US5358844A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preservation of blood platelets
Assignee: The Brigham and Women's Hospital Inc
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Filing: 1993-02-18
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Granted: 1994-10-25
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Expires: 2013-02-18
Abstract
Abstract Methods for preserving platelets at temperatures of less than about 15 DEG C. with retention of hemostatic activity are provided. The method uses a first agent for inhibiting actin filament severing and a second agent for inhibiting actin polymerization. Contacting the platelets with the first and second agents prior to exposure to cold temperature prevents cold-induced platelet activation.
Inventors
- 👤 Thomas P. Stossel
- 👤 John H. Hartwig
- 👤 Paul A. Janmey
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 03:59
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A01N1/128; A01N1/14; A01N1/146; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/106664; Y10T436/108331
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
63 words
A method for the preservation of platelets with preserved hemostatic activity, the method comprising the steps of: (a) contacting a preparation of platelets with a first agent for inhibiting actin filament severing and with a second agent for inhibiting actin polymerization to form a treated platelet preparation; and (b) storing said treated platelet preparation at a temperature of less than about 15° C.
Claim 2
Independent Claim
63 words
A method for the preservation of platelets with preserved hemostatic activity, the method comprising the steps of: (a) contacting a preparation of platelets with a first agent for inhibiting actin filament severing and with a second agent for inhibiting actin polymerization to form a treated platelet preparation; and (b) storing said treated platelet preparation at a temperature of less than about 15° C.
Claim 3
Dependent Claim
23 words
A method as claimed in claim 1, wherein said first agent and said second agent are sequentially contacted with said preparation of platelets.
Claim 4
Dependent Claim
29 words
A method as claimed in claim 2, wherein said first agent is contacted with said preparation of platelets prior to contacting said second agent with said preparation of platelets.
Claim 5
Dependent Claim
16 words
A method as claimed in claim 1, wherein said first agent is an intracellular calcium chelator.
Claim 6
Dependent Claim
36 words
A method as claimed in claim 4, wherein said intracellular calcium chelator is a lipophilic derivative of a calcium chelator selected from the group consisting of quin-1, quin-2, stil-1, stil-2, indo-1, fura-1, fura-2, fura-3, and BAPTA.
Claim 7
Dependent Claim
18 words
A method as claimed in claim 5, wherein said intracellular calcium chelator is a lipophilic derivative of quin-2.
Claim 8
Dependent Claim
18 words
A method as claimed in claim 6, wherein said intracellular calcium chelator is an acetoxymethyl ester of quin-2.
Claim 9
Dependent Claim
25 words
A method as claimed in claim 1, wherein said second agent is selected from the group consisting of cytochalasin B, dihydro-cytochalasin B and cytochalasin D.
Claim 10
Dependent Claim
14 words
A method as claimed in claim 8, wherein said second agent is cytochalasin B.
Claim 11
Independent Claim
91 words
A method for making a pharmaceutical composition for administration to a mammal, the method comprising the steps of: (a) contacting a preparation of platelets contained in a pharmaceutically-acceptable carrier with a first agent for inhibiting actin filament severing and with a second agent for inhibiting actin polymerization to form a treated platelet preparation; (b) storing said treated platelet preparation at a temperature of less than about 15° C. to form a platelet preparation; (c) warming said platelet preparation; and (d) neutralizing said first agent and second agent in said platelet preparation.
Claim 12
Independent Claim
91 words
A method for making a pharmaceutical composition for administration to a mammal, the method comprising the steps of: (a) contacting a preparation of platelets contained in a pharmaceutically-acceptable carrier with a first agent for inhibiting actin filament severing and with a second agent for inhibiting actin polymerization to form a treated platelet preparation; (b) storing said treated platelet preparation at a temperature of less than about 15° C. to form a platelet preparation; (c) warming said platelet preparation; and (d) neutralizing said first agent and second agent in said platelet preparation.
Claim 13
Dependent Claim
24 words
A method as claimed in claim 10, wherein the step of warming said preparation of platelets is by warming the platelets to 37° C.
Claim 14
Dependent Claim
29 words
A method as claimed in claim 10, wherein said first agent is contacted with said preparation of platelets prior to contacting said second agent with said preparation of platelets.
Claim 15
Dependent Claim
16 words
A method as claimed in claim 10, wherein said first agent is an intracellular calcium chelator.
