Patent US4560990A successfully refreshed from Google Patents.
EP0288307A2
Expired / Lapsed
EP
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Recombinant production of PDGF A-chain polypeptides
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1988-04-22
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Granted: —
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Expires: 2008-04-22
Abstract
Abstract DNA encoding two forms of PDGF A-chain polypeptide, the construction of expression vectors for expressing such DNA in yeast and mammalian cells, and the expression of such DNA in yeast and mammalian cells to produce active PDGF A-chain homodimer and active PDGF A-chain/B-chain heterodimer are disclosed.
Inventors
- 👤 Carl-Henrik Heldin
- 👤 Christer Betsholtz
- 👤 Bengt Westermark
- 👤 Timothy J. Knott
- 👤 James c/o MRC Clinical Research Centre Scott
- 👤 Graeme I. Bell
- 👤 Leslie Rall
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 05:18
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0089; A; A61; A61P; A61P17/00; A61P43/00; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/49; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12N15/85; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/50; C07K2319/70; C07K2319/74; C07K2319/75; C12N2830/00; C12N2830/001; C12N2830/002; C12N2830/15; C12N2830/70; C12N2830/702
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
22 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence encoding a human platelet-derived growth factor (PDGF) A-chain polypeptide.
Claim 2
Dependent Claim
22 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence encoding a human platelet-derived growth factor (PDGF) A-chain polypeptide.
Claim 3
Dependent Claim
32 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 193, inclusive, in Figure 1.
Claim 4
Dependent Claim
32 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 193, inclusive, in Figure 1.
Claim 5
Dependent Claim
35 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 196, inclusive, in Figure 1 or Figure 2.
Claim 6
Dependent Claim
35 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 196, inclusive, in Figure 1 or Figure 2.
Claim 7
Dependent Claim
32 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 211, inclusive, in Figure 1.
Claim 8
Dependent Claim
32 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 211, inclusive, in Figure 1.
Claim 9
Dependent Claim
35 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain precursor polypeptide comprising the amino acid sequence numbered 1 to 196 in Figure 1 or Figure 2.
Claim 10
Dependent Claim
35 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain precursor polypeptide comprising the amino acid sequence numbered 1 to 196 in Figure 1 or Figure 2.
Claim 11
Dependent Claim
32 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain precursor polypeptide comprising the amino acid sequence numbered 1 to 211 in Figure 1.
Claim 12
Dependent Claim
32 words
A recombinant expression vector containing and effective in expressing a recombinant DNA sequence which encodes a PDGF A-chain precursor polypeptide comprising the amino acid sequence numbered 1 to 211 in Figure 1.
Claim 13
Dependent Claim
31 words
A recombinant expression vector containing and effective in expressing (a) a recombinant DNA sequence encoding a human platelet-derived growth factor (PDGF) A-chain polypeptide and (b) a DNA sequence encoding PDGF B-chain.
Claim 14
Dependent Claim
31 words
A recombinant expression vector containing and effective in expressing (a) a recombinant DNA sequence encoding a human platelet-derived growth factor (PDGF) A-chain polypeptide and (b) a DNA sequence encoding PDGF B-chain.
Claim 15
Dependent Claim
26 words
The recombinant expression vector of any one of the preceding claims wherein the DNA sequence(s) is/are operably linked to control sequences compatible with a yeast host.
Claim 16
Dependent Claim
21 words
The recombinant expression vector of claim 8 wherein said control sequences direct secretion of PDGF formed from the PDGF A-chain polypeptide.
Claim 17
Dependent Claim
27 words
The recombinant expression vector of any one of claims 1 to 7 wherein the DNA sequence(s) is/are operably linked to control sequences compatible with a mammalian host.
Claim 18
Dependent Claim
13 words
Yeast cells transformed with the recombinant expression vector of claim 8 or 9.
Claim 19
Dependent Claim
11 words
Mammalian cells transformed with the recombinant expression vector of claim 10.
Claim 20
Dependent Claim
24 words
The mammalian cells of claim 12 which are transformed with an expression vector containing and effective in expressing a DNA sequence encoding PDGF B-chain.
Claim 21
Dependent Claim
19 words
A method of producing recombinant PDGF comprised of PDGF A-chain polypeptide comprising growing the yeast cells of claim 11.
Claim 22
Dependent Claim
19 words
A method of producing recombinant PDGF comprised of PDGF A-chain polypeptide comprising growing the mammalian cells of claim 12.
Claim 23
Dependent Claim
35 words
Recombinant PDGF comprised of a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 193, inclusive, of Figure 1, or an analog of said sequence that is substantially homologous and functionally equivalent thereto.
Claim 24
Dependent Claim
35 words
Recombinant PDGF comprised of a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 193, inclusive, of Figure 1, or an analog of said sequence that is substantially homologous and functionally equivalent thereto.
Claim 25
Dependent Claim
51 words
Recombinant PDGF comprised of a PDGF A-chain polypeptide comprising (a) the amino acid sequence numbered 87 to 196, inclusive, of Figure 1, (b) the amino acid sequence numbered 87 to 196, inclusive, of Figure 2 or (c) an analog of (a) or (b) that is substantially homologous and functionally equivalent thereto.
Claim 26
Dependent Claim
51 words
Recombinant PDGF comprised of a PDGF A-chain polypeptide comprising (a) the amino acid sequence numbered 87 to 196, inclusive, of Figure 1, (b) the amino acid sequence numbered 87 to 196, inclusive, of Figure 2 or (c) an analog of (a) or (b) that is substantially homologous and functionally equivalent thereto.
Claim 27
Dependent Claim
35 words
Recombinant PDGF comprised of a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 211, inclusive, of Figure 1, or an analog of said sequence that is substantially homologous and functionally equivalent thereto.
Claim 28
Dependent Claim
35 words
Recombinant PDGF comprised of a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 211, inclusive, of Figure 1, or an analog of said sequence that is substantially homologous and functionally equivalent thereto.
Claim 29
Dependent Claim
41 words
Recombinant PDGF comprised of (a) a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 193, inclusive, of Figure 1, or an analog of said sequence that is substantially homologous and functionally equivalent thereto, and (b) a PDGF B-chain.
Claim 30
Dependent Claim
41 words
Recombinant PDGF comprised of (a) a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 193, inclusive, of Figure 1, or an analog of said sequence that is substantially homologous and functionally equivalent thereto, and (b) a PDGF B-chain.
Claim 31
Dependent Claim
44 words
Recombinant PDGF comprised of (a) a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 196, inclusive, of Figure 1 or Figure 2, or an analog of said sequence that is substantially homologous and functionally equivalent thereto, and (b) a PDGF B-chain.
Claim 32
Dependent Claim
44 words
Recombinant PDGF comprised of (a) a PDGF A-chain polypeptide comprising the amino acid sequence numbered 87 to 196, inclusive, of Figure 1 or Figure 2, or an analog of said sequence that is substantially homologous and functionally equivalent thereto, and (b) a PDGF B-chain.
Claim 33
Dependent Claim
32 words
A pharmaceutical composition for topical application to a wound comprising an effective amount of the recombinant PDGF of any one of claims 16 to 20 admixed with a topical carrier or vehicle.
References Cited (Backward Citations)
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