US4792446A
Expired / Lapsed
US
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Production of antibody catalysts
Assignee: IGEN Inc A CORP OF
•
Filing: 1986-06-23
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Granted: 1988-12-20
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Expires: 2006-06-23
Abstract
Abstract Process for producing an antibody catalyst for a chemical reaction. Chemical reactions in which the antibody catalyst can be used are also disclosed.
Inventors
- 👤 Peter S. Kim
- 👤 Neville R. Kallenbach
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 21:03
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0002; Y; Y10; Y10S; Y10S530/00; Y10S530/808
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
212 words
Process for producing an antibody catalyst for a chemical reaction, which comprises: (1) identifying a substrate represented by formula R.sub.1 --X--R.sub.2 for said reaction; (2) selecting a hapten represented by formula ##STR26## wherein X represents nuclei O, S, N, and C and associated bonds which comprise the catalytically active portion of the substrate molecule and Y represents nuclei N, C, and P and associated bonds which comprise the catalytically active portion of the hapten molecule and X and Y are related to each other in that Y has a higher valence state and one or more bonds than X, and wherein Q is selected from the group of substituents consisting of (a) a positive charge, (b) a negative charge, (c) polarity, (d) non-polarity, and (e) substantial bulk bonded to Y wherein R 1 , R 2 , R 1 ' and R 2 ' represent the residual chemical groups of the substrate and hapten which do not participate in the catalytic events, and R 1 and R 1 ', and R 2 and R 2 ' are, respectively substantially similar to each other which corresponds to said substrate; (3) stimulating an immune response by said hapten; and (4) isolating antibodies from said immune response which are catalytically active for said chemical reaction.
Claim 2
Dependent Claim
212 words
Process for producing an antibody catalyst for a chemical reaction, which comprises: (1) identifying a substrate represented by formula R.sub.1 --X--R.sub.2 for said reaction; (2) selecting a hapten represented by formula ##STR26## wherein X represents nuclei O, S, N, and C and associated bonds which comprise the catalytically active portion of the substrate molecule and Y represents nuclei N, C, and P and associated bonds which comprise the catalytically active portion of the hapten molecule and X and Y are related to each other in that Y has a higher valence state and one or more bonds than X, and wherein Q is selected from the group of substituents consisting of (a) a positive charge, (b) a negative charge, (c) polarity, (d) non-polarity, and (e) substantial bulk bonded to Y wherein R 1 , R 2 , R 1 ' and R 2 ' represent the residual chemical groups of the substrate and hapten which do not participate in the catalytic events, and R 1 and R 1 ', and R 2 and R 2 ' are, respectively substantially similar to each other which corresponds to said substrate; (3) stimulating an immune response by said hapten; and (4) isolating antibodies from said immune response which are catalytically active for said chemical reaction.
Claim 3
Dependent Claim
14 words
Process of claim 1 wherein X is O or S and Y is N.
Claim 4
Dependent Claim
12 words
Process of claim 1 wherein X is N and Y is C.
Claim 5
Dependent Claim
12 words
Process of claim 1 wherein X is C and Y is P.
Claim 6
Dependent Claim
13 words
Process of claim 1 wherein Q is a substituent comprising a positive charge.
Claim 7
Dependent Claim
23 words
Process of claim 5 wherein Q is a substituent selected from the group comprising --NH 3 .sup.(+), --NR 2 H.sup.(+), --NR 3 .sup.(+).
Claim 8
Dependent Claim
13 words
Process of claim 1 wherein Q is a substituent comprising a negative charge.
Claim 9
Dependent Claim
24 words
Process of claim 7 wherein Q is a substituent selected from the group comprising CO 2 .sup.(-), PO 4 .sup.(-2) and SO 4 .sup.(-2).
Claim 10
Dependent Claim
11 words
Process of claim 1 wherein Q is a substituent comprising polarity.
Claim 11
Dependent Claim
28 words
Process of claim 9 wherein Q is a substituent selected from the group comprising --NO 2 , --C--NH 2 , --N--(CH 2 F) 2 , --CL and --SH.
Claim 12
Dependent Claim
11 words
Process of claim 1 wherein Q is a substituent comprising non-polarity.
Claim 13
Dependent Claim
19 words
Process of claim 11 wherein Q is a substituent selected from the group comprising ##STR27## and --CH 3 .
Claim 14
Dependent Claim
12 words
Process of claim 1 wherein Q is a substituent comprising substantial bulk.
Claim 15
Dependent Claim
26 words
Process of claim 13 wherein Q is a substituent selected from the group comprising --CO 2 , --NH 2 , --NH 3 and --NO 2 .
Claim 16
Dependent Claim
11 words
Process of claim 1 wherein the immune response is stimulated monoclonally.
Claim 17
Dependent Claim
11 words
Process of claim 1 wherein the immune response is stimulated polyclonally.
Claim 18
Dependent Claim
14 words
Process of claim 1 wherein the chemical reaction is a general acid-base catalysis reaction.
Claim 19
Dependent Claim
17 words
Process of claim 1 wherein the chemical reaction involves charge stabilization or destabilization in the transition state.
Claim 20
Dependent Claim
18 words
Process of claim 18 wherein the charge stabilization or destabilization in the transition state is a fragmentation reaction.
Claim 21
Dependent Claim
12 words
Process of claim 19 wherein the fragmentation reaction is a decarboxylation reaction.
Claim 22
Dependent Claim
15 words
Process of claim 17 wherein the general acid-base catalysis reaction is hydrolysis of uric acid.
Claim 23
Dependent Claim
22 words
Process of claim 17 wherein the general acid-base catalysis reaction is hydrolysis of protein molecules comprising an arbitrary sequence of amino acids.
Claim 24
Dependent Claim
20 words
Process of claim 17 wherein the general acid-base catalysis reaction is hydrolysis of polydeoxynucleotides or polyribonucleotides comprising a specific sequence.
Claim 25
Dependent Claim
25 words
Process of claim 1 wherein the catalytic antibody is purified from a resulting globulin fraction antibody active as a specific catalyst for the chemical reaction.
References Cited (Backward Citations)
WO1985002414A1
Cited
US4659567A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-12-20 | 1992-06-20 | 1992-12-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-12-20 | 1996-06-20 | 1996-12-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-12-20 | 2000-06-20 | 2000-12-20 | $7700.00 | Window Closed / Lapsed |