US5405618A — Biomosaic polymer obtained by emulsion polymeriza…

Claim 1

Independent Claim 300 words

A porous, polymeric membrane, comprising: a polymer formed from at least one monomer consisting essentially of hydrophobic polymerizable addition-polymerizable unsaturated organic compound having at least one double bond between two carbon atoms and having a solubility in water of less than 1 part in 100 parts (weight/weight) of water; said polymer having porous surfaces, with pores of from about 0.01 micrometers to about 10 micrometers, capable of diffusing aqueous solutions without difficulty through the porous, polymeric membrane, and said polymer having at least a minimally useful amount of at least one biochemical compound, irreversibly bound at and becoming a part of said porous surfaces of said polymer during formation of said polymer and biologically active, said surfaces, comprising outer surfaces of said membrane and surfaces of said pores of said membrane, having an elemental content including elements of said polymer and elements of said biochemical compound wherein said biochemical compound is congregated at said surfaces for reaction with compounds in the aqueous solutions, wherein the porous, polymeric membrane is prepared from a polymerization of a water-in-oil or bicontinuous emulsion comprising said hydrophobic polymerizable monomer, said biochemical compound and at least one surface active agent; wherein said polymerization occurs at no less than about ambient temperature; wherein said hydrophobic polymerizable monomer comprises from about 1 to about 97 weight percent of said emulsion; wherein said biochemical compound is in a hydrophilic liquid capable of forming an emulsion or microemulsion with the hydrophobic polymerizable monomer, the amount of said biochemical compound in said hydrophilic liquid is less than saturation amount, and said hydrophilic liquid comprises of from about 1 to about 97 percent of said emulsion; and wherein said surface active agent is in said emulsion in an amount of from about 2 weight percent to about 97 percent of said emulsion.

Claim 2

Dependent Claim 300 words

A porous, polymeric membrane, comprising: a polymer formed from at least one monomer consisting essentially of hydrophobic polymerizable addition-polymerizable unsaturated organic compound having at least one double bond between two carbon atoms and having a solubility in water of less than 1 part in 100 parts (weight/weight) of water; said polymer having porous surfaces, with pores of from about 0.01 micrometers to about 10 micrometers, capable of diffusing aqueous solutions without difficulty through the porous, polymeric membrane, and said polymer having at least a minimally useful amount of at least one biochemical compound, irreversibly bound at and becoming a part of said porous surfaces of said polymer during formation of said polymer and biologically active, said surfaces, comprising outer surfaces of said membrane and surfaces of said pores of said membrane, having an elemental content including elements of said polymer and elements of said biochemical compound wherein said biochemical compound is congregated at said surfaces for reaction with compounds in the aqueous solutions, wherein the porous, polymeric membrane is prepared from a polymerization of a water-in-oil or bicontinuous emulsion comprising said hydrophobic polymerizable monomer, said biochemical compound and at least one surface active agent; wherein said polymerization occurs at no less than about ambient temperature; wherein said hydrophobic polymerizable monomer comprises from about 1 to about 97 weight percent of said emulsion; wherein said biochemical compound is in a hydrophilic liquid capable of forming an emulsion or microemulsion with the hydrophobic polymerizable monomer, the amount of said biochemical compound in said hydrophilic liquid is less than saturation amount, and said hydrophilic liquid comprises of from about 1 to about 97 percent of said emulsion; and wherein said surface active agent is in said emulsion in an amount of from about 2 weight percent to about 97 percent of said emulsion.

Claim 3

Dependent Claim 48 words

The porous, polymeric membrane according to claim 1, wherein said hydrophobic polymerizable monomer comprises a monofunctional acrylate, a multifunctional acrylate, or combinations thereof; wherein said surface active agent is in said emulsion in an amount of from about 10 weight percent to about 60 percent of said emulsion.

Claim 4

Dependent Claim 47 words

The porous, polymeric membrane according to claim 2, wherein said biochemical compound comprises at least one amino acid, carbohydrate, lipid, nucleic acid, protein, antibody, antigenic substance, enzyme, co-factor, inhibitor, lectin, hormone, receptor, coagulation factor, growth enhancer, histone, vitamin, drug, cell surface marker, herbicide, pesticide, or combinations thereof.

