US5264106A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enhanced amperometric sensor
Assignee: Medisense Inc
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Filing: 1993-03-18
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Granted: 1993-11-23
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Expires: 2013-03-18
Abstract
Abstract An amperometric sensor comprises a working electrode, a reference electrode, an enzyme whose catalytic activity on a substrate results in electroactivity, and an enhancer compound which enhances the detected electroactivity.
Inventors
- 👤 Jerome F. McAleer
- 👤 John T. Law
- 👤 Richard A. Morris
- 👤 Lesley Scott
- 👤 John M. Mellor
- 👤 Manus Dennison
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
89 words
An amperometric sensor for ethyl alcohol comprising a working electrode, an alcohol dehydrogenase, a reference electrode, an electron transfer compound, and a diamine compound disposed such that it reacts with a product formed by the action of said alcohol dehydrogenase but is essentially unable to react with said alcohol dehydrogenase, said alcohol dehydrogenase acting to convert alcohol to acetaldehyde and said electron transfer compound transferring electrons produced in said conversion to said working electrode, said electrodes thus developing a current indicative of the level of alcohol in a sample.
Claim 2
Dependent Claim
89 words
An amperometric sensor for ethyl alcohol comprising a working electrode, an alcohol dehydrogenase, a reference electrode, an electron transfer compound, and a diamine compound disposed such that it reacts with a product formed by the action of said alcohol dehydrogenase but is essentially unable to react with said alcohol dehydrogenase, said alcohol dehydrogenase acting to convert alcohol to acetaldehyde and said electron transfer compound transferring electrons produced in said conversion to said working electrode, said electrodes thus developing a current indicative of the level of alcohol in a sample.
Claim 3
Dependent Claim
22 words
The sensor of claim 1, wherein said diamine compound has 2 or 3 intervening carbon atoms between the nitrogens of said diamine.
Claim 4
Dependent Claim
22 words
The sensor of claim 1, wherein said diamine compound is contained in a porous layer overlaying a layer containing said alcohol dehydrogenase.
Claim 5
Dependent Claim
16 words
The sensor of claim 3, wherein said porous layer comprises said diamine compound and a surfactant.
Claim 6
Dependent Claim
13 words
The sensor of claim 3, wherein said porous layer comprises a cellulosic paper.
Claim 7
Dependent Claim
17 words
The sensor of claim 1, further comprising a protective mesh on the outer face of the sensor.
Claim 8
Dependent Claim
23 words
The sensor of claim 1, further comprising a throw-away dry strip element which comprises said electrodes and wherein said electrodes are deposited electrodes.
Claim 9
Dependent Claim
18 words
The sensor of claim 7, wherein said working electrode comprises said alcohol dehydrogenase and an electron transfer compound.
Claim 10
Dependent Claim
10 words
The sensor of claim 8, further comprising a third electrode.
Claim 11
Dependent Claim
30 words
A method of amperometrically assaying alcohol in a sample comprising supplying the sensor of claim 1, contacting a sample with said sensor, and sending the current flow from said sensor.
References Cited (Backward Citations)
USRE30267E
Cited
GB1507810A
Cited
US4476005A
Cited
DE3347104A1
Cited
US4758323A
Cited
US4795707A
Cited
US4886740A
Cited
US4897173A
Cited
US4890620A
Cited
US4830959A
Cited
US4882013A
Cited
US4704193A
Cited
US4857152A
Cited
US4919767A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-22 | 1997-05-24 | 1997-11-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-22 | 2001-05-24 | 2001-11-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-22 | 2005-05-24 | 2005-11-23 | $7700.00 | Window Closed / Lapsed |