US4492754A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Composition and method for the determination of hydrogen peroxide
Assignee: Roche Diagnostics GmbH
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Filing: 1982-06-11
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Granted: 1985-01-08
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Expires: 2002-06-11
Abstract
Abstract Composition for the detection of (a) hydrogen peroxide or hydrogen peroxide forming substrates or (b) peroxidases or peroxidatively reacting substances which composition comprises a substrate capable of coupling and an oxidizable substance known to undergo the Trinder reaction, and (a) a peroxidase or (b) hydrogen peroxide wherein the substrate capable of coupling is a compound of the formula <IMAGE> (I) in which n and m are individually selected from whole numbers of 1 to 4, X and Y, which can be the same or different represent a valence bond or a phenylene radical, R1 and R2 are individually selected from carboxyl or sulfonic acid groups and one of R1 and R2 can also be hydrogen or lower alkyl, and R3 and R4 are individually selected from hydrogen and alkyl radicals of up to 6 carbon atoms; and/or a salt of such compound. The present invention also provides a method wherein the composition is brought together with a sample and the coloration obtained is evaluated visually or with a photometer.
Inventors
- 👤 Ulrich Trager
- 👤 Bernward Sojka
- 👤 Hans Lange
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 14:46
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/20; Y10T436/206664
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
141 words
Composition for the detection of (a) hydrogen peroxide or hydrogen peroxide forming substrates, or (b) peroxidases or peroxidatively reacting substances, which composition comprises a substrate capable of coupling 2-hydrazono-2,3-dihydro-3-methylbenzthiazole-6-sulfonic acid (S-MBTH), S-MBTH, and (a) a peroxidase or (b) hydrogen peroxide and hydrogen peroxide forming substance, wherein the substrate capable of coupling is a compound of the formula ##STR3## in which n and m are individually selected from whole numbers of 1 to 4, X and Y, which can be the same or different, represent a valence bond or a phenylene radical, R 1 and R 2 are individually selected from carboxyl or sulfonic acid groups and one of R 1 and R 2 can also be hydrogen or lower alkyl, R 3 is alkyl or up to 6 carbon atoms; and R 4 is hydrogen; or a salt of such compound.
Claim 2
Dependent Claim
141 words
Composition for the detection of (a) hydrogen peroxide or hydrogen peroxide forming substrates, or (b) peroxidases or peroxidatively reacting substances, which composition comprises a substrate capable of coupling 2-hydrazono-2,3-dihydro-3-methylbenzthiazole-6-sulfonic acid (S-MBTH), S-MBTH, and (a) a peroxidase or (b) hydrogen peroxide and hydrogen peroxide forming substance, wherein the substrate capable of coupling is a compound of the formula ##STR3## in which n and m are individually selected from whole numbers of 1 to 4, X and Y, which can be the same or different, represent a valence bond or a phenylene radical, R 1 and R 2 are individually selected from carboxyl or sulfonic acid groups and one of R 1 and R 2 can also be hydrogen or lower alkyl, R 3 is alkyl or up to 6 carbon atoms; and R 4 is hydrogen; or a salt of such compound.
Claim 3
Dependent Claim
21 words
Composition as claimed in claim 1, wherein in formula (I) X is a valence bond and Y is a phenylene radical.
Claim 4
Dependent Claim
16 words
Composition as claimed in claim 1, wherein in formula (I) X and Y are valence bonds.
Claim 5
Dependent Claim
16 words
Composition as claimed in claim 1, wherein in formula (I) X and Y are phenylene radicals.
Claim 6
Dependent Claim
19 words
Composition as claimed in claim 1, wherein one of R 1 and R 2 in formula (I) is carboxyl.
Claim 7
Dependent Claim
22 words
Composition as claimed in claim 1, wherein one of R 1 and R 2 in formula (I) is a sulfonic acid group.
Claim 8
Dependent Claim
14 words
Composition as claimed in claim 1, wherein in formula (I) R 1 is hydrogen.
Claim 9
Dependent Claim
15 words
Composition as claimed in claim 1, wherein in formula (I) R 1 is lower alkyl.
Claim 10
Dependent Claim
16 words
Composition as claimed in claim 1, wherein the components thereof are impregnated onto an absorbent carrier.
Claim 11
Dependent Claim
20 words
Composition as claimed in claim 1, additionally containing a reagent which releases hydrogen peroxide from a hydrogen peroxide forming substrate.
Claim 12
Dependent Claim
20 words
Composition as claimed in claim 10, wherein the reagent which forms hydrogen peroxide with the substrate is a substrate oxidase.
Claim 13
Dependent Claim
61 words
Method for the detection of (a) hydrogen peroxide or of hydrogen peroxide forming substrates or (b) peroxidases or peroxidatively reacting substrates which method comprises bringing a sample in contact with a composition as claimed in claim 1 and evaluating the coloration obtained as a measure of the content of hydrogen peroxide or hydrogen forming substrate or peroxidase or peroxidatively reacting substrate.
Claim 14
Dependent Claim
12 words
Method as claimed in claim 12, wherein said coloration is evaluated visually.
Claim 15
Dependent Claim
14 words
Method as claimed in claim 12, wherein said coloration is evaluated with a photometer.
Claim 16
Dependent Claim
34 words
Method as claimed in claim 12, wherein the composition is impregnated onto an absorbent carrier and the composite is briefly dipped into a sample until the reagents are eluted, and then determining the coloration.
Claim 17
Dependent Claim
29 words
Method as claimed in claim 12, wherein the composition is impregnated onto an absorbent carrier and the composite is briefly dipped into a sample, the coloration being thereafter determined.
Claim 18
Dependent Claim
22 words
Method as claimed in claim 16, wherein the coloration is determined after dilution of the reaction mixture or passing same through oxygen.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-08 | 1988-07-09 | 1989-01-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-08 | 1992-07-09 | 1993-01-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-08 | 1996-07-09 | 1997-01-08 | $7700.00 | Window Closed / Lapsed |