US5176899A
Expired / Lapsed
US
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Antimicrobial dentifrice
Assignee: REMS SYSTEMS Inc A CORP OF
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Filing: 1991-11-25
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Granted: 1993-01-05
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Expires: 2011-11-25
Abstract
Abstract A stabilized aqueous dentifrice composition capable of producing or, in the presence of saliva, leading to the production of antimicrobially effective concentrations of hypothiocyanite ions (OSCN--) are herein described. The composition contains both an oxidoreductase enzyme and its specific substrate, for the purpose of producing hydrogen peroxide of at least the minimum effective concentration. The aqueous dentifrice compositions of the present invention can be stabilized against premature enzyme/substrate interaction by controlling the level of dissolved oxygen in the aqueous dentifrice carrier. Optionally, a peroxidase enzyme may be included to act upon the aforementioned hydrogen peroxide, thereby oxidizing salivary thiocyanate ions to produce the antimicrobial concentrations of hypothiocyanite ions (OSCN--). Optionally, thiocyanate ions may also be included in the compositions of this invention in an amount sufficient, together with the other inventive ingredients, to produce in excess of about 100 micromoles/liter/minute of hypothiocyanite ions during use. The amount of water contained in dentifrice compositions is not important to the stability of the composition, provided that the level of oxygen is kept in control. The method of making the dentifrice composition to contain minimal amounts of oxygen is also disclosed.
Inventors
- 👤 Robert E. Montgomery
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 20:49
CPC Classifications
A; A61; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/64; A61K8/66; A61Q; A61Q11/00
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
120 words
An anaerobically packaged dentifrice composition with an antibacterial system comprising: a liquid carrier comprising an oxidoreductase enzyme and an oxidoreductase enzyme substrate, wherein said enzyme and substrate form hydrogen peroxide when reacted together, said hydrogen peroxide being formed at a rate of at least 100 micromoles per liter per minute; wherein said composition contains oxygen in a limited concentration such that level of dissolved oxygen in the fluid carrier cannot support substantial formation of the hydrogen peroxide, said dentifrice having been anaerobically manufactured, stored and packaged entirely in a vacuum or in an inert gas environment and having a level of dissolved oxygen at less than 3.2 parts per million and being packaged in an oxygen impervious package or container.
Claim 2
Dependent Claim
120 words
An anaerobically packaged dentifrice composition with an antibacterial system comprising: a liquid carrier comprising an oxidoreductase enzyme and an oxidoreductase enzyme substrate, wherein said enzyme and substrate form hydrogen peroxide when reacted together, said hydrogen peroxide being formed at a rate of at least 100 micromoles per liter per minute; wherein said composition contains oxygen in a limited concentration such that level of dissolved oxygen in the fluid carrier cannot support substantial formation of the hydrogen peroxide, said dentifrice having been anaerobically manufactured, stored and packaged entirely in a vacuum or in an inert gas environment and having a level of dissolved oxygen at less than 3.2 parts per million and being packaged in an oxygen impervious package or container.
Claim 3
Dependent Claim
25 words
The composition of claim 1 wherein dissolved oxygen is contained in said composition in a concentration of less than about 100 micromoles/liter of liquid carrier.
Claim 4
Dependent Claim
22 words
The composition of claim 1 wherein said hydrogen peroxide is formed at a rate in the range of 1.0 to 5.0 millimoles/liter/minute.
Claim 5
Dependent Claim
17 words
The composition of claim 1 further comprising a peroxidase enzyme for oxidizing thiocyanate ions to hypothiocyanite ions.
Claim 6
Dependent Claim
22 words
The composition of claim 1 wherein said hydrogen peroxide does not accumulate to a concentration greater than about 1.0 millimole/liter during use.
Claim 7
Dependent Claim
48 words
The composition of claim 1 wherein said oxidoreductase is selected from the group consisting of glucose oxidase, galactose oxidase, glycollate oxidase, lactate oxidase, L-gulunolactone oxidase, L-2-hydroxyacid oxidase, aldehyde oxidase, xanthine oxidase, D-aspartate oxidase, L-amino acid oxidase, D-amino acid oxidase, monoamine oxidase, pyridoxaminephosphate oxidase, diamine oxidase, and sulfite oxidase.
Claim 8
Dependent Claim
11 words
The composition of claim 6 wherein said oxidoreductase is glucose oxidase.
Claim 9
Dependent Claim
44 words
The composition of claim 1 wherein said substrates are specific to the particular oxidoreductase and are selected from D-glucose, D-galactose, L-sorbose, ethanol, tyramine, 1,4-diaminobutane, 6-hydroxy-L-nicotine, 6-hydroxy-D-nicotine, 2-aminophenol, glycollate, L-lactate, 2-deoxy-D-Glucose, L-gulunolactone, L-galactonolactone, D-mannonolactone, L-2-hydroxyisocaproate, acetaldehyde, butyraldehyde, xanthine, D-aspartate, D-glutamate, L-amino acids and D-amino acids.
