US4704198A
Expired / Lapsed
US
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Porosity gradient electrophoresis gel
Assignee: Revvity Health Sciences Inc
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Filing: 1984-04-27
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Granted: 1987-11-03
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Expires: 2004-04-27
Abstract
Abstract Describes a process for controlling the polymerization and cross-linked density of electrophoretic gel products useful for separation of bioorganic molecules, which process does not use initiators common to processes of the art. Electron beam polymerized gels afford the desired advantages of being ultra thin and having a high electrophoretic resolution with programmable porosity profiles.
Inventors
- 👤 Richard C. Ebersole
- 👤 Robert P. Foss
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 10:10
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; Y; Y10; Y10S; Y10S522/00; Y10S522/91
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
109 words
A porous electrophoretic gel product comprising an aqueous-swelled porous polymer matrix formed of homo- or co-polymers which defines a volume, the polymer matrix being formed from water soluble, ethylenically unsaturated monomers which can undergo radical initiated polymerization, the product characterized by having a constant atomic composition over its volume, an absence of a polymerization catalyst, being stable, having a controlled, electrophoretic resolving capacity that is reproducible from gel to gel for the electrophoretic separation of charged macromolecular substances, having length, width and thickness dimensions, and having a porosity gradient along one of the length and width dimensions and uniform porosity along the thickness dimension which dimension is relatively thin.
Claim 2
Dependent Claim
109 words
A porous electrophoretic gel product comprising an aqueous-swelled porous polymer matrix formed of homo- or co-polymers which defines a volume, the polymer matrix being formed from water soluble, ethylenically unsaturated monomers which can undergo radical initiated polymerization, the product characterized by having a constant atomic composition over its volume, an absence of a polymerization catalyst, being stable, having a controlled, electrophoretic resolving capacity that is reproducible from gel to gel for the electrophoretic separation of charged macromolecular substances, having length, width and thickness dimensions, and having a porosity gradient along one of the length and width dimensions and uniform porosity along the thickness dimension which dimension is relatively thin.
Claim 3
Dependent Claim
37 words
The gel product set forth in claim 1 in which the gel product is a co-polymer consisting essentially of an acrylamide and a monomer selected from the group consisting of N,N'-methylene-bis-acrylamide, N,N'-diallyltartramide, ethylenediacrylate, N,N'-bis-acrylylcystamine, N,N'-(1,2-dihydroxyethylene)bisacrylamide and (polyoxyethyl-trimethylolpropanetriacylate).
Claim 4
Dependent Claim
18 words
The gel product set forth in claim 1 wherein the polymerization is initiated by ionizing radiation induced radicals.
Claim 5
Dependent Claim
29 words
The gel product set forth in claim 1 which also includes one of the group consisting essentially of ampholytes and a mixture of buffers for use in isoelectric focussing.
Claim 6
Dependent Claim
17 words
The gel product set forth in claim 4 wherein the weight/volume percent of polyacrylamide is about 5.
Claim 7
Dependent Claim
18 words
The gel product set forth in claim 1 wherein the porosity gradient is represented by a mathematical function.
Claim 8
Dependent Claim
22 words
The gel product set forth in claim 7 wherein the porosity gradient in one of the length and width dimensions is discontinuous.
Claim 9
Dependent Claim
22 words
The gel product set forth in claim 6 wherein the porosity gradient in one of the length and width dimensions is logarithmic.
Claim 10
Dependent Claim
32 words
The gel product set forth in claim 1 wherein the electrophoretic molecular resolving power is established by a gradient defined by said polymer matrix having varying chain lengths, branching and cross-link densities.
Claim 11
Dependent Claim
24 words
The gel product set forth in claim 6 which has a constant thickness lying in the range from about 50 micrometers to 2 millimeters.
Claim 12
Dependent Claim
30 words
The gel product set forth in claim 1 wherein the thickness of the gel product is constant and lies in the range from about 100 microns to about 300 microns.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-02 | 1991-05-04 | 1991-11-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-02 | 1995-05-04 | 1995-11-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-02 | 1999-05-04 | 1999-11-03 | $7700.00 | Window Closed / Lapsed |