Claim 5

Dependent Claim 62 words

The porous, polymeric membrane according to claim 3, wherein said hydrophobic polymerizable monomer comprises isooctyl acrylate, isobornyl acrylate, 2-ethylhexyl acrylate, ethylenediglycol acrylate, ethyltriglycol methacrylate, 1,6-hexanediol diacrylate, tetraethyleneglycol diacrylate, tripropyleneglycol diacrylate, propoxylated glycol diacrylate, pentaerythritol triacrylate, trimethylolpropane triacrylate, pentaerythritol tetraacrylate, di-trimethylolpropane tetraacrylate or combinations thereof in said emulsion in an amount of from about 25 to about 80 weight percent of said emulsion.

Claim 6

Dependent Claim 44 words

The porous, polymeric membrane according to claim 3, wherein from about 5 percent to about 75 percent of said biochemical compound in said emulsion during said polymerization of said hydrophobic polymerizable monomer is irreversibly bound to said surfaces of said polymer after said polymerization.

Claim 7

Dependent Claim 43 words

The porous, polymeric membrane according to claim 3, wherein as little of said biochemical compound as is minimally useful to as much of said biochemical compound as about 70 μg/cm 2 of said membrane is bound at said surfaces of said porous membrane.

Claim 8

Dependent Claim 42 words

The porous, polymeric membrane according to claim 3, wherein as little of said biochemical compound as is minimally useful to as much of said biochemical compound as about 20 mg/g of said polymer is bound at said surfaces of said porous membrane.

Claim 9

Dependent Claim 33 words

The porous, polymeric membrane according to claim 3, wherein said biochemical compound comprises between as little of said surfaces as is minimally useful to as much of said surfaces as about 84 percent.

Claim 10

Independent Claim 168 words

A method for the preparation of a polymeric membrane comprising: mixing a water-in-oil or bicontinuous emulsion comprising at least a minimally useful amount of at least one biochemical compound, at least one hydrophilic liquid, at least one hydrophobic polymerizable monomer consisting essentially of at least one hydrophobic addition-polymerizable unsaturated organic compound having at least one double bond between two carbon atoms having a solubility in water of less than one part in 100 parts (weight/weight) of water, and at least one surface active agent; and polymerizing said emulsion at no lower than about ambient temperature to polymerize said hydrophobic polymerizable monomer to form a porous, polymeric membrane having said biochemical compound, irreversibly bound at and becoming part of the surfaces of said membrane during formation of said membrane and biologically active, said surfaces having an elemental content including elements of said polymer and elements of said biochemical compound wherein said biochemical compound is congregated at said surfaces in biologically active condition for biochemical, immunochemical, physiological or pharmaceutical reaction.

Claim 11

Independent Claim 168 words

A method for the preparation of a polymeric membrane comprising: mixing a water-in-oil or bicontinuous emulsion comprising at least a minimally useful amount of at least one biochemical compound, at least one hydrophilic liquid, at least one hydrophobic polymerizable monomer consisting essentially of at least one hydrophobic addition-polymerizable unsaturated organic compound having at least one double bond between two carbon atoms having a solubility in water of less than one part in 100 parts (weight/weight) of water, and at least one surface active agent; and polymerizing said emulsion at no lower than about ambient temperature to polymerize said hydrophobic polymerizable monomer to form a porous, polymeric membrane having said biochemical compound, irreversibly bound at and becoming part of the surfaces of said membrane during formation of said membrane and biologically active, said surfaces having an elemental content including elements of said polymer and elements of said biochemical compound wherein said biochemical compound is congregated at said surfaces in biologically active condition for biochemical, immunochemical, physiological or pharmaceutical reaction.

Claim 12

Dependent Claim 45 words

The method according to claim 9, wherein said biochemical compound comprises at Least one amino acid, carbohydrate, lipid, nucleic acid, protein, antibody, antigenic substance, enzyme, co-factor, inhibitor, lectin, hormone, receptor, coagulation factor, growth enhancer, histone, vitamin, drug, cell surface marker, herbicide, pesticide, or combinations thereof.

Claim 13

Dependent Claim 23 words

The method according to claim 9, where said hydrophobic polymerizable monomer comprises from about 1 to about 97 weight percent of said emulsion.

