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Patent US5215889A successfully refreshed from Google Patents.
US5215889A
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Catalytic and reactive polypeptides and methods for their preparation and use
Assignee: University of California
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Filing: 1989-09-08
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Granted: 1993-06-01
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Expires: 2009-09-08
Abstract
Abstract Catalytic and reactive polypeptides include a binding site specific for a reactant or reactive intermediate involved in a chemical reaction of interest. The polypeptides further include at least one active functionality proximate the binding site, where the active functionality is capable of catalyzing or chemically participating in the chemical reaction in such a way that the reaction rate is enhanced. Methods for preparing the catalytic peptides include chemical synthesis, site-directed mutagenesis of antibody and enzyme genes, covalent attachment of the functionalities through particular amino acid side chains, and the like.
Inventors
- 👤 Peter Schultz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:50
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/655; C12; C12N; C12N9/00; C12N9/14; C12N9/78; C12N9/86; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6835; C07K16/00; C12N15/00; C12N15/09; C12N15/10; C12N9/0002
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
124 words
A polypeptide capable of promoting a chemical reaction involving the conversion of one or more reactants to one or more products, said polypeptide having a binding site specific for at last one reactant or reaction intermediate, wherein the polypeptide is other than a naturally occurring enzyme and includes at least one active functionality covalently attached to the polypeptide proximate the binding site, which functionality is capable of chemically participating in the chemical reaction or catalytically enhancing the chemical reaction rate, wherein the functionality is either a moiety covalently attached to an amino acid side chain proximate the binding site or an amino acid side chain other than a naturally occurring amino acid side chain and wherein the polypeptide is an antibody or antibody fragment.
Claim 2
Dependent Claim
124 words
A polypeptide capable of promoting a chemical reaction involving the conversion of one or more reactants to one or more products, said polypeptide having a binding site specific for at last one reactant or reaction intermediate, wherein the polypeptide is other than a naturally occurring enzyme and includes at least one active functionality covalently attached to the polypeptide proximate the binding site, which functionality is capable of chemically participating in the chemical reaction or catalytically enhancing the chemical reaction rate, wherein the functionality is either a moiety covalently attached to an amino acid side chain proximate the binding site or an amino acid side chain other than a naturally occurring amino acid side chain and wherein the polypeptide is an antibody or antibody fragment.
Claim 3
Dependent Claim
23 words
A polypeptide as in claim 1, wherein the distance between the active functionality and the binding site is less than about 20 Å.
Claim 4
Dependent Claim
40 words
A polypeptide as in claim 1, wherein the active functionality is capable of catalytically enhancing the reaction rate and is selected from the group consisting of acids, bases, enzyme cofactors, metal complexes, photoactive molecules, nucleophiles, electrophiles, and redox active molecules.
Claim 5
Dependent Claim
38 words
A polypeptide as in claim 1, wherein the active functionality is capable of chemically participating in the chemical reaction and is selected from the group consisting of alkylating agents, oxidizing agents, reducing agents, hydrolytic agents, and photoactive agents.
Claim 6
Dependent Claim
66 words
A polypeptide having a binding site and an active functionality covalently attached to a side chain of an amino acid proximate the binding site, wherein the binding site is capable of complexing a reactant or reaction intermediate in a chemical reaction and the active functionality is capable of enhancing the rate of a chemical reaction, and wherein the polypeptide is an antibody or an antibody fragment.
Claim 7
Dependent Claim
66 words
A polypeptide having a binding site and an active functionality covalently attached to a side chain of an amino acid proximate the binding site, wherein the binding site is capable of complexing a reactant or reaction intermediate in a chemical reaction and the active functionality is capable of enhancing the rate of a chemical reaction, and wherein the polypeptide is an antibody or an antibody fragment.
Claim 8
Dependent Claim
40 words
A polypeptide as in claim 5, wherein the active functionality is capable of catalytically enhancing the reaction rate and is selected from the group consisting of acids, bases, enzyme cofactors, metal complexes, photoactive molecules, nucleophiles, electrophiles, and redox active molecules.
