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US5219739A
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DNA sequences encoding bVEGF120 and hVEGF121 and methods for the production of bovine and human vascular endothelial cell growth factors, bVEGF120 and hVEGF121
Assignee: Scios LLC
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Filing: 1990-07-27
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Granted: 1993-06-15
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Expires: 2010-07-27
Abstract
Abstract Isolated DNA sequences, expression vectors and transformant cells are provided which allow for the large scale production of vascular endothelial cell growth factor. The vascular endothelial cell growth factor is useful in the treatment of wounds in which neovascularization or reendothelialization is required for healing.
Inventors
- 👤 Edmund G. Tischer
- 👤 Judith A. Abraham
- 👤 John C. Fiddes
- 👤 Richard L. Mitchell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 09:25
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/49; C07K14/46; C07K14/47; C07K14/52; C07K16/00; C07K16/18; C07K16/24; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/036; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
35 words
An isolated DNA sequence which encodes bovine vascular endothelial cell growth factor, bVEGF 120 , consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 120 of FIG. 6.
Claim 2
Dependent Claim
35 words
An isolated DNA sequence which encodes bovine vascular endothelial cell growth factor, bVEGF 120 , consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 120 of FIG. 6.
Claim 3
Dependent Claim
25 words
An isolated DNA sequence of claim 1 consisting essentially of nucleotides 1 to 341 followed by nucleotides 474 to 492 as shown in FIG. 6.
Claim 4
Dependent Claim
35 words
An isolated DNA sequence which encodes human vascular endothelial cell growth factor, hVEGF 121 , consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 121 of FIG. 7.
Claim 5
Dependent Claim
35 words
An isolated DNA sequence which encodes human vascular endothelial cell growth factor, hVEGF 121 , consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 121 of FIG. 7.
Claim 6
Dependent Claim
28 words
An isolated DNA sequence of claim 3 consisting essentially of the sequence of nucleotides 1 to 344 followed by nucleotides 477 to 495 as shown in FIG. 7.
Claim 7
Dependent Claim
49 words
An expression vector comprising a DNA sequence encoding bVEGF 120 , consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 120 of FIG. 6, operably linked to a regulatory sequence capable of directing expression of the DNA sequence in a host cell.
Claim 8
Dependent Claim
49 words
An expression vector comprising a DNA sequence encoding bVEGF 120 , consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 120 of FIG. 6, operably linked to a regulatory sequence capable of directing expression of the DNA sequence in a host cell.
Claim 9
Dependent Claim
49 words
An expression vector comprising a DNA sequence encoding hVEGF 121 , consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 121 of FIG. 7, operably linked to a regulatory sequence capable of directing expression of the DNA sequence in a host cell.
Claim 10
Dependent Claim
49 words
An expression vector comprising a DNA sequence encoding hVEGF 121 , consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 121 of FIG. 7, operably linked to a regulatory sequence capable of directing expression of the DNA sequence in a host cell.
Claim 11
Dependent Claim
33 words
An expression vector of claim 6 in which the DNA coding sequence consists essentially of the sequence of nucleotides 1 to 344 followed by nucleotides 477 to 495 as shown in FIG. 7.
Claim 12
Independent Claim
72 words
A method of producing vascular endothelial cell growth factor which comprises: culturing mammalian cells transformed with an expression vector containing a DNA sequence consisting essentially of a DNA sequence which encodes the amino acid sequence encoded by exons I-V and VIII of FIG. 8 under conditions in which the encoded polypeptide is expressed and mature hVEGF 121 is secreted from the cells; and recovering the mature hVEGF 121 from the culture medium.
Claim 13
Independent Claim
72 words
A method of producing vascular endothelial cell growth factor which comprises: culturing mammalian cells transformed with an expression vector containing a DNA sequence consisting essentially of a DNA sequence which encodes the amino acid sequence encoded by exons I-V and VIII of FIG. 8 under conditions in which the encoded polypeptide is expressed and mature hVEGF 121 is secreted from the cells; and recovering the mature hVEGF 121 from the culture medium.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-06-14 | 1996-12-14 | 1997-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-06-14 | 2000-12-14 | 2001-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-06-14 | 2004-12-14 | 2005-06-15 | $7700.00 | Window Closed / Lapsed |