US5185160A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Platelet membrane microvesicles
Assignee: PRP Inc A DE CORP
•
Filing: 1989-04-14
•
Granted: 1993-02-09
•
Expires: 2009-04-14
Abstract
Abstract A heat-treated, viral-inactivated platelet membrane microparticle fraction is provided. The microparticles may be prepared from outdated platelets. The microparticle fraction is substantially free of platelet ghosts and may be used as a pharmaceutical preparation in transfusions.
Inventors
- 👤 Francis C. Chao
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 05:24
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/19; A61P; A61P7/00; A61P7/02; A61P7/04
Claims Analysis
Total Claims: 52
Claim 1
Independent Claim
29 words
A method for preparing a platelet membrane fraction for transfusion comprising heating platelets or a membrane fraction thereof to at least 60° C. and isolating the heat-treated membrane fraction.
Claim 2
Independent Claim
29 words
A method for preparing a platelet membrane fraction for transfusion comprising heating platelets or a membrane fraction thereof to at least 60° C. and isolating the heat-treated membrane fraction.
Claim 3
Dependent Claim
30 words
A method as claimed in claim 1 further comprising sonicating said platelets or membrane fraction thereof after heating to form a platelet membrane microparticle fraction substantially free of ghost platelets.
Claim 4
Dependent Claim
48 words
A method as claimed in claim 1 further comprising, first suspending said platelets or membrane fraction thereof in a solution to form a suspension, heating said suspension to at least 60° C., homogenizing said suspension, and separating any precipitate formed as a result of heating from said suspension.
Claim 5
Dependent Claim
14 words
A method as claimed in claim 3 wherein said suspension is homogenized by sonication.
Claim 6
Dependent Claim
40 words
A method as claimed in claim 1 further comprising, first forming a ghost platelet fraction and a lysate from platelets, separating said ghost platelet fraction from said lysate, and then heating said ghost platelet fraction to at least 60° C.
Claim 7
Dependent Claim
25 words
A method as claim in claim 5 further comprising, further homogenizing said ghost platelet fraction to form a microparticle fraction substantially free of ghost platelets.
Claim 8
Dependent Claim
56 words
A method as claimed in claim 6 further comprising, suspending said ghost platelet fraction in a solution to form a suspension prior to heating, heating said suspension to at least 60° C., sonicating said suspension to form a microparticle fraction substantially free of ghost platelets, and separating any precipitate formed during heating from said microparticle fraction.
Claim 9
Dependent Claim
22 words
A method as claimed in claim 5 wherein said platelets are freeze thawed to form said ghost platelet fraction and said lysate.
Claim 10
Dependent Claim
43 words
A method as claimed in claim 1 further comprising, sonicating said platelets or platelet membrane fraction thereof after heating to form a microparticle fraction substantially free of ghosts, and separating any precipitate formed as a result of said heating from said microparticle fraction.
Claim 11
Dependent Claim
22 words
A method as claimed in claim 1 further comprising, removing substantially all of any serotonin from said platelets or membrane fraction thereof.
Claim 12
Dependent Claim
31 words
A method as claimed in claim 1 further comprising disrupting the membrane of whole platelets to form a lysate and a membrane fraction and separating said lysate from said membrane fraction.
Claim 13
Independent Claim
26 words
A method as claimed in claims 1, 2, 3, 9 or 11 further comprising heating outdated platelets or a fraction thereof to at least 60° C.
Claim 14
Independent Claim
29 words
A method for preparing a therapeutic preparation of platelet microparticle fraction comprising disrupting the membranes of outdated platelets to form a platelet microparticle fraction substantially free of ghost platelets.
Claim 15
Independent Claim
29 words
A method for preparing a therapeutic preparation of platelet microparticle fraction comprising disrupting the membranes of outdated platelets to form a platelet microparticle fraction substantially free of ghost platelets.
Claim 16
Dependent Claim
21 words
A method as claimed in claim 13 further characterized by separating substantially all of the cytoplasmic serotonin from the microparticle fraction.
