Patent US5215882A successfully refreshed from Google Patents.
US5215882A
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Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays
Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1989-11-30
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Granted: 1993-06-01
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Expires: 2009-11-30
Abstract
Abstract Methods of immobilizing nucleic acid on a solid surface for us in nucleic acid hybridization assays is disclosed. The methods of the invention comprise reacting a modified nucleic acid strand comprising a variable portion and an anchor portion wherein the variable portion comprises a nucleotide sequence having a selected base sequence and the anchor portion comprises at least one nucleotide base modified with a primary amine function or nucleotide base equivalent having a primary amine function and reacting the modified nucleic acid strand with a free aldehyde group of the solid surface in the presence of a reducing agent to form complexes of the modified nucleic acid strand and at least a portion of the free aldehyde groups on the solid surface.
Inventors
- 👤 Chander Bahl
- 👤 Rhonda Lang
- 👤 Leopoldo G. Mendoza
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 07:45
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
151 words
A method of immobilizing nucleic acid to a solid surface having free aldehyde groups comprising the steps of: (a) providing a modified nucleic acid strand comprising a variable portion and an anchor portion wherein said variable portion comprises a nucleotide sequence having a selected base sequence and said anchor portion comprises from two to about 10 nucleotide bases modified with a primary amine function or nucleotide base equivalents having a primary amine function; and (b) reacting the primary amine functions of said anchor portion of said modified nucleic acid strand with the free aldehyde groups of said solid surface in a preselected pattern in the presence of a reducing agent to form complexes of said modified nucleic acid strand and at least a portion of said free aldehyde groups, such that said modified nucleic acid strand is attached to said solid surface through the primary strand functions of said anchor portion.
Claim 2
Independent Claim
151 words
A method of immobilizing nucleic acid to a solid surface having free aldehyde groups comprising the steps of: (a) providing a modified nucleic acid strand comprising a variable portion and an anchor portion wherein said variable portion comprises a nucleotide sequence having a selected base sequence and said anchor portion comprises from two to about 10 nucleotide bases modified with a primary amine function or nucleotide base equivalents having a primary amine function; and (b) reacting the primary amine functions of said anchor portion of said modified nucleic acid strand with the free aldehyde groups of said solid surface in a preselected pattern in the presence of a reducing agent to form complexes of said modified nucleic acid strand and at least a portion of said free aldehyde groups, such that said modified nucleic acid strand is attached to said solid surface through the primary strand functions of said anchor portion.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said reducing agent is sodium cyanoborohydride.
Claim 4
Dependent Claim
19 words
The method of claim 1 wherein said solid surface is cellulose paper having free aldehyde groups on its surfaces.
Claim 5
Dependent Claim
22 words
The method of claim 1 wherein said anchor portion comprises from about five to about eight nucleotide bases or nucleotide base equivalents.
Claim 6
Dependent Claim
35 words
The method of claim 1 wherein said nucleotide base or nucleotide base equivalent is cytosine having nitrogen at the exocyclic 4 position of the pyrimidine ring modified with a substituent having a primary amine function.
Claim 7
Dependent Claim
20 words
The method of claim 1 wherein said variable portion is from about two to about 1,000 nucleotide bases in length.
Claim 8
Dependent Claim
20 words
The method of claim 6 wherein said variable portion is from about 15 to about 100 nucleotide bases in length.
Claim 9
Dependent Claim
17 words
The method of claim 1 wherein said variable portion of the modified nucleotide strand is a homopolymer.
Claim 10
Dependent Claim
11 words
The method of claim 8 wherein said homopolymer is poly thymidine.
Claim 11
Dependent Claim
11 words
The method of claim 8 wherein said homopolymer is poly adenine.
Claim 12
Dependent Claim
11 words
The method of claim 1 wherein said variable portion is RNA.
Claim 13
Dependent Claim
11 words
The method of claim 1 wherein said variable portion is DNA.
Claim 14
Dependent Claim
13 words
The method of claim 1 wherein said variable portion is of human origin.
Claim 15
Dependent Claim
13 words
The method of claim 1 wherein said variable portion is of bacterial origin.
Claim 16
Dependent Claim
13 words
The method of claim 1 wherein said variable portion is of viral origin.
Claim 17
Dependent Claim
13 words
The method of claim 1 wherein said variable portion is of fungal origin.
Claim 18
Dependent Claim
28 words
The method of claim 1 further comprising the step of reacting unreacted aldehyde groups from step (b) with a mixture of an amino acid and a reducing agent.
Claim 19
Dependent Claim
13 words
The method of claim 17 wherein said amino acid is α-amino caproic acid.
Claim 20
Dependent Claim
22 words
A solid surface for conducting nucleic acid hybridization assays comprising a solid surface prepared in accordance with the method of claim 1.
Claim 21
Dependent Claim
22 words
A solid surface for conducting nucleic acid hybridization assays comprising a solid surface prepared in accordance with the method of claim 8.
Claim 22
Dependent Claim
47 words
A kit for performing a nucleic hybridization assay comprising, a solid surface of claim 19 and at least one labeled nucleic acid probe, said solid surface and said at least one nucleic acid probe selected to form a detection system for a target strand of nucleic acid.
Claim 23
Dependent Claim
47 words
A kit for performing a nucleic hybridization assay comprising, a solid surface of claim 20 and at least one labeled nucleic acid probe, said solid surface and said at least one nucleic acid probe selected to form a detection system for a target strand of nucleic acid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-05-31 | 1996-11-30 | 1997-06-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-05-31 | 2000-11-30 | 2001-06-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-05-31 | 2004-11-30 | 2005-06-01 | $7700.00 | Window Closed / Lapsed |