Patent US5429931A successfully refreshed from Google Patents.
US5429931A
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Multilayer analytical element containing crosslinked binder and method for the determination of ethanol
Assignee: Clinical Diagnostic Systems Inc
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Filing: 1993-05-24
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Granted: 1995-07-04
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Expires: 2013-05-24
Abstract
Abstract A multilayer analytical element has been prepared for accurate and rapid colorimetric determination of ethanol in aqueous specimens using alcohol dehydrogenase and an oxidized nicotinamide coenzyme. The element includes at least one reagent layer beneath a porous spreading layer. This reagent layer has a crosslinked polymeric binder prepared, in part, with a polymerizable monomer having a halomethylcarbonyl, haloethylcarbonyl, halomethylsulfonyl or haloethylsulfonyl group. Alcohol dehydrogenase is in a layer of the element for reaction with the analyte.
Inventors
- 👤 Richard L. Detwiler
- 👤 Stephen C. Hasselberg
- 👤 Ignazio S. Ponticello
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 17:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/526; G01N33/483; G01N33/497; G01N33/4972; Y; Y10; Y10T; Y10T436/00; Y10T436/17; Y10T436/20; Y10T436/203332; Y10T436/204165
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
126 words
An analytical element for the determination of ethanol comprising a support having thereon, in order and in fluid contact: a) a first reagent layer containing a buffer having a primary amine, said buffer maintaining the pH at from about 8 to about 10 during an assay for ethanol, and a binder comprising a crosslinked polymer derived by addition polymerization of: i) one or more ethylenically unsaturated polymerizable first monomers selected from the group consisting of an acrylamide and a vinyl pyrrolidone, and ii) an ethylenically unsaturated crosslinkable polymerizable second monomer having a halomethylcarbonyl, haloethylcarbonyl, halomethylsulfonyl or haloethylsulfonyl group, said polymer having been crosslinked with a diamine or dithiol, and b) a porous spreading layer, said element further comprising an alcohol dehydrogenase and an oxidized nicotinamide coenzyme.
Claim 2
Dependent Claim
126 words
An analytical element for the determination of ethanol comprising a support having thereon, in order and in fluid contact: a) a first reagent layer containing a buffer having a primary amine, said buffer maintaining the pH at from about 8 to about 10 during an assay for ethanol, and a binder comprising a crosslinked polymer derived by addition polymerization of: i) one or more ethylenically unsaturated polymerizable first monomers selected from the group consisting of an acrylamide and a vinyl pyrrolidone, and ii) an ethylenically unsaturated crosslinkable polymerizable second monomer having a halomethylcarbonyl, haloethylcarbonyl, halomethylsulfonyl or haloethylsulfonyl group, said polymer having been crosslinked with a diamine or dithiol, and b) a porous spreading layer, said element further comprising an alcohol dehydrogenase and an oxidized nicotinamide coenzyme.
Claim 3
Dependent Claim
15 words
The element of claim 1 wherein said second monomer has a halomethylcarbonyl or haloethylcarbonyl group.
Claim 4
Dependent Claim
41 words
The element of claim 1 wherein said one or more first monomers are selected from the group consisting of vinyl chloroacetate, N-(3-chloroacetamidopropyl)methacrylamide, 2-chloroacetamidoethyl methacrylate, 4chloroacetamidostyrene, p-chloracetamidomethylstyrene, N-(3-chloroacetamidocarbonyliminopropyl)methacrylamide, 2-chloroacetamidocarbonyliminoethyl methacrylate, 4-chloroacetamidocarbonyliminostyrene, m & p-chloroacetamidocarbonyliminomethylstyrene, N-vinyl-N'-(3-chloropropionyl)urea, 4-(3-chloropropionamido)styrene, 4-(3-chloropropionamidocarbonylimino)styrene, 2-(3-chloropropionamido) ethyl methacrylate and N-[2-(3-chloropropionamido)ethyl]methacrylamide.
Claim 5
Dependent Claim
16 words
The element of claim 1 wherein said crosslinked polymer is derived from acrylamide, N-vinyl-2-pyrrolidone and N-(3-chloroacetamidopropyl)methacrylamide.
Claim 6
Dependent Claim
39 words
The element of claim 1 wherein said crosslinked polymer in prepared from about 90 to about 99.8 weight percent of said one or more first monomers, and from about 0.2 to about 10 weight percent of said second monomer.
