Patent US5164298A successfully refreshed from Google Patents.
US5164298A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Verocytotoxin receptor assay
Assignee: Hsc Research Development Corp
•
Filing: 1988-06-24
•
Granted: 1992-11-17
•
Expires: 2008-06-24
Abstract
Abstract This invention relates to the identification of verocytotoxin receptors of the formula: X--O--Y(R) (I) wherein Y is sphingosine, hydroxylated sphinogosine or saturated sphingosine, wherein X is selected from said group and optionally a polysaccharide linking X to the --O--Y(R) group, and wherein R is H, or a fatty acid and R is linked to the amine moiety of the sphingosine, in combination with an assay component and their use in novel receptor-binding assays for the detection and quantitation of verocytotoxins.
Inventors
- 👤 Clifford A. Lingwood
- 👤 Mohammed A. Karmali
- 👤 Magdy T. Basta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 02:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92; G01N33/53; G01N33/569; G01N33/56911; G01N33/56916; Y; Y10; Y10S; Y10S435/00; Y10S435/975; Y10S436/00; Y10S436/829
Claims Analysis
Total Claims: 42
Claim 1
Independent Claim
109 words
A receptor assay kit for use in a receptor assay for the detection of the presence of verocytotoxin, said receptor assay kit comprising a receptor and a component for performing the specific binding assay, said receptor being represented by the formula I: --X--O--Y (R) (I) wherein Y is sphingosine, hydroxylated sphingosine or saturated sphingosine, wherein X is a carbohydrate moiety selected from the group consisting of: Gal(α1-4)Gal, Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal, GalNac(β1-3)Gal and GalNac, and wherein i) R is H, or a fatty acid, ii) R is linked to the amine moiety of the sphingosine; and iii) X is other than Gal(α1-4)Gal or Gal(α1-4)Gal(β1-4)Glc when R is said fatty acid.
Claim 2
Dependent Claim
109 words
A receptor assay kit for use in a receptor assay for the detection of the presence of verocytotoxin, said receptor assay kit comprising a receptor and a component for performing the specific binding assay, said receptor being represented by the formula I: --X--O--Y (R) (I) wherein Y is sphingosine, hydroxylated sphingosine or saturated sphingosine, wherein X is a carbohydrate moiety selected from the group consisting of: Gal(α1-4)Gal, Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal, GalNac(β1-3)Gal and GalNac, and wherein i) R is H, or a fatty acid, ii) R is linked to the amine moiety of the sphingosine; and iii) X is other than Gal(α1-4)Gal or Gal(α1-4)Gal(β1-4)Glc when R is said fatty acid.
Claim 3
Dependent Claim
15 words
A receptor assay kit of claim 1 wherein R is H and X is Gal(α1-4)Gal(β1-4)-Glc.
Claim 4
Dependent Claim
17 words
A receptor assay kit of claim 1 wherein R is said fatty acid and X is GalNac(β1-3)Gal(α1-4)Gal(β1-4)Glc.
Claim 5
Dependent Claim
39 words
A receptor assay kit of claim 1 wherein said receptor is bound to a component for performing said assay selected from the group consisting of a microtitre plate, a test tube, glass beads, nitrocellulose, latex particles, proteins and liposomes.
Claim 6
Dependent Claim
26 words
A receptor assay kit of claim 1 wherein said component is the surface of a cell and said receptor is on the surface of said cell.
Claim 7
Dependent Claim
21 words
A receptor assay kit of claim 1, wherein said component is a liposome and said receptor is incorporated in said liposome.
Claim 8
Dependent Claim
11 words
The receptor assay kit of claim 1 wherein R is H.
Claim 9
Dependent Claim
11 words
The receptor assay kit of claim 7 wherein X is Gal(α1-4)Gal.
Claim 10
Dependent Claim
11 words
The receptor assay kit of claim 7 wherein X is GalNAc(β1-3)Gal(α1-4)Gal(β1-4)Glc.
Claim 11
Dependent Claim
13 words
The receptor assay kit of claim 1 wherein R, is a fatty acid.
