US4948882A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
Assignee: Syngene Inc
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Filing: 1987-05-04
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Granted: 1990-08-14
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Expires: 2007-05-04
Abstract
Abstract Substantially pure single-stranded oligonucleotides having a preselected sequence of not more than about 200 nucleotides, at least one of which is at a preselected position in the sequence and includes a base with a covalently attached linker arm containing or capable of binding at least one reporter group or solid support. A process for the chemical synthesis of the substantially pure single-stranded oligonucleotide and modified nucleosides useful in such synthesis are provided.
Inventors
- 👤 Jerry L. Ruth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 19:10
CPC Classifications
C; C07; C07H; C07H21/00; C07H19/00; C07H19/02; C07H19/04; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
55 words
A compound useful as an intermediate in the chemical synthesis of a single-stranded oligonucleotide comprising the structure: ##STR21## wherein B is a pyrimidine or purine base; R is a linker arm attached to a compound selected from the group consisting of a blocking group, a detectable label and a solid surface; and provided that ##EQU1##
Claim 2
Dependent Claim
55 words
A compound useful as an intermediate in the chemical synthesis of a single-stranded oligonucleotide comprising the structure: ##STR21## wherein B is a pyrimidine or purine base; R is a linker arm attached to a compound selected from the group consisting of a blocking group, a detectable label and a solid surface; and provided that ##EQU1##
Claim 3
Dependent Claim
57 words
The compound of claim 1 wherein R is --CH.sub.2 CHR.sub.1 C.sub.n H.sub.2n Y; --CH═CR.sub.1 C.sub.n H.sub.2n Y; --CHR.sub.1 C.sub.n H.sub.2n Y; ##STR22## Wherein R.sub.1 is H or alkyl, n is 0 to 20 and Y contains a blocked amino or blocked carboxyl or blocked hydroxy or blocked thio group or a reporter group or a solid support.
Claim 4
Dependent Claim
16 words
The modified nucleoside monomer of claim 1 wherein R is attached at a sterically tolerant site.
Claim 5
Dependent Claim
29 words
The modified nucleoside monomer of claim 1 wherein R is attached at the C-5 position when B is a pyrimidine or the C-8 position when B is a purine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-08-13 | 1994-02-12 | 1994-08-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-08-13 | 1998-02-12 | 1998-08-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-08-13 | 2002-02-12 | 2002-08-14 | $7700.00 | Window Closed / Lapsed |