Claim 14

Dependent Claim 38 words

The method according to claim 11, wherein said hydrophobic polymerizable monomer comprises a monofunctional acrylate, multifunctional acrylate, or combinations thereof in said emulsion in an amount of from about 25 to about 80 weight percent of said emulsion.

Claim 15

Dependent Claim 42 words

The method according to claim 11, wherein said hydrophobic polymerizable monomer comprises isooctyl acrylate, isobornyl acrylate, 2-ethylhexyl acrylate, ethylenediglycol acrylate, ethyltriglycol methacrylate, 1,6-hexanediol diacrylate, tetraethyleneglycol diacrylate, tripropyleneglycol diacrylate, propoxylated glycol diacrylate, pentaerythritol triacrylate, trimethylolpropane triacrylate, pentaerythritol tetraacrylate, di-trimethylolpropane tetraacrylate or combinations thereof.

Claim 16

Dependent Claim 90 words

The method according to claim 9, further comprising the step of placing said biochemical compound in said hydrophilic liquid prior to said mixing step, wherein the amount of said biochemical compound in said hydrophilic liquid is less than saturation amount, wherein said hydrophilic liquid comprises of from about 1 to about 97 weight percent of said emulsion, and wherein from about 1 percent to about 90 percent of said biochemical compound in said emulsion during said polymerization of said polymerizable monomer is irreversibly bound at said surfaces after said polymerization.

Claim 17

Dependent Claim 37 words

The method according to claim 14, wherein from about 5 percent to about 75 percent of said biochemical compound in said emulsion during said polymerization of said polymerizable monomer is bound at said surfaces after said polymerization.

Claim 18

Dependent Claim 184 words

The method according to claim 9, wherein said surface active agent comprises at least one ionic surface active agent, nonionic surface active agent, zwitterionic surface active agent, or combinations thereof in said emulsion in an amount of from about 2 weight percent to about 97 weight percent of said emulsion; wherein said ionic surface active agent comprises a salt of a carboxylic acid having a carbon chain length of from about 8 to about 22 atoms, a sulfonic acid, a sulfuric acid ester, a phosphoric acid ester, a polyphosphoric acid ester, a quarternary ammonium salt, an oxide or salt of an amine compound having a carbon chain length of from about 8 to about 22 atoms, or a combination thereof; wherein said nonionic surface active agent comprises a polyoxyethylenated-alkyl phenol, a polyoxyethylenated-straight chain alcohol, a polyoxyethylenated-polypropylene glycol, a carboxylic acid ester having a carbon chain length of from about 8 to 22 atoms, or a combination thereof; and wherein said zwitterionic surface active agent comprises a beta-N-alkylaminopropionic acid, a N-alkyl-beta-iminodipropionic acid, an imidazoline carboxylate, a N-alkylbetaine, a sulfobetaine, a sultaine or a combination thereof.

Claim 19

Dependent Claim 31 words

The method according to claim 9, further comprising, between the steps of said mixing and said polymerizing, the step of dispensing said emulsion into a desired form prior to said polymerizing.

Claim 20

Dependent Claim 17 words

The method according to claim 9, wherein said polymerizing comprises irradiating said emulsion with high energy radiation.

Claim 21

Dependent Claim 20 words

A method for the preparation of a biomosaic polymer according to claim 18, wherein said high-energy radiation is ultra-violet radiation.

Claim 22

Dependent Claim 16 words

The porous, polymeric membrane, according to claim 2, wherein the porous membrane comprises means for immunoassay.

Claim 23

Dependent Claim 16 words

The porous, polymeric membrane, according to claim 2, wherein the porous membrane comprises an enzyme reactor.

Claim 24

Dependent Claim 15 words

The porous, polymeric membrane, according to claim 2, wherein the porous membrane comprises a bioseparator.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1998-04-10	1998-10-10	1999-04-11	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	2002-04-10	2002-10-10	2003-04-11	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	2006-04-10	2006-10-10	2007-04-11	$7700.00	Window Closed / Lapsed

Biomosaic polymer obtained by emulsion polymerization of hydrophobic monomers in the presence of bioactive materials

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