Claim 12
Dependent Claim
17 words
The receptor assay kit of claim 10, wherein said fatty acid is represented by the formula: ##STR2##
Claim 13
Dependent Claim
31 words
The receptor assay kit of claim 2, 3, 7, 8, 9, 10 or 11 wherein said component is a microtitre plate and said receptor is bound to wells of said plate.
Claim 14
Dependent Claim
31 words
The receptor assay kit of claim 2, 3, 7, 8, 9, 10 or 11 wherein said receptor is incorporated in a liposome which is bound to wells of a microtitre plate.
Claim 15
Dependent Claim
113 words
A receptor assay for detecting the presence of verocytotoxin in a specimen comprising the steps of: contacting said specimen with a receptor for said verocytotoxin; and assaying for binding of said verocytotoxin to said receptor, wherein said receptor is represented by formula I: X--O--Y (R) (I) wherein Y is sphingosine, hydroxylated sphingosine or saturated sphingosine, wherein X is a carbohydrate moiety selected from the group consisting of: Gal(α1-4)Gal, Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal, GalNac(β1-3)Gal and GalNac, wherein i) R is H or a chemical group non-inhibitory to receptor binding, ii) R is linked to the amine moiety of the sphingosine, and iii) X is other than Gal(α1-4)Gal or Gal(α1-4)Gal(β1-4)Glc when R is said fatty acid.
Claim 16
Dependent Claim
113 words
A receptor assay for detecting the presence of verocytotoxin in a specimen comprising the steps of: contacting said specimen with a receptor for said verocytotoxin; and assaying for binding of said verocytotoxin to said receptor, wherein said receptor is represented by formula I: X--O--Y (R) (I) wherein Y is sphingosine, hydroxylated sphingosine or saturated sphingosine, wherein X is a carbohydrate moiety selected from the group consisting of: Gal(α1-4)Gal, Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal, GalNac(β1-3)Gal and GalNac, wherein i) R is H or a chemical group non-inhibitory to receptor binding, ii) R is linked to the amine moiety of the sphingosine, and iii) X is other than Gal(α1-4)Gal or Gal(α1-4)Gal(β1-4)Glc when R is said fatty acid.
Claim 17
Dependent Claim
13 words
The assay of claim 14 wherein said fatty acid has the formula: ##STR3##
Claim 18
Dependent Claim
29 words
The assay of claim 14 wherein the verocytotoxin is verocytotoxin 1 and wherein R is H and X is selected from the group consisting of Gal (α1-4)Gal and Gal(α1-4)Gal(β1-4)Glc.
Claim 19
Dependent Claim
11 words
The assay of claim 15 wherein the verocytotoxin is verocytotoxin 2.
Claim 20
Dependent Claim
26 words
The assay of claim 15 wherein the verocytotoxin is verocytotoxin 2 and wherein X is selected from the group consisting of GalNac(β1-3)Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal, GalNac(β1-3)Gal and GalNAc.
Claim 21
Dependent Claim
26 words
The assay of claim 14 wherein the verocytotoxin is oedema disease toxin and X is selected from the group consisting of GalNac(β1-3)Gal(α1-4)Gal-(β1-4)Glc, GalNAc(β1-3)Gal(α1-4)Gal, GalNAc(β1-3)Gal and GalNAc.
Claim 22
Dependent Claim
13 words
The assay of claim 14 wherein the verocytotoxin is shig a like toxin.
Claim 23
Dependent Claim
29 words
The assay of claim 14 wherein said assay is selected from the group of assays consisting of ELISA assays, radioimmunoassays, thin layer chromatography assays, cytotoxicity assays and agglutination assays.
Claim 24
Dependent Claim
18 words
The assay of claim 21 wherein said receptor is adsorbed or bound to microtitre plates or test tubes.
Claim 25
Dependent Claim
16 words
The assay of claim 22 wherein said microtitre plates or test tubes are constructed of plastic.