Claim 17
Dependent Claim
42 words
A method as claimed in claim 13 further comprising, disrupting the membranes of the outdated platelets to form a ghost fraction and a lysate, separating said ghost fraction and said lysate, and then sonicating said ghost fraction to form the microparticle fraction.
Claim 18
Dependent Claim
18 words
A method as claimed in claim 15 further comprising, forming the ghost fraction by freeze thawing outdated platelets.
Claim 19
Dependent Claim
24 words
A method as claimed in claim 15 further comprising, heating said ghost fraction to at least 60° C. prior to sonicating said ghost fraction.
Claim 20
Dependent Claim
23 words
A method as claimed in claim 17 wherein the outdated platelets comprise a mixture of outdated platelets obtained from more than one person.
Claim 21
Independent Claim
44 words
A method for preparing platelets for use in transfusion comprising, homogenizing said platelets to form a platelet microparticle fraction substantially free of ghost platelets, separating the microparticle fraction from substantially all of any serotinin released from the whole platelets, and isolating the microparticle fraction.
Claim 22
Independent Claim
44 words
A method for preparing platelets for use in transfusion comprising, homogenizing said platelets to form a platelet microparticle fraction substantially free of ghost platelets, separating the microparticle fraction from substantially all of any serotinin released from the whole platelets, and isolating the microparticle fraction.
Claim 23
Independent Claim
62 words
A method for preparing platelets for use in transfusion comprising, freeze thawing platelets to yield a platelet ghost fraction and a lysate, separating the ghost platelet fraction from the lysate, suspending the ghost platelet fraction in a solution to form a suspension, sonicating the ghost platelet fraction to form a microparticle fraction substantially free of ghost platelets, and isolating the microparticle fraction.
Claim 24
Independent Claim
62 words
A method for preparing platelets for use in transfusion comprising, freeze thawing platelets to yield a platelet ghost fraction and a lysate, separating the ghost platelet fraction from the lysate, suspending the ghost platelet fraction in a solution to form a suspension, sonicating the ghost platelet fraction to form a microparticle fraction substantially free of ghost platelets, and isolating the microparticle fraction.
Claim 25
Independent Claim
24 words
A method for inhibiting bleeding in a mammal comprising administering to a mammal a pharmaceutically-effective amount of a platelet fraction prepared from outdated platelets.
Claim 26
Independent Claim
24 words
A method for inhibiting bleeding in a mammal comprising administering to a mammal a pharmaceutically-effective amount of a platelet fraction prepared from outdated platelets.
Claim 27
Dependent Claim
13 words
A method as claimed in claim 21 wherein said fraction is administered topically.
Claim 28
Dependent Claim
26 words
A method as claimed in claim 21 further comprising heating said outdated platelets or fraction thereof to at least 60° C. prior to administering said fraction.
Claim 29
Dependent Claim
19 words
A method as claimed in claim 21 wherein a microparticle platelet fraction substantially free of ghost platelets is administered.
Claim 30
Independent Claim
41 words
A method for inhibiting bleeding in a mammal comprising, administering to a mammal a pharmaceutically-effective amount of a transfusion material prepared by heating platelets or a membrane fraction thereof to at least 60° C. and then isolating the heat-treated membrane fraction.
Claim 31
Independent Claim
41 words
A method for inhibiting bleeding in a mammal comprising, administering to a mammal a pharmaceutically-effective amount of a transfusion material prepared by heating platelets or a membrane fraction thereof to at least 60° C. and then isolating the heat-treated membrane fraction.
Claim 32
Independent Claim
62 words
A method for inhibiting bleeding in a mammal comprising, administering a pharmaceutically-effective amount of a microparticle fraction to a mammal, the microparticle fraction prepared by homogenizing platelets or a fraction thereof to form a platelet microparticle fraction substantially free of ghost platelets, separating the microparticle fraction from substantially all of any serotinin released during said homogenization step, and isolating the microparticle fraction.
Claim 33
Independent Claim
62 words
A method for inhibiting bleeding in a mammal comprising, administering a pharmaceutically-effective amount of a microparticle fraction to a mammal, the microparticle fraction prepared by homogenizing platelets or a fraction thereof to form a platelet microparticle fraction substantially free of ghost platelets, separating the microparticle fraction from substantially all of any serotinin released during said homogenization step, and isolating the microparticle fraction.
