Patent US5221778A successfully refreshed from Google Patents.
US5221778A
Expired / Lapsed
US
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Multiplex gene regulation
Assignee: Yale University
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Filing: 1988-08-24
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Granted: 1993-06-22
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Expires: 2008-08-24
Abstract
Abstract A transgenic mouse offspring produced by the mating of a first transgenic mouse carrying a transresponder transgene whose expression is regulated by a viral gene product of HSV-1 and a second transgenic mouse carrying a transactivator transgene. A process for expressing a gene of interest which comprises the mating of a first transgenic mouse carrying a transresponder transgene whose expression is regulated by a viral gene product of HSV-1 and a second transgenic mouse carrying a transactivator transgene.
Inventors
- 👤 Guerard W. Byrne
- 👤 Frank H. Ruddle
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 00:51
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; C; C07; C07K; C07K14/00; C07K14/005; A01K2217/00; A01K2217/05; C12; C12N; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16611; C12N2710/16622; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
39 words
A transgenic mouse that carries in the genome of its somatic and germ cells a transresponder transgene which comprises a gene of interest operably linked to a promoter sequence which is regulated by a viral gene product of HSV-1.
Claim 2
Dependent Claim
39 words
A transgenic mouse that carries in the genome of its somatic and germ cells a transresponder transgene which comprises a gene of interest operably linked to a promoter sequence which is regulated by a viral gene product of HSV-1.
Claim 3
Dependent Claim
20 words
A transgenic mouse according to claim 1, wherein said viral gene product is encoded by the TIF gene of HSV-1.
Claim 4
Dependent Claim
20 words
A transgenic mouse according to claim 1, wherein the promoter for the transresponder transgene comprises an IE promoter of HSV-1.
Claim 5
Dependent Claim
16 words
A transgenic mouse according to claim 2, wherein said gene of interest is the CAT gene.
Claim 6
Dependent Claim
30 words
A transgenic mouse that carries in the genome of its somatic and germ cells a transactivator transgene which comprises the TIF gene of HSV-1, operably linked to a promoter sequence.
Claim 7
Dependent Claim
30 words
A transgenic mouse that carries in the genome of its somatic and germ cells a transactivator transgene which comprises the TIF gene of HSV-1, operably linked to a promoter sequence.
Claim 8
Dependent Claim
19 words
A transgenic mouse according to claim 5, wherein the promoter for the transactivator transgene comprises a mouse NF-L promoter.
Claim 9
Dependent Claim
90 words
A process for expressing a gene of interest comprising: a. introducing into a first mouse a transresponder transgene containing the gene of interest operably linked to a promoter sequence which is regulated by a viral gene product of HSV-1, b. introducing into a second mouse a transactivator transgene comprising the TIF gene of HSV-1, c. mating the first and second mice so as to produce an offspring carrying the transresponder transgene and the transactivator transgene, and maintaining said offspring under conditions effective for the expression of said gene of interest
Claim 10
Dependent Claim
90 words
A process for expressing a gene of interest comprising: a. introducing into a first mouse a transresponder transgene containing the gene of interest operably linked to a promoter sequence which is regulated by a viral gene product of HSV-1, b. introducing into a second mouse a transactivator transgene comprising the TIF gene of HSV-1, c. mating the first and second mice so as to produce an offspring carrying the transresponder transgene and the transactivator transgene, and maintaining said offspring under conditions effective for the expression of said gene of interest
Claim 11
Dependent Claim
19 words
A process according to claim 7, wherein the transresponder transgene comprises a HSV-IE promoter fused to the CAT gene.
Claim 12
Dependent Claim
22 words
A process according to claim 7, wherein the transactivator transgene comprises the mouse NF-L promoter fused to the TIF gene of HSV-1.
Claim 13
Dependent Claim
66 words
A process for expressing a gene of interest comprising: a. introducing into a mouse a transresponder transgene containing a gene of interest operably linked to a promoter sequence which is regulated by a viral gene product of HSV-1, b. infecting said mouse with a HSV-1 virus, thereby activating the transresponder transgene and maintaining said mouse under conditions effective for the expression of said gene of interest.
Claim 14
Dependent Claim
66 words
A process for expressing a gene of interest comprising: a. introducing into a mouse a transresponder transgene containing a gene of interest operably linked to a promoter sequence which is regulated by a viral gene product of HSV-1, b. infecting said mouse with a HSV-1 virus, thereby activating the transresponder transgene and maintaining said mouse under conditions effective for the expression of said gene of interest.
Claim 15
Dependent Claim
19 words
A process according to claim 10 wherein said viral gene product is encoded by the TIF gene of HSV-1.
Claim 16
Dependent Claim
15 words
A process according to claim 10, wherein said gene of interest is the CAT gene.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-06-21 | 1996-12-21 | 1997-06-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-06-21 | 2000-12-21 | 2001-06-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-06-21 | 2004-12-21 | 2005-06-22 | $7700.00 | Window Closed / Lapsed |