US4764464A
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Semisynthetic enzymes: apoproteins from heme proteins as hydrolases
Assignee: Honeywell International Inc
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Filing: 1986-03-20
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Granted: 1988-08-16
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Expires: 2006-03-20
Abstract
Abstract Proteins having a hydrophobic cavity proximate to which is a residue promoting hydrolysis of functional moieties are effective semisynthetic hydrolases. Heme proteins from which the heme has been removed usually have at least one imidazole residue proximate to the cavity, and thus act as quite effective esterases. Because the size and shape of the cavities of such proteins are capable of broad diversity a wide spectrum of substrates may be hydrolyzed by these semisynthetic enzymes.
Inventors
- 👤 Haya Zemel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:50
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/16; C12N9/18; Y; Y02; Y02E; Y02E50/00; Y02E50/10
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
44 words
A method of hydrolyzing a carboxylic ester comprising reacting a solution of said ester with water in the presence of an apoprotein whose parent is a heme protein which is myoglobin or hemoglobin in an amount effective to catalyze the hydrolysis of the ester.
Claim 2
Independent Claim
44 words
A method of hydrolyzing a carboxylic ester comprising reacting a solution of said ester with water in the presence of an apoprotein whose parent is a heme protein which is myoglobin or hemoglobin in an amount effective to catalyze the hydrolysis of the ester.
Claim 3
Dependent Claim
17 words
The method of claim 1 where the solution of the organic molecule contains a water-miscible organic cosolvent.
Claim 4
Dependent Claim
17 words
The method of claim 2 where the organic solvent is present up to about 15% by volume.
Claim 5
Dependent Claim
34 words
The method of claim 2 where the water-miscible organic solvent is selected from the group consisting of acetonitrile, dioxane, lower aliphatic alcohols, n-methylacetamide, dimethylformamide, dimethyl sulfoxide, tetrahydrofuran, hexamethylphosphoramide, ethylene glycol, propylene glycol, and glycerol.
Claim 6
Dependent Claim
12 words
The method of claim 1 where the heme protein is a hemoglobin.
Claim 7
Dependent Claim
12 words
The method of claim 1 where the heme protein is a myoglobin.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-16 | 1992-02-15 | 1992-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-16 | 1996-02-15 | 1996-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-16 | 2000-02-15 | 2000-08-16 | $7700.00 | Window Closed / Lapsed |