US4965195A
Expired / Lapsed
US
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Interleukin-7
Assignee: Sanofi Aventis France
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Filing: 1988-10-07
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Granted: 1990-10-23
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Expires: 2008-10-07
Abstract
Abstract Mammalian Interleukin-7 proteins (IL-7s), DNAs and expression vectors encoding mammalian IL-7s, and processes for producing mammalian IL-7s as products of cell culture, including recombinant systems, are disclosed.
Inventors
- 👤 Anthony E. Namen
- 👤 Raymond G. Goodwin
- 👤 Stephen D. Lupton
- 👤 Diane Y. Mochizuki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 19:33
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/5418; C07K16/00; C07K16/18; C07K16/24; C07K16/244; C12N15/11; C12N15/62; C12N15/625; C12N15/70; C12N15/85; A; A61; A61K; A61K38/00; C07K2317/00; C07K2317/30; C07K2317/34; C07K2319/00; C07K2319/01; C07K2319/036; C07K2319/40; C07K2319/43; C07K2319/50; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
47 words
An isolated and purified double-stranded DNA molecule encoding a polypeptide displaying interleukin 7 biological activity, wherein (A) one DNA strand of said molecule hybridizes to a labeled oligonucleotide probe, under hybridization conditions of 50° C. and 0.9 M NaCl, followed by washing in 1×SSC at 55° C.
Claim 2
Dependent Claim
47 words
An isolated and purified double-stranded DNA molecule encoding a polypeptide displaying interleukin 7 biological activity, wherein (A) one DNA strand of said molecule hybridizes to a labeled oligonucleotide probe, under hybridization conditions of 50° C. and 0.9 M NaCl, followed by washing in 1×SSC at 55° C.
Claim 3
Dependent Claim
21 words
An isolated and purified DNA molecule according to claim 1, said molecule encoding a biologically active murine IL-7 or IL-7 mutein.
Claim 4
Dependent Claim
24 words
An isolated and purified DNA molecule according to claim 2, which encodes a polypeptide having the amino-acid sequence of residues 1-129 in FIG. 3.
Claim 5
Dependent Claim
21 words
An isolated and purified DNA molecule according to claim 1, said molecule encoding a biologically active human IL-7 or IL-7 mutein.
Claim 6
Dependent Claim
24 words
An isolated and purified DNA molecule according to claim 4, which encodes a polypeptide having the amino-acid sequence of residues 1-152 in FIG. 5.
Claim 7
Dependent Claim
12 words
A recombinant expression vector comprising a DNA molecule according to claim 1.
Claim 8
Dependent Claim
12 words
A recombinant expression vector comprising a DNA molecule according to claim 4.
Claim 9
Dependent Claim
12 words
A recombinant expression vector comprising a DNA molecule according to claim 5.
Claim 10
Dependent Claim
50 words
A process for preparing a polypeptide comprising a mammalian IL-7, or IL-7 mutein, said polypeptide displaying IL-7 biological activity, comprising the steps of (a) culturing a host cell comprising a vector according to claim 6, such that said polypeptide is expressed in recoverable amounts; and then (b) recovering said polypeptide.
Claim 11
Dependent Claim
50 words
A process for preparing a polypeptide comprising a mammalian IL-7, or IL-7 mutein, said polypeptide displaying IL-7 biological activity, comprising the steps of (a) culturing a host cell comprising a vector according to claim 7, such that said polypeptide is expressed in recoverable amounts; and then (b) recovering said polypeptide.
Claim 12
Dependent Claim
50 words
A process for preparing a polypeptide comprising a mammalian IL-7, or IL-7 mutein, said polypeptide displaying IL-7 biological activity, comprising the steps of (a) culturing a host cell comprising a vector according to claim 8, such that said polypeptide is expressed in recoverable amounts; and then (b) recovering said polypeptide.
Claim 13
Dependent Claim
59 words
A process according to claim 9, wherein said host cell is of the species E. coli and wherein step (b) comprises (1) harvesting said cells, (2) extracting said cells in a denaturing buffer to obtain a cell extract comprising said polypeptide and (3) rapidly diluting with culture medium said cell extract to produce said polypeptide having said biological activity.
Claim 14
Dependent Claim
59 words
A process according to claim 10, wherein said host cell is of the species E. coli and wherein step (b) comprises (1) harvesting said cells, (2) extracting said cells in a denaturing buffer to obtain a cell extract comprising said polypeptide and (3) rapidly diluting with culture medium said cell extract to produce said polypeptide having said biological activity.
Claim 15
Dependent Claim
59 words
A process according to claim 11, wherein said host cell is of the species E. coli and wherein step (b) comprises (1) harvesting said cells, (2) extracting said cells in a denaturing buffer to obtain a cell extract comprising said polypeptide and (3) rapidly diluting with culture medium said cell extract to produce said polypeptide having said biological activity.
Claim 16
Dependent Claim
38 words
A process according to claim 9, wherein said host cell is a BHK or COS-7 cell and wherein step (b) comprises (1) harvesting a culture supernatant of said host cell comprising said polypeptide and (2) recovering said polypeptide.
Claim 17
Dependent Claim
38 words
A process according to claim 10, wherein said host cell is a BHK or COS-7 cell and wherein step (b) comprises (1) harvesting a culture supernatant of said host cell comprising said polypeptide and (2) recovering said polypeptide.
Claim 18
Dependent Claim
38 words
A process according to claim 11, wherein said host cell is a BHK or COS-7 cell and wherein step (b) comprises (1) harvesting a culture supernatant of said host cell comprising said polypeptide and (2) recovering said polypeptide.
References Cited (Backward Citations)
US4675285A
Cited
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