Claim 9
Dependent Claim
31 words
A polypeptide as in claim 6, wherein the active functionality is an enzyme cofactor or derivative thereof selected from the group consisting of flavins, porphyrins, thiamine, pyridoxamine, nicotinamide, biotin, and folates.
Claim 10
Dependent Claim
38 words
A polypeptide as in claim 6, wherein the active functionality is a metal complex selected from the group consisting of chelated Lewis acids, redox active metal complexes, photoactive metal complexes, oxygen activating metal complexes, and radioactive metal complexes.
Claim 11
Dependent Claim
41 words
A polypeptide as in claim 5, wherein the active functionality is capable of chemically participating in the chemical reaction and is selected from the group consisting of alkylating agents, oxidizing agents, reducing agents, hydrolytic agents, photosensitizers, electrophiles, nucleophiles, acids, and bases.
Claim 12
Dependent Claim
19 words
A polypeptide as in claim 5, which is a modification of the F(ab) or Fv fragment of an antibody.
Claim 13
Dependent Claim
28 words
A polypeptide as in claim 5, wherein the active functionality is covalently attached to a side chain of an amino acid other than a naturally occurring amino acid.
Claim 14
Independent Claim
114 words
A method for enhancing the rate of a chemical reaction involving the conversion of one or more reactants to one or more products, said method comprising exposing a reaction mixture including said reactant(s) to a polypeptide selected from the group consisting of antibodies and antibody fragments and having a binding site capable of forming a complex with said reactant(s) or a reaction intermediate including said reactant(s), wherein an active functionality is a side chain of a non-natural amino acid proximate the binding site or is a moiety covalently attached to a side chain of an amino acid proximate the binding site, said active functionality being capable of promoting the conversion of reactant(s) to product(s).
Claim 15
Independent Claim
114 words
A method for enhancing the rate of a chemical reaction involving the conversion of one or more reactants to one or more products, said method comprising exposing a reaction mixture including said reactant(s) to a polypeptide selected from the group consisting of antibodies and antibody fragments and having a binding site capable of forming a complex with said reactant(s) or a reaction intermediate including said reactant(s), wherein an active functionality is a side chain of a non-natural amino acid proximate the binding site or is a moiety covalently attached to a side chain of an amino acid proximate the binding site, said active functionality being capable of promoting the conversion of reactant(s) to product(s).
Claim 16
Dependent Claim
34 words
A method as in claim 12, wherein the active functionality is a catalytic functionality selected from the group consisting of acids, bases, enzyme cofactors, metal complexes, photoactive molecules, nucleophiles, electrophiles, and redox active molecules.
Claim 17
Dependent Claim
31 words
A method as in claim 13, wherein the catalytic functionality is an enzyme cofactor or derivative thereof selected from the group consisting of flavine, porphyrins, thiamine, pyridoxamine, nicotinamide, biotin, and folates.
Claim 18
Dependent Claim
38 words
A method as in claim 13, wherein the catalytic functionality is a metal complex selected from the group consisting of chelated Lewis acids, redox active metal complexes, photoactive metal complexes, oxygen activating metal complexes, and radioactive metal complexes.
Claim 19
Dependent Claim
34 words
A method as in claim 12, wherein the active functionality is a reactive functionality selected from the group consisting of alkylating agents, oxidizing agents, reducing agents, hydrolytic agents, photosensitizers, electrophiles, nucleophiles, acids, and bases.
Claim 20
Dependent Claim
30 words
A method as in claim 12, wherein the antibody has been prepared against a hapten which is a reactant, a reactant analog, a transition state analog, or a multisubstrate analog.
Claim 21
Dependent Claim
20 words
A method as in claim 12, wherein the reaction is ester hydrolysis and the active functionality is an imidazole derivative.
Claim 22
Dependent Claim
18 words
A method as in claim 12, wherein the reaction is thiolysis and the active functionality is alkyl mercaptan.
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