US5256571A
Expired / Lapsed
US
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Cell preservative solution
Assignee: Cytyc Corp
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Filing: 1991-05-01
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Granted: 1993-10-26
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Expires: 2011-05-01
Abstract
Abstract An aqueous alcohol buffer solution for substantially ambient, in vitro preservation of mammalian cells for a selected duration. The solution generally contains a water-miscible alcohol in an amount sufficient to fix the sample cells without coagulation, an anti-clumping agent, and a buffer agent to maintain the solution at a pH within a range of four to seven.
Inventors
- 👤 Anne A. Hurley
- 👤 Daniel C. Lapen
- 👤 Peter S. Oud
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 01:27
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125; A01N1/126; C; C12; C12N; C12N1/00; C12N1/04; Y; Y10; Y10S; Y10S436/00; Y10S436/826; Y10T; Y10T436/00; Y10T436/10; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
71 words
An aqueous alcohol-buffer solution for maintaining the structural integrity of mammalian cells in vitro, said solution comprising A. a water-miscible alcohol in an amount sufficient to fix mammalian cells, B. an anti-clumping agent in an amount sufficient to prevent the mammalian cells from clumping in said solution, and C. a buffering agent which maintains said solution, with the mammalian cells, at a pH range of between about two to about seven.
Claim 2
Dependent Claim
71 words
An aqueous alcohol-buffer solution for maintaining the structural integrity of mammalian cells in vitro, said solution comprising A. a water-miscible alcohol in an amount sufficient to fix mammalian cells, B. an anti-clumping agent in an amount sufficient to prevent the mammalian cells from clumping in said solution, and C. a buffering agent which maintains said solution, with the mammalian cells, at a pH range of between about two to about seven.
Claim 3
Dependent Claim
19 words
The solution of claim 1 wherein said alcohol is selected from the group consisting of ethanol, isopropanol, and methanol.
Claim 4
Dependent Claim
10 words
The solution of claim 1 wherein said alcohol is methanol.
Claim 5
Dependent Claim
25 words
The solution of claim 1 wherein said anti-clumping agent is a chelating agent selected from the group consisting of ethylenediamine tetraacetic acid and salts thereof.
Claim 6
Dependent Claim
13 words
The solution of claim 1 wherein said anti-clumping agent is ethylenediamine tetraacetic acid.
Claim 7
Dependent Claim
18 words
The solution of claim 1 wherein said alcohol constitutes about 45 to about 55 percent of said solution.
Claim 8
Dependent Claim
15 words
The solution of claim 1 wherein said alcohol constitutes about 50 percent of said solution.
Claim 9
Dependent Claim
36 words
The solution of claim 1 wherein said buffering agent is selected from the group consisting of phosphate buffered saline, Tris buffer, sodium acetate, ethylenediamine tetraacetic acid, ethylenediamine tetraacetic acid salts, citric aid and citric acid salts.
Claim 10
Dependent Claim
12 words
The solution of claim 1 wherein said buffering agent is sodium acetate.
Claim 11
Dependent Claim
44 words
An aqueous alcohol-buffer solution for maintaining the structural integrity of mammalian cells in vitro, said solution comprising, by volume, A. about forty-five to fifty-five percent methanol, B. about two to four percent acetic acid, and C. about six to eight percent sodium acetate buffer.
Claim 12
Dependent Claim
44 words
An aqueous alcohol-buffer solution for maintaining the structural integrity of mammalian cells in vitro, said solution comprising, by volume, A. about forty-five to fifty-five percent methanol, B. about two to four percent acetic acid, and C. about six to eight percent sodium acetate buffer.
Claim 13
Dependent Claim
10 words
The solution of claim 10 comprising about 50 percent methanol.
Claim 14
Dependent Claim
11 words
The solution of claim 10 comprising about three percent acetic acid.
Claim 15
Dependent Claim
12 words
The solution of claim 10 comprising about seven percent sodium acetate buffer.
Claim 16
Dependent Claim
15 words
The solution of claim 1 wherein said alcohol constitutes about 20 percent of said solution.
Claim 17
Dependent Claim
24 words
The solution of claim 1 wherein said anti-clumping agent is an ethylenediamine tetraacetic acid salt selected from the group consisting of sodium and potassium.
References Cited (Backward Citations)
JPS4939437A
Cited
US4090977A
Cited
US4390632A
Cited
EP0049478A1
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GB2099281A
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US4493821A
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EP0431385A1
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-10-25 | 1997-04-26 | 1997-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-10-25 | 2001-04-26 | 2001-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-10-25 | 2005-04-26 | 2005-10-26 | $7700.00 | Window Closed / Lapsed |