EP0226470A2
Expired / Lapsed
EP
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Materials and methods for microchemical testing
Assignee: Unilever PLC
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Filing: 1986-12-12
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Granted: —
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Expires: 2006-12-12
Abstract
Abstract Apparatus for carrying out a microchemical test, comprising a solid substrate having a surface which carries a polymer hydrogel formed in situ thereon and covalently bonded thereto, for example with the gel in contact with a metal conferring microchemical analytical specificity on the apparatus, e.g. an immunological reactant or an electrode. For example, such a gel in a layer carried within a capillary-fill cell can be used for optical immunoassay.
Inventors
- 👤 John Anthony Bosley
- 👤 Paul James Davis
- 👤 Ian Alexander Shanks
- 👤 Alan Martin Smith
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 09:03
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; G01N33/544; G01N33/545; G01N33/549
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
27 words
Apparatus for carrying out a microchemical test, comprising a solid substrate having a surface which carries a polymer hydrogel formed in situ thereon and covalently bonded thereto.
Claim 2
Dependent Claim
27 words
Apparatus for carrying out a microchemical test, comprising a solid substrate having a surface which carries a polymer hydrogel formed in situ thereon and covalently bonded thereto.
Claim 3
Dependent Claim
21 words
Apparatus according to claim 1, wherein said gel is in contact with a material conferring microchemical analytical specificity on said apparatus.
Claim 4
Dependent Claim
21 words
Apparatus according to claim 2, wherein said gel carries an immunological reagent which is a ligand or a binding agent therefor.
Claim 5
Dependent Claim
23 words
Apparatus according to claim 2, wherein said gel is crosslinked and said ligand or binding agent is a macromolecule occluded within said gel.
Claim 6
Dependent Claim
17 words
Apparatus according to claim 3, wherein said ligand or binding agent is covalently bonded to said gel.
Claim 7
Dependent Claim
30 words
Apparatus according to claim 1, comprising a siliceous substrate surface-coated with an organic trialkoxy silane derivative which carries an organic functional group conjugated with a mer of the polymer hydrogel.
Claim 8
Dependent Claim
64 words
Apparatus according to claim 6, wherein the polymer hydrogel is a cross-linked acrylic polymer hydrogel covalently linked to said surface by polymerisation in situ via residues of said organic trialkoxysilane carrying residues of amino-alkyl or epoxyalkyl or acryloyl groups and optionally carrying organic functional groups derived from comonomers which are copolymerisable with acrylic monomers and which comprise N- hydroxysuccinimidyl, hydroxyethyl, carboxyl or aldehyde groups.
Claim 9
Dependent Claim
19 words
Apparatus according to claim 1, wherein said solid substrate is a plate carrying a layer of said gel thereon.
Claim 10
Dependent Claim
24 words
Apparatus according to claim 8, wherein said gel is carried on an internal surface of a capillary-fill cell formed by two closely-spaced parallel plates.
Claim 11
Dependent Claim
24 words
Apparatus according to claim 1, wherein said surface of said solid substrate carries material in contact with said gel and forming an electrochemical electrode.
Claim 12
Dependent Claim
33 words
Apparatus according to claim 1, wherein said gel carries at least one enzyme and at least one ligand or receptor therefor, optionally with a chromogenic or fluorogenic enzyme substrate occluded within said gel.
Claim 13
Dependent Claim
68 words
A process for producing a gel carried on a surface of a solid substrate, which comprises: (a) activating the surface of the substrate to provide covalently-attached functional groups which are capable of reacting with a polymerising monomer, and (b) poymerising a polymerisable monomer solution in a layer in contact with said activated surface, thereby to form in situ a layer of polymer gel covalently linked to said surface.
Claim 14
Dependent Claim
68 words
A process for producing a gel carried on a surface of a solid substrate, which comprises: (a) activating the surface of the substrate to provide covalently-attached functional groups which are capable of reacting with a polymerising monomer, and (b) poymerising a polymerisable monomer solution in a layer in contact with said activated surface, thereby to form in situ a layer of polymer gel covalently linked to said surface.
Claim 15
Dependent Claim
18 words
A process according to claim 12, wherein the monomer solution is an aqueous preparation of polymerisable acrylic monomer.
Claim 16
Dependent Claim
21 words
A process according to claim 13, wherein a ligand or specific ligand binding agent is present in the aqueous monomer preparation.
References Cited (Backward Citations)
No citation records indexed.
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