Claim 10
Dependent Claim
16 words
The composition of claim 1 wherein said oxidoreductase is glucose oxidase and said substrate is beta-D-glucose.
Claim 11
Dependent Claim
18 words
The composition of claim 4 wherein the peroxidase enzyme is selected from lactoperoxidase, myeloperoxidase, salivary peroxidase, and chloroperoxidase.
Claim 12
Dependent Claim
10 words
The composition of claim 10 wherein the peroxidase is lactoperoxidase.
Claim 13
Dependent Claim
26 words
The composition of claim 4 wherein the peroxidase is provided in an amount sufficient to produce hypothiocyanite ions at a rate of at least 100 micromoles/liter/minute.
Claim 14
Dependent Claim
26 words
The composition of claim 1 further comprising thiocyanate ions at concentrations of from about 0.10 millimoles/gram of liquid carrier to about 10.00 millimoles/gram of liquid carrier.
Claim 15
Dependent Claim
26 words
The composition of claim 13 wherein said thiocyanate ion is provided by the compounds selected from sodium thiocyanate, potassium thiocyanate, ammonium thiocyanate and other thiocyanate salts.
Claim 16
Dependent Claim
17 words
The composition of claim 13 wherein said thiocyanate ion is provided by potassium thiocyanate and sodium thiocyanate.
Claim 17
Dependent Claim
30 words
The composition of claim 1, wherein said composition comprises a fluid carrier, comprised of water, in an amount ranging from about 5% to about 90% by weight of the composition.
Claim 18
Dependent Claim
24 words
The composition of claim 16 further comprising a humectant selected from glycerine, propylene glycol, sorbitol (70% solution), polyethylene glycols, polypropylene glycols, and mixtures thereof.
Claim 19
Dependent Claim
23 words
The composition of claim 16 wherein said water comprises in the range from about 10% to about 50% by weight of said composition.
Claim 20
Dependent Claim
20 words
The composition of claim 17 wherein said water comprises from about 20% to about 35% by weight of the composition.
Claim 21
Dependent Claim
49 words
The composition of claim 1 further comprising a thickener selected from natural and synthetic water-soluble polymers selected from sodium carboxymethylcellulose, xanthan gum, calcium carrageenan, locust bean gum, gum tragacanth, hydroxyethylcellulose, sodium alginate, starch, polyvinylpyrrolidone and polyacrylic acid and inorganic thickeners selected from magnesium aluminum silicate, hectorites and hydrated silicas.
Claim 22
Dependent Claim
40 words
The composition of claim 1 further comprising abrasives selected from the group consisting of calcium pyrophosphate, calcium carbonate, hydrated silica, aluminum hydroxide, dicalcium phosphate dihydrate, tricalcium phosphate, sodium metaphosphate, potassium metaphosphate, aluminum silicate, finely divided poly(methyl methacrylate), and mixtures thereof.
Claim 23
Dependent Claim
24 words
The composition of claim 21 wherein said abrasive is present in the composition in concentrations of from about 5% to about 7% by weight.
Claim 24
Dependent Claim
23 words
The composition of claim 21 wherein said abrasive is present in the composition in concentrations from about 10% to about 50% by weight.
Claim 25
Dependent Claim
21 words
The composition of claim 1 further comprising at least one surfactant selected from the group consisting of nonionic and amphoteric surfactants.
Claim 26
Dependent Claim
31 words
The composition of claim 24 wherein said surfactant is selected from the group consisting of polysorbate 80, cocoamidopropylbetaine, cocoamphopropionate, ethoxylated (20), isocetyl alcohol and ethylene oxide/propylene oxide block copolymer nonionic surfactant.
Claim 27
Dependent Claim
16 words
The composition of claim 1 having a pH in the range of approximately 5.5 to 7.5.
Claim 28
Dependent Claim
14 words
The composition of claim 26 wherein said composition further comprises a physiologically acceptable buffer.
Claim 29
Dependent Claim
24 words
The composition of claim 27 wherein said physiologically acceptable buffer is selected from potassium phosphate, sodium phosphate, disodium phosphate, dipotassium phosphate, and mixtures thereof.
Claim 30
Dependent Claim
24 words
The composition of claim 27 wherein said buffer is in a concentration from about 0.01 moles to about 0.50 moles/liter of fluid dentifrice carrier.
Claim 31
Dependent Claim
28 words
The composition of claim 1 further comprising additives selected from the group comprising preservatives, whiteners, dyes, fluorides, antitartar and anticalculus agents, chlorophyll compounds, ammoniated materials, flavorings and sweeteners.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1996-01-05 | 1996-07-06 | 1997-01-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-01-05 | 2000-07-06 | 2001-01-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-01-05 | 2004-07-06 | 2005-01-05 | $7700.00 | Window Closed / Lapsed |