US5073603A
Expired / Lapsed
US
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Method of using dithiothreitol as a crosslinking agent for electrophoresis media
Assignee: Eastman Kodak Co
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Filing: 1989-11-02
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Granted: 1991-12-17
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Expires: 2009-11-02
Abstract
Abstract Dithiothreitol, a bis-mercaptan, is a surprisingly superior crosslinking agent for preparing electrophoresis media from aqueous solutions of preformed, water-soluble, acrylamide based copolymers comprising a minor proportion of a comonomer, which comonomer contains a site that will react in a crosslinking reaction with a bis-mercaptan.
Inventors
- 👤 Ignazio S. Ponticello
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 17:07
CPC Classifications
C; C08; C08F; C08F220/00; C08F220/02; C08F220/52; C08F220/54; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
134 words
A method of crosslinking a copolymer consisting essentially of a water soluble vinyl addition copolymer derived from a mixture of monomers comprising from 85-98 mole percent a monomer selected from acrylamide and N-substituted acrylamides wherein the N-substituent is an alkyl group having from 1 to 5 carbon atoms, from 2 to 15 mole percent of a vinyl monomer having a haloacetyl reactive group; and from 0 to 12 mole percent of one or more other polymerizable nonionic vinyl monomers selected from styrene monomers, acrylic monomers, methacrylamide monomers and N-substituted acrylamide monomers wherein the substitutent contains at least 6 carbon atoms which method comprises mixing an aqueous solution of said water soluble copolymer with a crosslinking agent that is a bis mercaptan having in its backbone chain the ##STR10## group selected from dithiothreitol or dithioerythritol.
Claim 2
Independent Claim
134 words
A method of crosslinking a copolymer consisting essentially of a water soluble vinyl addition copolymer derived from a mixture of monomers comprising from 85-98 mole percent a monomer selected from acrylamide and N-substituted acrylamides wherein the N-substituent is an alkyl group having from 1 to 5 carbon atoms, from 2 to 15 mole percent of a vinyl monomer having a haloacetyl reactive group; and from 0 to 12 mole percent of one or more other polymerizable nonionic vinyl monomers selected from styrene monomers, acrylic monomers, methacrylamide monomers and N-substituted acrylamide monomers wherein the substitutent contains at least 6 carbon atoms which method comprises mixing an aqueous solution of said water soluble copolymer with a crosslinking agent that is a bis mercaptan having in its backbone chain the ##STR10## group selected from dithiothreitol or dithioerythritol.
Claim 3
Independent Claim
60 words
A method for crosslinking a copolymer comprising a water soluble vinyl addition copolymer derived from a mixture of monomers comprising from 85-98 mole percent a monomer selected from an unsubstituted acrylamide and from 2 to 15 mole percent of a vinyl monomer having chloroacetyl reactive group, which method comprises mixing an aqueous solution of said water soluble copolymer with dithiothreitol.
Claim 4
Independent Claim
60 words
A method for crosslinking a copolymer comprising a water soluble vinyl addition copolymer derived from a mixture of monomers comprising from 85-98 mole percent a monomer selected from an unsubstituted acrylamide and from 2 to 15 mole percent of a vinyl monomer having chloroacetyl reactive group, which method comprises mixing an aqueous solution of said water soluble copolymer with dithiothreitol.
Claim 5
Dependent Claim
29 words
The method of claim 1 wherein the number of equivalents of crosslinking sites per gram of copolymer is in the range of 0.45(10 -4 ) to 14(10 -4 ).
Claim 6
Dependent Claim
36 words
The method of claim 1 wherein said copolymer has a number average molecular weight, M n , of at least about 7,000 and a weight average molecular weight, M w , of less than about 100,000.
Claim 7
Dependent Claim
16 words
The method of claim 4 wherein said M n is from about 7,000 to about 30,000.
Claim 8
Dependent Claim
16 words
The method of claim 4 wherein said M w is from about 25,000 to about 100,000.
Claim 9
Dependent Claim
24 words
The method of claim 1 wherein said copolymer has a M n greater than about 50,000 and a M w greater than about 100,000.
Claim 10
Dependent Claim
14 words
The method of claim 7 wherein said M w is greater than about 150,000.
Claim 11
Dependent Claim
14 words
The method of claim 7 wherein said M w is greater than about 200,000.
Claim 12
Dependent Claim
14 words
The method of claim 7 wherein said M w is greater than about 300,000.
Claim 13
Dependent Claim
24 words
The method of claim 1 wherein said copolymer is present in said solution in a concentration of from about 8 to about 14% w/v.
Claim 14
Dependent Claim
24 words
The method of claim 1 wherein said copolymer is present in said solution in a concentration of from about 1.5 to about 6% w/v.
Claim 15
Dependent Claim
14 words
The method of claim 11 wherein said concentration is from about 10 to 12%.
Claim 16
Dependent Claim
14 words
The method of claim 12 wherein said concentration is from about 2 to 5%.
Claim 17
Dependent Claim
14 words
The method of claim 12 wherein said concentration is from about 2.5 to 4%.
Claim 18
Dependent Claim
11 words
The method of claim 1 wherein the crosslinking agent is dithiothreitol.
Claim 19
Dependent Claim
12 words
A method according to claim 1 wherein said haloacetyl is a haloacetamido.
Claim 20
Dependent Claim
12 words
A method according to claim 17 wherein said crosslinking agent is dithiothreitol.
Claim 21
Dependent Claim
11 words
A method according to claim 18 wherein said haloacetylamido is chloroacetylamido.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-12-16 | 1995-06-17 | 1995-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-12-16 | 1999-06-17 | 1999-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-12-16 | 2003-06-17 | 2003-12-17 | $7700.00 | Window Closed / Lapsed |