Inventors
- 👤 Philip Leder
- 👤 Benjamin E. Rich
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 16:48
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/5418; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0634; C12N5/0635; C12N5/0636; A; A61; A61K; A61K35/00; A61K35/12; A61K2035/124; C12N2501/00; C12N2501/20; C12N2501/23; C12N2502/00; C12N2502/13; C12N2502/1394; C12N2502/99
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
35 words
A method for culturing a lymphoid cell which is dependent for survival upon an interleukin, said method comprising co-culturing said cell with an immortalized higher eukaryotic cell that has been engineered to secrete said interleukin.
Claim 2
Independent Claim
35 words
A method for culturing a lymphoid cell which is dependent for survival upon an interleukin, said method comprising co-culturing said cell with an immortalized higher eukaryotic cell that has been engineered to secrete said interleukin.
Claim 3
Dependent Claim
23 words
The method of claim 1, wherein said lymphoid cell dependent for survival upon an interleukin is further dependent for survival on cell-cell contact.
Claim 4
Dependent Claim
19 words
The method of claim 1, wherein said lymphoid cell dependent for survival upon an interleukin is a non-adherent cell.
Claim 5
Dependent Claim
19 words
The method of claim 1, wherein said lymphoid cell dependent for survival upon an interleukin is a human cell.
Claim 6
Dependent Claim
13 words
The method of claim 1, wherein said lymphoid cell is a B-lineage lymphocyte.
Claim 7
Dependent Claim
13 words
The method of claim 1, wherein said lymphoid cell is a T-lineage lymphocyte.
Claim 8
Dependent Claim
12 words
The method of claim 1, wherein said lymphoid cell is a thymocyte.
Claim 9
Dependent Claim
15 words
The method of claim 1, wherein said immortalized higher eukaryotic cell is an adherent cell.
Claim 10
Dependent Claim
15 words
The method of claim 1, wherein said immortalized higher eukaryotic cell is a mammalian cell.
Claim 11
Dependent Claim
13 words
The method of claim 9, wherein said mammalian cell is a human cell.
Claim 12
Dependent Claim
15 words
The method of claim 1, wherein said immortalized higher eukaryotic cell is a fibroblast cell.
Claim 13
Dependent Claim
13 words
The method of claim 11, wherein said fibroblast cell is an NIH3T3 cell.
Claim 14
Dependent Claim
10 words
The method of claim 1, wherein said interleukin is IL-7.
Claim 15
Dependent Claim
21 words
The method of claim 13, wherein said IL-7 is expressed from a stably transfected gene in said immortalized higher eukaryotic cell.
Claim 16
Dependent Claim
13 words
The method of claim 14, wherein said stably transfected gene comprises an enhancer.
Claim 17
Dependent Claim
13 words
The method of claim 15, wherein said IL-7 is encoded by plasmid pNAIL-7.
Claim 18
Dependent Claim
10 words
The method of claim 1, wherein said culturing is long-term.
Claim 19
Dependent Claim
35 words
The method of claim 1, further comprising contacting said immortalized higher eukaryotic cell with a growth-inhibitory amount of mitomycin-C just prior to co-culturing with said lymphoid cell which is dependent for survival upon an interleukin.
References Cited (Backward Citations)
US5030105A
Cited
US4965195A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-10-16 | 1999-04-17 | 1999-10-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-10-16 | 2003-04-17 | 2003-10-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-10-16 | 2007-04-17 | 2007-10-17 | $7700.00 | Window Closed / Lapsed |