Claim 34
Dependent Claim
14 words
An isolated platelet membrane microparticle fraction substantially free of platelets, platelet ghosts and virus.
Claim 35
Dependent Claim
14 words
An isolated platelet membrane microparticle fraction substantially free of platelets, platelet ghosts and virus.
Claim 36
Dependent Claim
16 words
A platelet membrane microparticle fraction as claimed in claim 27 being substantially free of cytoplasmic serotonin.
Claim 37
Dependent Claim
27 words
A platelet membrane microparticle fraction as claimed in claim 27 having about 3% carbohydrate, 30% phospholipid, 58% protein, and 9% cholesterol as a percentage of total weight.
Claim 38
Dependent Claim
21 words
A platelet membrane microparticle fraction as claimed in claim 27 wherein 80% of the microvesicles are under 600 nanometers in diameter.
Claim 39
Dependent Claim
25 words
A platelet membrane microparticle fraction as claimed in claim 27 wherein the microvesicles have an average diameter of between about 300 nanometers and 400 nanometers.
Claim 40
Dependent Claim
30 words
A platelet membrane microparticle fraction as claimed in claim 27, further characterized in being substantially free of Factors V, VIII, IX and X, of Purine nucleoside phosphorylase, and of GPIIb/IIIa.
Claim 41
Dependent Claim
14 words
A pharmaceutical preparation containing a pharmaceutically-effective amount of the microparticle fraction of claim 27.
Claim 42
Dependent Claim
14 words
A pharmaceutical preparation containing a pharmaceutically-effective amount of the microparticle fraction of claim 28.
Claim 43
Dependent Claim
14 words
A pharmaceutical preparation containing a pharmaceutically-effective amount of the microparticle fraction of claim 29.
Claim 44
Dependent Claim
14 words
A pharmaceutical preparation containing a pharmaceutically-effective amount of the microparticle fraction of claim 30.
Claim 45
Dependent Claim
14 words
A pharmaceutical preparation containing a pharmaceutically-effective amount of the microparticle fraction of claim 31.
Claim 46
Dependent Claim
25 words
A method for preparing a platelet membrane fraction as claimed in claim 1 wherein a heat-treated membrane fraction having procoagulant and hemostatic properties is isolated.
Claim 47
Dependent Claim
30 words
A method for preparing a platelet membrane fraction as claimed in claim 1 wherein a heat-treated membrane fraction capable of achieving hemostasis when introduced into a bleeding mammal is isolated.
Claim 48
Dependent Claim
27 words
A method for preparing a platelet membrane fraction as claimed in claim 1 wherein a heat-treated membrane fraction having a protein:phospholipid content of about 2:1 is isolated.
Claim 49
Dependent Claim
28 words
A method for preparing a platelet membrane fraction as claimed in claim 1 wherein a heat-treated membrane fraction having a protein:phospholipid content of less than 2:1 is isolated.
Claim 50
Dependent Claim
34 words
A method for preparing a platelet membrane fraction as claimed in claim 1 wherein a heat-treated membrane fraction having GPIb activity and substantially free of GPIIb-IIIa and substantially free of plasma proteins is isolated.
Claim 51
Dependent Claim
20 words
A platelet membrane microparticle fraction as claimed in claim 27 wherein the fraction has a protein:phospholipid ratio of about 2:1.
Claim 52
Dependent Claim
21 words
A platelet membrane microparticle fraction as claimed in claim 17 wherein the fraction has a protein:phospholipid ratio of less than 2:1.
References Cited (Backward Citations)
US4456590A
Cited
US4456590B1
Cited
US4456590B2
Cited
US4994438A
Cited
US4540573A
Cited
US4760131A
Cited
US4764463A
Cited
US4753797A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-02-09 | 1996-08-10 | 1997-02-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-02-09 | 2000-08-10 | 2001-02-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-02-09 | 2004-08-10 | 2005-02-09 | $7700.00 | Window Closed / Lapsed |