Claim 16
Dependent Claim
36 words
A method as claimed in claim 13, wherein said intracellular calcium chelator is a lipophilic derivative of a calcium chelator selected from the group consisting of quin-1, quin-2, stil-1, stil-2, indo-1, fura-1, fura-2, fura-3, and BAPTA.
Claim 17
Dependent Claim
18 words
A method as claimed in claim 14, wherein said intracellular calcium chelator is a lipophilic derivative of quin-2.
Claim 18
Dependent Claim
25 words
A method as claimed in claim 10, wherein said second agent is selected from the group consisting of cytochalasin B, dihydro-cytochalasin B and cytochalasin D.
Claim 19
Dependent Claim
14 words
A method as claimed in claim 16, wherein said second agent is cytochalasin B.
Claim 20
Dependent Claim
20 words
A method as claimed in claim 10, wherein neutralizing said first and second agents is by washing said platelet preparation.
Claim 21
Dependent Claim
27 words
A method as claimed in claim 10, wherein neutralizing said first and second agents is by diluting said platelet preparation with a suspension of red blood cells.
Claim 22
Dependent Claim
21 words
A method as claimed in claim 13, wherein neutralizing said first agent is by adding unchelated calcium to said platelet preparation.
Claim 23
Independent Claim
97 words
A method for mediating hemostasis in a mammal, comprising the steps of: (a) contacting a preparation of platelets contained in a pharmaceutically-acceptable carrier with a first agent for inhibiting actin filament severing and with a second agent for inhibiting actin polymerization to form a treated platelet preparation; (b) storing said treated platelet preparation at a temperature of less than about 15° C. to form a platelet preparation; (c) warming the platelet preparation; (d) neutralizing said first and second agents in said platelet preparation to form a pharmaceutical composition; and (e) administering said pharmaceutical composition to the mammal.
Claim 24
Independent Claim
97 words
A method for mediating hemostasis in a mammal, comprising the steps of: (a) contacting a preparation of platelets contained in a pharmaceutically-acceptable carrier with a first agent for inhibiting actin filament severing and with a second agent for inhibiting actin polymerization to form a treated platelet preparation; (b) storing said treated platelet preparation at a temperature of less than about 15° C. to form a platelet preparation; (c) warming the platelet preparation; (d) neutralizing said first and second agents in said platelet preparation to form a pharmaceutical composition; and (e) administering said pharmaceutical composition to the mammal.
Claim 25
Dependent Claim
22 words
A method as claimed in claim 21, wherein the step of warming said platelets is by warming the platelets to 37° C.
Claim 26
Dependent Claim
29 words
A method as claimed in claim 22, wherein said first agent is contacted with said preparation of platelets prior to contacting said second agent with said preparation of platelets.
Claim 27
Dependent Claim
16 words
A method as claimed in claim 21, wherein said first agent is an intracellular calcium chelator.
Claim 28
Dependent Claim
36 words
A method as claimed in claim 24, wherein said intracellular calcium chelator is a lipophilic derivative of a calcium chelator selected from the group consisting of quin-1, quin-2, stil-1, stil-2, indo-1, fura-1, fura-2, fura-3, and BAPTA.
Claim 29
Dependent Claim
18 words
A method as claimed in claim 25, wherein said intracellular calcium chelator is a lipophilic derivative of quin-2.
Claim 30
Dependent Claim
25 words
A method as claimed in claim 21, wherein said second agent is selected from the group consisting of cytochalasin B, dihydro-cytochalasin B and cytochalasin D.
Claim 31
Dependent Claim
14 words
A method as claimed in claim 27, wherein said second agent is cytochalasin B.
Claim 32
Dependent Claim
20 words
A method as claimed in claim 21, wherein neutralizing said first and second agents is by washing said platelet preparation.
Claim 33
Dependent Claim
27 words
A method as claimed in claim 29, wherein neutralizing said first and second agents is by diluting said platelet preparation with a suspension of red blood cells.
Claim 34
Dependent Claim
38 words
A method as claimed in claim 21, wherein neutralizing at least one of said first agent and said second agent is by removing at least one of said first agent and said second agent from said platelet preparation.
Claim 35
Dependent Claim
21 words
A method as claimed in claim 24, wherein neutralizing said first agent is by adding unchelated calcium to said platelet preparation.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-10-24 | 1998-04-25 | 1998-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-10-24 | 2002-04-25 | 2002-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-10-24 | 2006-04-25 | 2006-10-25 | $7700.00 | Window Closed / Lapsed |