Compound and detectable molecules having an oligo- or polynucleotide with modifiable reactive group

Abstract

Abstract A detectable molecule of the formula A.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.m where A 3 is A 2 or a polymer, where A 3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## or a C 1 -C 10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R 2 -- where R 2 is a C 1 -C 10 branched or unbranched alkyl; Z a is chlorine, bromine or iodine; E is O, NH or an acyclic divalent sulfur atom; Det b is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR2## or the 4-hydroxy or acyloxy derivative thereof, where R 3 is C 1 -C 4 alkyl or CH 2 COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C 1 -C 10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A 3 . The detectable molecules are useful in in vitro or in vivo assays or therapy.

Inventors

• 👤 Jannis G. Stavrianopoulos

Bibliographic Details