Claim 26
Dependent Claim
21 words
The assay of claim 23 wherein said plastic is selected from the group of plastics consisting of polyvinyl chloride and polystyrene.
Claim 27
Dependent Claim
20 words
The assay of claim 21 wherein said receptor is covalently bound to microtiter plates, test tubes, glass beads or proteins.
Claim 28
Dependent Claim
16 words
The assay of claim 14 wherein said assay is a fluorescence based receptor assay for verocytotoxin.
Claim 29
Dependent Claim
19 words
An assay of claim 14, 18 or 19 wherein said X is linked to --O--Y (R) by a polysaccharide.
Claim 30
Dependent Claim
17 words
An assay of claim 16 wherein X is linked to --O--Y(R) by a polymer, polysaccharide or peptide.
Claim 31
Dependent Claim
9 words
An assay of claim 14 wherein R is H.
Claim 32
Dependent Claim
9 words
An assay of claim 29 wherein X is Gal(α1-4)Gal.
Claim 33
Dependent Claim
9 words
An assay of claim 29 wherein X is Gal(α1-4)Gal(β1-4)Glc.
Claim 34
Dependent Claim
9 words
An assay of claim 29 wherein X is GalNAc(β1-3)Gal(α1-4)Gal(β1-4)Glc.
Claim 35
Dependent Claim
11 words
An assay of claim 14 wherein R, is a fatty acid.
Claim 36
Dependent Claim
22 words
A receptor assay of claim 29, 30, 31, 32 or 33, wherein said receptor is bound to wells of a microtitre plate.
Claim 37
Dependent Claim
26 words
A receptor assay of claim 29, 30, 31, 32, or 33, wherein said receptor is incorporated in a liposome bound to wells of a microtitre plate.
Claim 38
Dependent Claim
92 words
A microtitre plate for use in a receptor assay, said plate comprising at least one microtitre well having bound thereto a receptor represented by formula I: X-O-Y (R) (I) wherein Y is sphingosine, hydroxylated sphingosine or saturated sphingosine, X is a carbohydrate moiety selected from the group consisting of: Gal(α1-4)Gal, Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal, GalNac(β1-3)Gal and GalNac, and wherein i) R is H or a fatty acid; ii) R is linked to the amine moiety of the sphingosine; and iii) X is other than Gal(α1-4)Gal or Gal(α1-4)Gal(β1-4)Glc when R is said fatty acid.
Claim 39
Dependent Claim
92 words
A microtitre plate for use in a receptor assay, said plate comprising at least one microtitre well having bound thereto a receptor represented by formula I: X-O-Y (R) (I) wherein Y is sphingosine, hydroxylated sphingosine or saturated sphingosine, X is a carbohydrate moiety selected from the group consisting of: Gal(α1-4)Gal, Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal(β1-4)Glc, GalNac(β1-3)Gal(α1-4)Gal, GalNac(β1-3)Gal and GalNac, and wherein i) R is H or a fatty acid; ii) R is linked to the amine moiety of the sphingosine; and iii) X is other than Gal(α1-4)Gal or Gal(α1-4)Gal(β1-4)Glc when R is said fatty acid.
Claim 40
Dependent Claim
14 words
A microtitre plate of claim 36 wherein R is H and X is Gal(α1-4)Gal(β1-4)-Glc.
Claim 41
Dependent Claim
16 words
A microtitre plate of claim 36 wherein R is said non-fatty acid and X is GalNac(β1-3)Gal(α1-4)Gal(β1-4)Glc.
Claim 42
Dependent Claim
22 words
A microtitre plate of claim 26, 37 or 38 wherein said receptor is incorporated in a liposome bound to said microtitre well.
References Cited (Backward Citations)
US4415733A
Cited
US4411888A
Cited
US4476119A
Cited
US4469795A
Cited
US4521593A
Cited
US4723205A
Cited
US4863852A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-17 | 1996-05-18 | 1996-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-17 | 2000-05-18 | 2000-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-17 | 2004-05-18 | 2004-11-17 | $7700.00 | Window Closed / Lapsed |