US4810633A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzymatic ethanol test
Assignee: Bayer Corp
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Filing: 1984-06-04
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Granted: 1989-03-07
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Expires: 2004-06-04
Abstract
Abstract The present invention provides a stable, convenient solid state test device for the determination of ethanol in an aqueous test sample, a method for its preparation and a method for its use. The use of carrier matrix incorporated with alcohol oxidase, a peroxidatively active substance and a suitable chromogenic indicator for the determination of ethanol in aqueous samples such as body fluid samples (e.g., serum, urine or saliva) is part of the invention. The invention provides a method of incorporating the enzyme alcohol oxidase and a peroxidatively active substance into a carrier matrix with a chromogenic indicator system capable of providing a detectable response to the presence of at least 100 mg/dL ethanol in less than about 5 minutes. The method involves either (a) the use of a specialized incorporation procedure or (b) the addition of an azide to the test device. Either method overcomes the "false positive" problem seen when solution assay reagents are incorporated at the increased concentrations necessary to provide a test device sensitive to the at least 100 mg/dL ethanol.
Inventors
- 👤 Robert Bauer
- 👤 Thomas A. Magers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 08:56
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
107 words
A dry solid state reagent test device for determining ethanol in body fluids in concentrations as low as 100 mg/dL, capable of providing an analytical colorimetric response in less than 5 minutes, said device comprising: (a) a carrier matrix; and (b) a test composition incorporated into the carrier matrix, said test composition including (i) a chromogenic indicator system capable of providing a colorimetric response, wherein a chromogenic component of the indicator system is substantially in the reduced form; (ii) a peroxidatively active substance; (iii) from about 0.5 to about 5 mM alkali metal azide; and (iv) from about 20 to about 200 IU per milliliter alcohol oxidase.
Claim 2
Dependent Claim
107 words
A dry solid state reagent test device for determining ethanol in body fluids in concentrations as low as 100 mg/dL, capable of providing an analytical colorimetric response in less than 5 minutes, said device comprising: (a) a carrier matrix; and (b) a test composition incorporated into the carrier matrix, said test composition including (i) a chromogenic indicator system capable of providing a colorimetric response, wherein a chromogenic component of the indicator system is substantially in the reduced form; (ii) a peroxidatively active substance; (iii) from about 0.5 to about 5 mM alkali metal azide; and (iv) from about 20 to about 200 IU per milliliter alcohol oxidase.
Claim 3
Dependent Claim
17 words
The test device of claim 1 in which the chromogenic indicator system is a single indicator component.
Claim 4
Dependent Claim
29 words
The test device of claim 2 in which the test composition additionally includds a buffer capable of providing a pH in the range of from about 5 to 9.
Claim 5
Dependent Claim
16 words
The test device of claim 3 in which the test composition additionally includes a shelf-like stabilizer.
Claim 6
Dependent Claim
44 words
A process for determining the presence of ethanol in an aqueous test sample, the process comprising contacting the test sample with the test device of claim 1 and observing in less than 5 minutes any detectable colorimetric response associated with the presence of ethanol.
Claim 7
Independent Claim
131 words
A method for the manufacture of a dry solid state reagent strip for determining ethanol in body fluids in concentrations as low as 100 mg/dL anc capable of providing an anlytical, colorimetric response in less than 5 minutes, said method comprising the steps of: (a) preparing a first mixture of a single component chromogenic indicator and an organic solvent; (b) preparing an aqueous second mixture of a peroxidatively active substance, any remaining components of the chromogenic indicator system, from about 0.5 to about 5 mM alkali metal azide and from about 20 to about 200 IU per milliliter alcohol oxidase; (c) incorporating the carrier matrix with one of the first or second mixtures and drying; and (d) incorporating the carrier with the other of the first or second mixtures and drying.
Claim 8
Independent Claim
131 words
A method for the manufacture of a dry solid state reagent strip for determining ethanol in body fluids in concentrations as low as 100 mg/dL anc capable of providing an anlytical, colorimetric response in less than 5 minutes, said method comprising the steps of: (a) preparing a first mixture of a single component chromogenic indicator and an organic solvent; (b) preparing an aqueous second mixture of a peroxidatively active substance, any remaining components of the chromogenic indicator system, from about 0.5 to about 5 mM alkali metal azide and from about 20 to about 200 IU per milliliter alcohol oxidase; (c) incorporating the carrier matrix with one of the first or second mixtures and drying; and (d) incorporating the carrier with the other of the first or second mixtures and drying.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-03-06 | 1992-09-05 | 1993-03-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-03-06 | 1996-09-05 | 1997-03-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-03-06 | 2000-09-05 | 2001-03-07 | $7700.00 | Window Closed / Lapsed |