US4904582A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Novel amphiphilic nucleic acid conjugates
Assignee: Synthetic Genetics Inc
•
Filing: 1987-06-11
•
Granted: 1990-02-27
•
Expires: 2007-06-11
Abstract
Abstract Novel oligonucleotide conjugates are provided, where oligonucleotides are joined through a linking arm to a hydrophobic moiety. The resulting conjugates are more efficient in membrane transport, so as to be capable of crossing the membrane and effectively modulating a transcriptional system. In this way, the compositions can be used in vitro and in vivo, for studying cellular processes, protecting mammalian hosts from pathogens, and the like.
Inventors
- 👤 Richard H. Tullis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:18
CPC Classifications
C; C07; C07H; C07H21/00; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/56; A61K47/59; A61K47/60; A61P; A61P31/00; A61P31/04; A61P31/12; A61P33/00; A61P33/02; A61P35/00; A61P37/00; A61P37/02; A61P37/06
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
70 words
A method for inhibiting the maturation or translation of a messenger RNA in a cell, said method comprising: contacting said cell with a composition comprising an oligonucleotide sequence complementary to a transcription product of said cell and a group covalently linked to said oligonucleotide sequence to provide an amphiphilic molecule, whereby said composition migrates into the cell interior resulting in the inhibition of maturation and/or translation of said transcription product.
Claim 2
Independent Claim
70 words
A method for inhibiting the maturation or translation of a messenger RNA in a cell, said method comprising: contacting said cell with a composition comprising an oligonucleotide sequence complementary to a transcription product of said cell and a group covalently linked to said oligonucleotide sequence to provide an amphiphilic molecule, whereby said composition migrates into the cell interior resulting in the inhibition of maturation and/or translation of said transcription product.
Claim 3
Dependent Claim
21 words
A method according to claim 1, wherein said cell is in culture and said composition is introduced into the nutrient medium.
Claim 4
Dependent Claim
17 words
A method according to claim 1, wherein said oligonucleotide is of from about 6 to 30 nucleotides.
Claim 5
Dependent Claim
20 words
A method according to claim 3, wherein at least one of said oligonucleotides has a phosphate as the phosphorus moiety.
Claim 6
Dependent Claim
31 words
A method according to claim 3, wherein at least one of said oligonucleotides has a phosphonate with an alkyl group of from 1 to 3 carbon atoms as the phosphorus moiety.
Claim 7
Dependent Claim
14 words
A method according to claim 1, wherein said group is a hydrophobic aromatic group.
Claim 8
Dependent Claim
14 words
A method according to claim 7, wherein said aromatic group is a trityl group.
Claim 9
Dependent Claim
14 words
A method according to claim 7, wherein said aromatic group is a fluorescein group.
Claim 10
Dependent Claim
24 words
A method according to claim 1, wherein said group is a polyalkyleneoxy group, wherein said alkylenes are of from 2 to 10 carbon atoms.
Claim 11
Dependent Claim
17 words
A method according to claim 9, wherein said polyalkyleneoxy group is from about 6 to 200 units.
Claim 12
Dependent Claim
34 words
A cell comprising a composition comprising an oligonucleotide sequence complementary to a transcription product of said cell and an amphiphilic or hydrophobic group covalently linked to said oligonucleotide sequence to provide an amphiphilic molecule.
Claim 13
Dependent Claim
34 words
A cell comprising a composition comprising an oligonucleotide sequence complementary to a transcription product of said cell and an amphiphilic or hydrophobic group covalently linked to said oligonucleotide sequence to provide an amphiphilic molecule.
Claim 14
Dependent Claim
12 words
A cell according to claim 11, wherein said cell is in culture.
Claim 15
Dependent Claim
57 words
A composition of matter comprising: an oligonucleotide sequence of at least six nucleotides complementary to a transcriptional product of a cell; an amphiphilic group comprising a polyalkyleneoxy group, wherein said alkylenes are of from 2 to 10 carbon atoms; a linker of at least one atom covalently bonded to said oligonucleotide sequence and to said amphiphilic group.
Claim 16
Dependent Claim
57 words
A composition of matter comprising: an oligonucleotide sequence of at least six nucleotides complementary to a transcriptional product of a cell; an amphiphilic group comprising a polyalkyleneoxy group, wherein said alkylenes are of from 2 to 10 carbon atoms; a linker of at least one atom covalently bonded to said oligonucleotide sequence and to said amphiphilic group.
Claim 17
Dependent Claim
19 words
A composition of matter according to claim 13, wherein said oligonucleotide is of from about 6 to 30 nucleotides.
Claim 18
Dependent Claim
22 words
A composition of matter according to claim 13, wherein at least one of said oligonucleotides has a phosphate as the phosphorus moiety.
Claim 19
Dependent Claim
33 words
A composition of matter according to claim 13, wherein at least one of said oligonucleotides has a phosphonate with an alkyl group of from 1 to 3 carbon atoms as the phosphorus moiety.
Claim 20
Dependent Claim
24 words
A composition of matter according to claim 13, wherein said linking group includes at least one of an amino, quinone, thioether, or amide group.
Claim 21
Dependent Claim
22 words
A composition of matter according to claim 13, wherein said oligonucleotide sequence is complementary at least in part to a non-coding region.
Claim 22
Dependent Claim
22 words
A composition of matter according to claim 13, wherein said oligonucleotide sequence is complementary at least in part to a coding region.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-02-26 | 1993-08-28 | 1994-02-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-02-26 | 1997-08-28 | 1998-02-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-02-26 | 2001-08-28 | 2002-02-27 | $7700.00 | Window Closed / Lapsed |