US5030105A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the long-term surviving culture of hepatocytes
Assignee: Center for Research and Advanced Studies of the National Polytechnic Institute
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Filing: 1989-11-15
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Granted: 1991-07-09
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Expires: 2009-11-15
Abstract
Abstract A process is disclosed for the long term surviving culture of hepatocytes maintaining their morphological and functional characteristics for long-term-periods, suitable to assest the acute and chronic hepatotoxicity of drugs, chemicals and environmental pollutants. The long term surviving culture of hepatocytes is carried out in a culture media containing fibroblast cells or fibroblast cell products treated to prevent cell multiplication and inoculated under controlled density.
Inventors
- 👤 Walid Kuri-Harcuch
- 👤 Tomas Mendoza-Figueroa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:38
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/067; C12N2501/00; C12N2501/06; C12N2501/30; C12N2501/38; C12N2501/39; C12N2501/70; C12N2502/00; C12N2502/13; C12N2503/00; C12N2503/02
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
98 words
A method for the assessment of toxicological, chemical, biological and pharmacological agents, comprising: treating long-term surviving cultured hepatocytes with said agents at diverse concentrations, said long-term surviving cultured hepatocytes comprising hepatocyte cells at a density of from about 180 to about 65,000 cells/cm 2 in a culture medium containing from about 1 to about 100 μg/ml of hydrocortisone, in the presence of either multiplication inhibited fibroblast cells or fibroblast cell products on a modified substrate, at controlled densities of from about 30,000 to about 65,000 cells/cm 2 , assessing said hepatocyte cells for injury caused by said agents.
Claim 2
Independent Claim
98 words
A method for the assessment of toxicological, chemical, biological and pharmacological agents, comprising: treating long-term surviving cultured hepatocytes with said agents at diverse concentrations, said long-term surviving cultured hepatocytes comprising hepatocyte cells at a density of from about 180 to about 65,000 cells/cm 2 in a culture medium containing from about 1 to about 100 μg/ml of hydrocortisone, in the presence of either multiplication inhibited fibroblast cells or fibroblast cell products on a modified substrate, at controlled densities of from about 30,000 to about 65,000 cells/cm 2 , assessing said hepatocyte cells for injury caused by said agents.
Claim 3
Dependent Claim
28 words
The method as claimed in claim 1, wherein the treatment of said hepatocytes with said agents produce intermediate metabolites with biological and toxicological action on other organ cells.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-07-08 | 1995-01-07 | 1995-07-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-07-08 | 1999-01-07 | 1999-07-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-07-08 | 2003-01-07 | 2003-07-09 | $7700.00 | Window Closed / Lapsed |