§
Luxuse
Patent Intelligence
Sign In
Patent US5446137A successfully refreshed from Google Patents.
← Back to Patent Explorer
US5446137A Expired / Lapsed US ⏱️ 0.0 years remaining

Oligonucleotides containing 4'-substituted nucleotides

Assignee: Siemens Healthcare Diagnostics GmbH Germany
•
Filing: 1993-12-09
•
Granted: 1995-08-29
•
Expires: 2013-12-09
📄 Original PDF 🌐 Google Patents 🔄 Sync Now

Abstract

Abstract This invention relates to oligonucleotides having at least one nucleotide that is substituted at the 4' position of the sugar moiety with a substituent other than hydrogen. These oligonucleotides are useful in hybridization assays and as therapeutic agents.

Inventors

  • 👤 Hans Maag
  • 👤 Samuel J. Rose
  • 👤 Beat Schmidt

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-29 06:54
CPC Classifications
C; C07; C07H; C07H21/00

Claims Analysis

Total Claims: 19
Claim 1
Independent Claim 136 words
An oligonucleotide which comprises at least one nucleotide having the structure: ##STR15## wherein: A is a purine or a pyrimidine; B and B' are selected from the group consisting of H, OH, F, OCH 3 and SCH 3 , provided that at least one is H; D' is selected from the group consisting of OH, O--P(O)(OH)--OX, O--P(S)(OH)--OX, O--P(S)(SH)--OX and O--P(O)(CH 3 )--OX, where X is selected from the group consisting of H, a nucleotide, and a protecting group; E' is RY, where Y is selected from the group consisting of H and a substituent that renders said nucleotide modifiable, separable or detectable and R is a linking group; and F is selected from the group consisting of OH and O--P(O)(OH)--OX, where X is selected from the group consisting of H, a nucleotide and a protecting group.
Claim 2
Dependent Claim 136 words
An oligonucleotide which comprises at least one nucleotide having the structure: ##STR15## wherein: A is a purine or a pyrimidine; B and B' are selected from the group consisting of H, OH, F, OCH 3 and SCH 3 , provided that at least one is H; D' is selected from the group consisting of OH, O--P(O)(OH)--OX, O--P(S)(OH)--OX, O--P(S)(SH)--OX and O--P(O)(CH 3 )--OX, where X is selected from the group consisting of H, a nucleotide, and a protecting group; E' is RY, where Y is selected from the group consisting of H and a substituent that renders said nucleotide modifiable, separable or detectable and R is a linking group; and F is selected from the group consisting of OH and O--P(O)(OH)--OX, where X is selected from the group consisting of H, a nucleotide and a protecting group.
Claim 3
Dependent Claim 22 words
The oligonucleotide of claim 1 wherein R is selected from the group consisting of alkylene, alkyl, alkenyl, alkadienyl, aralkyl, aminoalkyl and alkylether.
Claim 4
Dependent Claim 35 words
The oligonucleotide of claim 1 wherein said protecting group is selected from the group consisting of phthalyl, carbobenzyloxy, benzyl, benzoyl, trityl, monomethoxytrityl, dimethoxytrityl, acetyl, trifluoroacetyl, trimethylsilyl, t-butyl(dimethyl)silyl, t-butyl(diphenyl) silyl, carbonate, 2-trimethylsilylethyl, methoxymethyl, 2-methoxyethoxymethyl and dihydropyranyl.
Claim 5
Dependent Claim 9 words
The oligonucleotide of claim 1 wherein Y is biotin.
Claim 6
Dependent Claim 35 words
The oligonucleotide of claim 1 wherein Y is a reactive functional group that renders said oligonucleotide modifiable and is selected from the group consisting of NH 2 , SH, OH, COOH, NHC(O)CF 3 and CHO.
Claim 7
Dependent Claim 35 words
The oligonucleotide of claim 1 wherein Y is a substituent that renders said oligonucleotide modifiable and is selected from the group consisting of nucleic acid modifying catalysts, DNA intercalators, minor groove binders and cleavage agents.
Claim 8
Dependent Claim 23 words
The oligonucleotide of claim 6 wherein said nucleic acid modifying catalyst is selected from the group consisting of nucleases, methylases and metal chelates.
Claim 9
Dependent Claim 30 words
The oligonucleotide of claim 6 wherein said DNA intercalator is selected from the group consisting of ethidium, ethidium bromide, acridines, phenazine, phenazinium salts, pyrenes, diazapyrenes, psoralen, psoralen analogs and anthraquinones.
Claim 10
Dependent Claim 21 words
The oligonucleotide of claim 6 wherein said minor groove binder is selected from the group consisting of distamycin, netropsin and bis-benzimidazoles.
Claim 11
Dependent Claim 21 words
The oligonucleotide of claim 6 wherein said cleavage agent is selected from the group consisting of metal complexes and DNA intercalators.
Claim 12
Dependent Claim 21 words
The oligonucleotide of claim 1 wherein Y is selected from the group consisting of detectable labels and specific binding pair members.
Claim 13
Dependent Claim 23 words
The oligonucleotide of claim 11 wherein said detectable label is selected from the group consisting of enzymes, fluorescers, chemiluminescers, sensitizers, particles and catalysts.
Claim 14
Dependent Claim 22 words
The oligonucleotide of claim 1 wherein A is selected from the group consisting of adenine, guanine, xanthine, hypoxanthine, uracil, thymine and cytosine.
Claim 15
Dependent Claim 18 words
The oligonucleotide of claim 1 wherein A is selected from the group consisting of 5-(1-propynyl)pyrimidine, 5-halopyrimidines and 7-deaza-7-alkylpurines.
Claim 16
Independent Claim 46 words
A method of producing an oligonucleotide, which comprises the step of incorporating into said oligonucleotide at least one nucleotide having a substituent other than hydrogen at the 4' position of the sugar moiety, said substituent producing, or being capable of producing, the alteration of said oligonucleotide.
Claim 17
Independent Claim 46 words
A method of producing an oligonucleotide, which comprises the step of incorporating into said oligonucleotide at least one nucleotide having a substituent other than hydrogen at the 4' position of the sugar moiety, said substituent producing, or being capable of producing, the alteration of said oligonucleotide.
Claim 18
Dependent Claim 33 words
The method of claim 15 wherein said incorporating involves the step of synthesizing said oligonucleotide using a nucleotide precursor having a substituent other than hydrogen at the 4' position of the sugar moiety.
Claim 19
Dependent Claim 29 words
The method of claim 15 wherein said altering further comprises the step of hybridizing to said oligonucleotide a polynucleotide that lacks a nucleotide having a 4'-substituent other than hydrogen.

References Cited (Backward Citations)

US4247544A Cited
US4267171A Cited
US4711955A Cited
EP0302175A2 Cited
US4994373A Cited
US4948882A Cited
EP0123300A2 Cited
US4707440A Cited
US4996143A Cited
US5082830A Cited
EP0457326A1 Cited
EP0479640A2 Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1998-08-28 1999-02-27 1999-08-29 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2002-08-28 2003-02-27 2003-08-29 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2006-08-28 2007-02-27 2007-08-29 $7700.00 Window Closed / Lapsed

Assigned Categories & Tags

No categories assigned to this patent yet.

Assign Category Tag