US4721672A
Expired / Lapsed
US
⏱️ 0.0 years remaining
CDNA and gene for human angiogenin (angiogenesis factor) and methods of expression
Assignee: Harvard University
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Filing: 1985-09-20
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Granted: 1988-01-26
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Expires: 2005-09-20
Abstract
Abstract DNA sequences encoding a protein having angiogenic activity are disclosed. Expression vectors containing these sequences are introduced into host cells and direct the production of the angiogenic protein. Proteins produced according to the invention are useful in the diagnosis of malignancies, for promoting wound healing, and for other diagnositic and therapeutic purposes.
Inventors
- 👤 Bert L. Vallee
- 👤 Kotoku Kurachi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:23
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/515; A; A61; A61P; A61P17/00; A61P43/00; A61K; A61K38/00
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
8 words
A plasmid identical to ATCC Deposit No. 40192.
Claim 2
Dependent Claim
8 words
A plasmid identical to ATCC Deposit No. 40192.
Claim 3
Dependent Claim
43 words
A purified isolated DNA sequence consisting essentially of a DNA sequence coding for the protein defined in FIG. 1 or for a protein having substantially the same amino acid sequence and substantially the same angiogenic activity as the protein defined in FIG. 1.
Claim 4
Dependent Claim
43 words
A purified isolated DNA sequence consisting essentially of a DNA sequence coding for the protein defined in FIG. 1 or for a protein having substantially the same amino acid sequence and substantially the same angiogenic activity as the protein defined in FIG. 1.
Claim 5
Dependent Claim
8 words
A plasmid identical to ATCC Deposit No. 40193.
Claim 6
Dependent Claim
8 words
A plasmid identical to ATCC Deposit No. 40193.
Claim 7
Dependent Claim
66 words
An isolated vector for expressing in transformed yeast the protein defined in FIG. 1 or a protein having substantially the same amino acid sequence and substantially the same angiogenic activity as the protein of FIG. 1, which vector includes a DNA construct comprising the DNA sequence claimed in claim 2, a promoter upstream of and operably linked to said sequence, and a polyadenylation signal downstream thereof.
Claim 8
Dependent Claim
16 words
A vector as claimed in claim 4 in which said promoter is the yeast ADHII promoter.
Claim 9
Dependent Claim
68 words
An isolated vector for expressing in a transformed mammalian cell the protein defined in FIG. 1 or a protein having substantially the same amino acid sequence and substantially the same angiogenic activity as the protein of FIG. 1, which vector includes a DNA construct comprising the DNA sequence claimed in claim 2, a promoter upstream of and operably linked to said sequence, and a polyadenylation signal downstream thereof.
Claim 10
Dependent Claim
16 words
A vector as claimed in claim 6 in which said promoter is the mouse metallothionein-1 promoter.
Claim 11
Dependent Claim
53 words
A yeast cell containing the DNA sequence coding for the protein defined in FIG. 1 or a protein having substantially the same amino acid sequence and substantially the same angiogenic activity as the protein of FIG. 1, a promoter upstream of and operably linked to said sequence, and a polyadenylation signal downstream thereof.
Claim 12
Dependent Claim
53 words
A yeast cell containing the DNA sequence coding for the protein defined in FIG. 1 or a protein having substantially the same amino acid sequence and substantially the same angiogenic activity as the protein of FIG. 1, a promoter upstream of and operably linked to said sequence, and a polyadenylation signal downstream thereof.
Claim 13
Dependent Claim
83 words
A method for producing the protein defined in FIG. 1 or a protein having substantially the same amino acid sequence and substantially the same angiogenic activity as the protein of FIG. 1, comprising the steps of inserting into cells a DNA vector having a construct comprising the DNA sequence claimed in claim 2, a promoter upstream of and operably linked to said sequence, and a polyadenylation signal downstream thereof, growing said cells in a suitable medium, and separating said protein from said cells.
Claim 14
Dependent Claim
14 words
A method as claimed in claim 9 wherein said cell is a yeast cell.
Claim 15
Dependent Claim
14 words
A method as claimed in claim 9 wherein said cell is a mammalian cell.
Claim 16
Dependent Claim
14 words
A method as claimed in claim 9 wherein said cell is a bacterial cell.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-01-25 | 1991-07-27 | 1992-01-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-01-25 | 1995-07-27 | 1996-01-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-01-25 | 1999-07-27 | 2000-01-26 | $7700.00 | Window Closed / Lapsed |