US4090977A
Expired / Lapsed
US
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Osmotically balanced anticoaglant
Assignee: Research Corp
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Filing: 1976-11-22
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Granted: 1978-05-23
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Expires: 1996-11-22
Abstract
Abstract An osmotically balanced anticoagulant mixture for the preservation of whole blood which comprises an admixture of the free acid form of EDTA, which raises hematocrit values, with a corresponding amount of a salt which lowers hematocrit values, preferably an alkali metal salt of EDTA, in ratios such that the hematocrit values are comparable to those of fresh or heparinized blood over a wide range of anticoagulant concentrations.
Inventors
- 👤 Stephen Dubin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:00
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K31/00; A61K31/185; A61K31/19; A61K31/195; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/105831; Y10T436/108331
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
89 words
In a composition of matter comprising in vitro whole mammalian blood in admixture with an effective amount of an anticoagulant, the improvement wherein the anticoagulant is an osmotically balanced composition consisting essentially of (a) H 4 EDTA in free acid form and (b) at least one salt which lowers hematocrit values as determined by centrifugation, the ratio of (a) : (b) being such that said hematocrit values of the blood are substantially identical to those of fresh or heparinized blood and substantially independent of the concentration of said composition.
Claim 2
Dependent Claim
89 words
In a composition of matter comprising in vitro whole mammalian blood in admixture with an effective amount of an anticoagulant, the improvement wherein the anticoagulant is an osmotically balanced composition consisting essentially of (a) H 4 EDTA in free acid form and (b) at least one salt which lowers hematocrit values as determined by centrifugation, the ratio of (a) : (b) being such that said hematocrit values of the blood are substantially identical to those of fresh or heparinized blood and substantially independent of the concentration of said composition.
Claim 3
Dependent Claim
17 words
A composition according to claim 1, wherein (b) is at least one alkali metal salt of EDTA.
Claim 4
Dependent Claim
20 words
A composition according to claim 2, wherein said salt is Na 2 EDTA, K 3 EDTA or a mixture thereof.
Claim 5
Dependent Claim
18 words
A composition according to claim 3, wherein the weight ratio of a : b is 1:1 to 2:20.
Claim 6
Dependent Claim
13 words
A composition according to claim 4, wherein said ratio is 1:1 to 1:20.
Claim 7
Dependent Claim
18 words
A composition according to claim 1, wherein the concentration of H 4 EDTA is 0.1 - 10 mg/ml.
Claim 8
Dependent Claim
15 words
A composition according to claim 6, wherein said concentration is about 0.5 - 5 mg/ml.
Claim 9
Dependent Claim
12 words
A composition according to claim 1, wherein said blood is human blood.
Claim 10
Dependent Claim
43 words
A composition according to claim 8, wherein the concentration of H 4 EDTA is 0.1 - 10 mg/ml; component (b) is Na 2 EDTA, K 3 EDTA or a mixture thereof; and the weight ratio of (a) : (b) is 1:1 to 1:20.
Claim 11
Dependent Claim
76 words
An osmotically balanced anticoagulant for in vitro use with whole mammalian blood, which comprises an anticoagulantingly effective amount of a balanced EDTA salt composition consisting essentially of (a) H 4 EDTA and (b) a salt which lowers hematocrit values, the molar ratio of (a) : (b) being such that the hematocrit values of the blood sample are substantially identical to those of fresh or heparinized blood and substantially independent of the concentrations of (a) and (b).
Claim 12
Dependent Claim
76 words
An osmotically balanced anticoagulant for in vitro use with whole mammalian blood, which comprises an anticoagulantingly effective amount of a balanced EDTA salt composition consisting essentially of (a) H 4 EDTA and (b) a salt which lowers hematocrit values, the molar ratio of (a) : (b) being such that the hematocrit values of the blood sample are substantially identical to those of fresh or heparinized blood and substantially independent of the concentrations of (a) and (b).
Claim 13
Dependent Claim
40 words
In a method for collecting a blood sample to be used in hematological testing which comprises drawing a whole blood sample into a collection tube containing an anticoagulant, the improvement wherein said anticoagulant is the composition according to claim 10.
Claim 14
Dependent Claim
20 words
In a blood collection tube containing an anticoagulant, the improvement wherein said anticoagulant is the composition according to claim 10.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-05-22 | 1981-11-21 | 1982-05-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-05-22 | 1985-11-21 | 1986-05-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-05-22 | 1989-11-21 | 1990-05-23 | $7700.00 | Window Closed / Lapsed |