US3890435A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antibiotic substance
Assignee: Imperial Chemical Industries Ltd
•
Filing: 1971-04-07
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Granted: 1975-06-17
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Expires: 1991-04-07
Abstract
Abstract The new compound known as cytochalasin E and a process for its manufacture by cultivation of the organism Rosellinia Necatrix. The compound produces effects on tissue culture cells and is useful in the study of cytological processes.
Inventors
- 👤 Douglas Broadbent
- 👤 Brian Frederick Burrows
- 👤 Stephen Barnaby Carter
- 👤 Harold George Hemming
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 06:55
CPC Classifications
C; C07; C07D; C07D491/00; C07D491/12; C07D491/20; C12; C12N; C12N1/00; C12N1/14; C12N1/145; C12P; C12P1/00; C12P1/02; C12P17/00; C12P17/18; C12P17/188; C12R; C12R2001/00; C12R2001/645; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
109 words
THE COMPOUND CYTOCHALASIN E, SAID COMPOUND HAVING THE FOLLOWING CHARACTERISTICS: METLING POINT: VARIABLE BETWEEN 200* AND 200*C., BUT COMMONLY 206*-209*C. INFRA-RED SPECTRUM; BANDS HAVING V MAX (IN LIQUID PARAFFIN) AT 3520,3450,3200,3120 (ALL WEAK),1760,1730,1705 (ALL STRONG), 1670, 1640, (BOTH WEAK) CM.-1 ULTRAVIOLET SPECTRUM: NO SELECTIVE ABSORPTION BETWEEN 225 MU AND 350 MU PROTON MAGNETIC RESONANCE SPECTRUM: PEAKS IN THE REGION OF *=2.8 (AROMATIC PROTONS) WHEN MEASURED IN CDCL3 OR C5D5N; PEAKS (DOUBLETS) CENTRED (WHEN MEASURED IN C5D5N) AT *=3.16 AND *4.10 RESPECTIVELY, BOTH HAVING A COUPLING CONSTANT OF 12.0 CYCLES PER SECOND. MASS SPECTROMETRY: HEAVIEST ION OBSERVED:M/E=495, CORRESPONDING TO C28H33N07 OTHER IONS: M/E=337, CORRESPONDING TO C22H27NO2 M/E=246, CORRESPONDING TO C15H20NO2
Claim 2
Dependent Claim
109 words
THE COMPOUND CYTOCHALASIN E, SAID COMPOUND HAVING THE FOLLOWING CHARACTERISTICS: METLING POINT: VARIABLE BETWEEN 200* AND 200*C., BUT COMMONLY 206*-209*C. INFRA-RED SPECTRUM; BANDS HAVING V MAX (IN LIQUID PARAFFIN) AT 3520,3450,3200,3120 (ALL WEAK),1760,1730,1705 (ALL STRONG), 1670, 1640, (BOTH WEAK) CM.-1 ULTRAVIOLET SPECTRUM: NO SELECTIVE ABSORPTION BETWEEN 225 MU AND 350 MU PROTON MAGNETIC RESONANCE SPECTRUM: PEAKS IN THE REGION OF *=2.8 (AROMATIC PROTONS) WHEN MEASURED IN CDCL3 OR C5D5N; PEAKS (DOUBLETS) CENTRED (WHEN MEASURED IN C5D5N) AT *=3.16 AND *4.10 RESPECTIVELY, BOTH HAVING A COUPLING CONSTANT OF 12.0 CYCLES PER SECOND. MASS SPECTROMETRY: HEAVIEST ION OBSERVED:M/E=495, CORRESPONDING TO C28H33N07 OTHER IONS: M/E=337, CORRESPONDING TO C22H27NO2 M/E=246, CORRESPONDING TO C15H20NO2
Claim 3
Dependent Claim
65 words
A process for the manufacture of cytochalasin E as define in claim 1 which comprises cultivation of the strain of the organism Rosellinia necatrix CMI 108,006 in an aqueous nutrient medium a temperature of 15*-35*C. and for a period of up to 21 to 22 days, separation of the extract into acidic and neutral fractions and isolation of the cytochalasin E from the neutral fraction.
References Cited (Backward Citations)
US3457274A
Cited
US3464891A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-06-16 | 1978-12-16 | 1979-06-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-06-16 | 1982-12-16 | 1983-06-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-06-16 | 1986-12-16 | 1987-06-17 | $7700.00 | Window Closed / Lapsed |