Claim 7
Dependent Claim
14 words
The element of claim 1 wherein said buffer is tris(hydroxymethyl)aminomethane, tris(methyl)aminomethane or tris(hydroxymethyl)aminomethane glutamate.
Claim 8
Dependent Claim
10 words
The element of claim 6 wherein said buffer is tris(hydroxymethyl)aminomethane.
Claim 9
Dependent Claim
23 words
The element of claim 1 wherein said oxidized nicotinamide coenzyme is in said porous spreading layer which is formed from a blush polymer.
Claim 10
Dependent Claim
24 words
The element of claim 1 wherein said binder in said first reagent layer has been crosslinked with ethylenediamine, 1,3-propanediamine, 1,3-propanediol, dithiothreitol, dithioerythritol or butylenediamine.
Claim 11
Dependent Claim
33 words
The element of claim 1 further comprising a second reagent layer between said first reagent layer and said porous spreading layer, said second reagent layer containing an alcohol dehydrogenase in a hydrophilic binder.
Claim 12
Dependent Claim
16 words
The element of claim 10 wherein said second reagent layer comprises gelatin as said hydrophilic binder.
Claim 13
Dependent Claim
16 words
The element of claim 10 wherein said first and second reagent layers comprise the same binder.
Claim 14
Dependent Claim
13 words
The element of claim 1 wherein said nicotinamide coenzyme is nicotinamide adenine dinucleotide.
Claim 15
Dependent Claim
20 words
The element of claim 10 further comprising a subbing layer between said second reagent layer and said porous spreading layer.
Claim 16
Independent Claim
158 words
A method for the detection of ethanol comprising: A) contacting an aqueous fluid suspected of containing ethanol with an analytical element comprising a support having thereon, in order and in fluid contact: a) a first reagent layer containing a buffer having a primary amine, said buffer maintaining the pH at from about 8 to about 10 during said method, and a binder comprising a crosslinked polymer derived by addition polymerization of: i) one or more ethylenically unsaturated polymerizable monomers selected from the group consisting of acrylamide and a vinyl pyrrolidone, and ii) an ethylenically unsaturated crosslinkable polymerizable monomer having a halomethylcarbonyl, haloethylcarbonyl, halomethylsulfonyl or haloethylsulfonyl group, said polymer having been crosslinked with a diamine or dithiol, and b) a porous spreading layer, said element further comprising alcohol dehydrogenase and an oxidized nicotinamide coenzyme, and B) detecting the absorbence of the reduced form of said nicotinamide coenzyme as an indication of the presence of ethanol in said aqueous fluid.
Claim 17
Independent Claim
158 words
A method for the detection of ethanol comprising: A) contacting an aqueous fluid suspected of containing ethanol with an analytical element comprising a support having thereon, in order and in fluid contact: a) a first reagent layer containing a buffer having a primary amine, said buffer maintaining the pH at from about 8 to about 10 during said method, and a binder comprising a crosslinked polymer derived by addition polymerization of: i) one or more ethylenically unsaturated polymerizable monomers selected from the group consisting of acrylamide and a vinyl pyrrolidone, and ii) an ethylenically unsaturated crosslinkable polymerizable monomer having a halomethylcarbonyl, haloethylcarbonyl, halomethylsulfonyl or haloethylsulfonyl group, said polymer having been crosslinked with a diamine or dithiol, and b) a porous spreading layer, said element further comprising alcohol dehydrogenase and an oxidized nicotinamide coenzyme, and B) detecting the absorbence of the reduced form of said nicotinamide coenzyme as an indication of the presence of ethanol in said aqueous fluid.
Claim 18
Dependent Claim
22 words
The method of claim 15 wherein said detection step B) is carried out within about 5 minutes of said contacting step A).
Claim 19
Dependent Claim
16 words
The method of claim 15 wherein said aqueous fluid is human whole blood, serum or plasma.
Claim 20
Dependent Claim
22 words
The method of claim 15 wherein said ethanol is detected from measuring the absorbence of the reduced form of nicotinamide adenine dinucleotide.
Claim 21
Dependent Claim
21 words
The method of claim 15 wherein said buffer maintains the pH of said element at from about 8.5 to about 9.
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