US4423208A
Expired / Lapsed
US
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Affinity gel-adsorbent
Assignee: Individual
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Filing: 1982-02-24
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Granted: 1983-12-27
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Expires: 2002-02-24
Abstract
Abstract An improved affinity gel-adsorbent for use in an improved method of purifying glucocorticoid receptor by affinity chromatography and having the formula: ##STR1## R 1 : H or OH or ═O R 2 : H or F R 3 : H or F R 4 : OH or H R 5 : OH or H ##STR2## R 6 : H or CH 3 X: 1 through 8 M: Matrix ##STR3## Alternative A ring with Δ1,2 double bond.
Inventors
- 👤 Peter Grandics
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/743; B; B01; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/289; B01J20/30; B01J20/32; B01J20/3202; B01J20/3206; B01J20/3208; B01J20/3212; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3251; B01J20/3253; C; C07; C07J; C07J41/00; C07J41/0033; C07J41/005; C07J71/00; C07J71/0005; C07J71/0026; C07J71/0031
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
72 words
An improved affinity gel-adsorbent having the formula: ##STR11## R 1 : H or OH or═O R 2 : H or F R 3 : H or F R 4 : OH or H R 5 : OH or H ##STR12## R 6 : H or CH 3 X: 1 through 8 M: Matrix ##STR13## Alternative A ring with Δ1,2 double bond. wherein M is a polysaccharide matrix including agarose, dextran, and cellulose.
Claim 2
Dependent Claim
72 words
An improved affinity gel-adsorbent having the formula: ##STR11## R 1 : H or OH or═O R 2 : H or F R 3 : H or F R 4 : OH or H R 5 : OH or H ##STR12## R 6 : H or CH 3 X: 1 through 8 M: Matrix ##STR13## Alternative A ring with Δ1,2 double bond. wherein M is a polysaccharide matrix including agarose, dextran, and cellulose.
Claim 3
Dependent Claim
85 words
An improved process of making an affinity gel-adsorbent comprising: A. coupling, in a pH range of 7 to 9, a steroid ligand having the following formula: ##STR14## B. with an amino gel having the formula: ##STR15## C. Wherein R 1 : H or OH R 2 : H or F R 3 : H or F R 4 : OH or H R 5 : OH or H ##STR16## R 6 : H or CH 3 M: agarose, dextran, or cellulose X: 1 to 8
Claim 4
Dependent Claim
85 words
An improved process of making an affinity gel-adsorbent comprising: A. coupling, in a pH range of 7 to 9, a steroid ligand having the following formula: ##STR14## B. with an amino gel having the formula: ##STR15## C. Wherein R 1 : H or OH R 2 : H or F R 3 : H or F R 4 : OH or H R 5 : OH or H ##STR16## R 6 : H or CH 3 M: agarose, dextran, or cellulose X: 1 to 8
Claim 5
Dependent Claim
9 words
The process of claim 2 wherein A is ##STR17##
Claim 6
Dependent Claim
53 words
The process of claim 2 wherein said steroid ligand results when in first sequence C-21 corticosteroid is initially treated with methanesulfonyl in dry pyridine, and in second sequence with methyl-p-hydroxybenzoate sodium salt, and in third sequence with a molar excess of N-hydroxysuccinimide and an equimolar amount of N,N 1 -dicyclohexylcarbodiimide in peroxide-free dioxane.
Claim 7
Dependent Claim
48 words
The process of claim 2 wherein in first sequence, said amino-gel results when said polysaccharide matrice are reacted with a di-epoxide having the formula ##STR18## in an alkaline medium, and in second sequence with ammonia, and in third sequence is suspended in a mixture of dioxane and water.
Claim 8
Dependent Claim
36 words
The process of claim 2 wherein said gel-adsorbent is successively washed in first sequence with dioxane and water and 1 M sodium chloride, and then acetylated with acetic anhydride, and finally washed with dioxane and methanol.
Claim 9
Dependent Claim
31 words
The process of claim 2 wherein said gel-adsorbent is regenerated after use with 1 M sodium chloride, distilled water and an organic mixture of chloroform, methanol, distilled water and treton X-100.
References Cited (Backward Citations)
GB805830A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-12-26 | 1987-06-27 | 1987-12-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-12-26 | 1991-06-27 | 1991-12-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-12-26 | 1995-06-27 | 1995-12-27 | $7700.00 | Window Closed / Lapsed |