US4503038A
Expired / Lapsed
US
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Extracellular nonmitogenic angiogenesis factor and method of isolation thereof from wound fluid
Assignee: University of California, San Diego
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Filing: 1983-02-25
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Granted: 1985-03-05
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Expires: 2003-02-25
Abstract
Abstract A nonmitogenic angiogenesis factor is isolated from wound fluid by dialysis to include materials in the molecular size range of 2,000 to 14,000, lyophilization, and chromatography. The nonmitogenic angiogenesis factor is identified by activity by corneal implant assay and by cell migration assay. The angiogenesis factor is also characterized by inactivity by mitogenesis assay.
Inventors
- 👤 Michael J. Banda
- 👤 Zena Werb
- 👤 David R. Knighton
- 👤 Thomas K. Hunt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 18:02
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/515; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
115 words
A method for isolating a nonmitogenic angiogenesis factor from rabbit wound fluid accumulated at the site of damaged tissue during the process of healing comprising the steps of (a) clarifying said fluid by centrifugation; (b) dialyzing the clarified fluid from step (a) with acetic acid at about 4° C.; (c) removing insoluble material from the retentate from step (b) by centrifugation; (d) dialyzing the clarified retentate from step (c) with water at about 4° C.; (e) lyophilizing the dialysate from step (d); (f) dissolving the product from step (e) in acetic acid; (g) fractionating the solution from step (f) by size exclusion chromatography; and (h) identifying a fraction from step (g) which exhibits angiogenic activity.
Claim 2
Independent Claim
115 words
A method for isolating a nonmitogenic angiogenesis factor from rabbit wound fluid accumulated at the site of damaged tissue during the process of healing comprising the steps of (a) clarifying said fluid by centrifugation; (b) dialyzing the clarified fluid from step (a) with acetic acid at about 4° C.; (c) removing insoluble material from the retentate from step (b) by centrifugation; (d) dialyzing the clarified retentate from step (c) with water at about 4° C.; (e) lyophilizing the dialysate from step (d); (f) dissolving the product from step (e) in acetic acid; (g) fractionating the solution from step (f) by size exclusion chromatography; and (h) identifying a fraction from step (g) which exhibits angiogenic activity.
Claim 3
Dependent Claim
24 words
The method according to claim 1 wherein said step (b) is conducted in a M r 2,000 limit dialysis bag with 0.1N acetic acid.
Claim 4
Dependent Claim
22 words
The method according to claim 1 wherein said step (d) is conducted in a M r 14,000 limit dialysis bag with water.
Claim 5
Dependent Claim
17 words
The method according to claim 1 wherein said step (g) is performed by high pressure liquid chromatography.
Claim 6
Dependent Claim
16 words
The method according to claim 1 wherein said step (h) is conducted by corneal implant assay.
Claim 7
Dependent Claim
19 words
The product comprising nonmitogenic angiogenesis factor produced according to the method of claims 1, 2, 3, 4 or 5.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-03-04 | 1988-09-03 | 1989-03-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-03-04 | 1992-09-03 | 1993-03-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-03-04 | 1996-09-03 | 1997-03-05 | $7700.00 | Window Closed / Lapsed |