US4411888A
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Composition of a novel immunogen for protection against diarrheal disease caused by enterotoxigenic Escherichia coli
Assignee: University of Rochester
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Filing: 1982-06-03
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Granted: 1983-10-25
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Expires: 2002-06-03
Abstract
Abstract PCT No. PCT/US82/00763 Sec. 371 Date Jun. 3, 1982 Sec. 102(e) Date Jun. 3, 1982 PCT Filed Jun. 3, 1982 PCT Pub. No. WO83/00018 PCT Pub. Date Jan. 6, 1983.There is disclosed a novel immunogen which can be used for immunological protection against acute diarrheal disease caused by enterotoxigenic strains of Escherichia coli. The novel immunogen is provided by cross-linking the E. coli heat-stable enterotoxin with the E. coli heat-labile enterotoxin (either the complete holotoxin or just the B subunit of this toxin) by a conjugation process in the presence of a conjugating agent, typically the water soluble carbodiimide, 1-ethyl-3-(3-diamethylaminopropyl) carbodiimide. This results in a unique new molecule in that the toxic properties of each individual toxin are greatly reduced, the heat-labile toxin (or its B subunit) retains its antigenicity, and the heat-stable toxin acquires immunogenicity as a function of the reaction. Immunization with the cross-linked immunogen provides immunological protection in mammals against water secretion induced by strains of E. coli which produce the heat-labile or heat-stable enterotoxins, either singly or together.
Inventors
- 👤 Frederick A. Klipstein
- 👤 Richard E. Engert
- 👤 John D. Clements
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 11:34
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/02; A61K39/107; A61K39/025; A61K39/0258; A61K2039/60; A61K2039/6031; A61K2039/6037; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
87 words
A composition for immunization of mammals against diarrheal disease comprising the product provided by the process which comprises reacting heat-stable (ST) enterotoxin of Escherichia coli with a reactant selected from the group consisting of heat-labile (LT) enterotoxin of Escherichia coli, the B subunit of said heat labile (LT) enterotoxin, cholera toxin (CT) holotoxin of Vibrio cholerae, and the B subunit of said cholera toxin (CT) holotoxin at a molar ratio of ST to reactant of at least 1 to 1 in the presence of a conjugating reagent.
Claim 2
Dependent Claim
87 words
A composition for immunization of mammals against diarrheal disease comprising the product provided by the process which comprises reacting heat-stable (ST) enterotoxin of Escherichia coli with a reactant selected from the group consisting of heat-labile (LT) enterotoxin of Escherichia coli, the B subunit of said heat labile (LT) enterotoxin, cholera toxin (CT) holotoxin of Vibrio cholerae, and the B subunit of said cholera toxin (CT) holotoxin at a molar ratio of ST to reactant of at least 1 to 1 in the presence of a conjugating reagent.
Claim 3
Dependent Claim
49 words
The composition of claim 1 in combination with a suitable adjuvant to provide an effective, nontoxic immunogen for use in immunization to protect against diarrheal disease caused by strains of homologous and heterologous somatic serotypes of Escherichia coli which produce the heat-labile or heat-stable enterotoxins, either singly or together.
Claim 4
Dependent Claim
23 words
The composition of claim 1 wherein the molar ratio of ST to reactant in the reaction mixture is at least 5 to 1.
Claim 5
Dependent Claim
18 words
The composition of claim 1 wherein the conjugating reagent is a water-soluble carbodiimide or other effective conjugation reagent.
Claim 6
Dependent Claim
11 words
The composition of claim 4 wherein the carbodiimide is 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.
Claim 7
Dependent Claim
22 words
The composition of claim 1 wherein the reaction is allowed to proceed for a period of time in excess of 1 hour.
Claim 8
Dependent Claim
29 words
The composition of claim 1 in combination with a suitable adjuvant to provide an effective immunogen for immunological protection against diarrheal disease due to strains of enterotoxigenic Escherichia coli.
Claim 9
Dependent Claim
19 words
The composition of claim 7 wherein the adjuvant is Freund's complete adjuvant or other suitable adjuvants such as alum.
Claim 10
Dependent Claim
13 words
The composition of claim 1 wherein the reactant is the heat-labile (LT) enterotoxin.
Claim 11
Dependent Claim
16 words
The composition of claim 1 wherein the reactant is the B subunit of heat-labile (LT) enterotoxin.
Claim 12
Dependent Claim
13 words
The composition of claim 1 wherein the reactant is cholera toxin (CT) holotoxin.
Claim 13
Dependent Claim
17 words
The composition of claim 1 wherein the reactant is the B subunit of cholera toxin (CT) holotoxin.
Claim 14
Dependent Claim
28 words
A method of immunization of mammals against diarrheal disease due to strains of enterotoxigenic Escherichia coli which comprises administering an effective amount of the composition of claim 1.
Claim 15
Dependent Claim
11 words
The method of claim 13 wherein the composition is administered parenterally.
Claim 16
Dependent Claim
11 words
The method of claim 13 wherein the composition is administered perorally.
Claim 17
Dependent Claim
17 words
The method of claim 13 wherein the reactant is the heat-labile (LT) enterotoxin or its B subunit.
Claim 18
Dependent Claim
17 words
The method of claim 13 wherein the reactant is cholera toxin (CT) holotoxin or its B subunit.
Claim 19
Dependent Claim
10 words
The method of claim 13 wherein the mammal is human.
References Cited (Backward Citations)
US4220584A
Cited
US4314993A
Cited
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