US5149657A
Expired / Lapsed
US
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Escherichia coli expression vector encoding bioadhesive precursor protein analogs comprising three to twenty repeats of the decapeptide (Ala-Lys-Pro-Ser-Tyr-Pro-Pro-Thr-Tyr-Lys)
Assignee: Genex Corp
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Filing: 1991-02-08
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Granted: 1992-09-22
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Expires: 2011-02-08
Abstract
Abstract Recombinant production of a bioadhesive precursor protein analog comprising three, five, ten, fifteen or twenty repeated decapeptides of the formula Ala-Lys-Pro-Ser-Tyr-Pro-Pro-Thr-Tyr-Lys is disclosed.
Inventors
- 👤 Kathy J. Maugh
- 👤 David M. Anderson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 01:07
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; C07; C07K; C07K14/00; C07K14/435; C09; C09J; C09J189/00; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/71; C12N15/79; C12N15/80; C12N15/81; C12N9/16; C07K2319/00; C07K2319/01; C07K2319/02
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
160 words
An E. coli recombinant expression vector comprising plasmid pGX2287 and a DNA sequence of a bioadhesive precursor protein analog wherein said DNA sequence is selected from the group consisting of: a. X N where X=GCG AAA CCA AGT TAC CCA CCG ACC TAC AAA N=3 or 5 and G, A, T, and C represent the deoxyribonucleotides containing the bases guanine, adenine, thymine and cytosine, respectively; b. X 5 YX 5 where X, G, A, T, and C are as defined above and Y=ACG CCG GCC; c. X 5 YX 5 YX 5 Y where X, Y, G, A, T, and C are as defined above; and d. X 5 YX 5 YX 5 YX 5 Y where X, Y, G, A, T, and C are as defined above; and wherein expression of said DNA sequence in said E. coli is under the control of said plasmid's O L /P R promoter, and trpB ribosome binding site and translation initiation signal.
Claim 2
Dependent Claim
160 words
An E. coli recombinant expression vector comprising plasmid pGX2287 and a DNA sequence of a bioadhesive precursor protein analog wherein said DNA sequence is selected from the group consisting of: a. X N where X=GCG AAA CCA AGT TAC CCA CCG ACC TAC AAA N=3 or 5 and G, A, T, and C represent the deoxyribonucleotides containing the bases guanine, adenine, thymine and cytosine, respectively; b. X 5 YX 5 where X, G, A, T, and C are as defined above and Y=ACG CCG GCC; c. X 5 YX 5 YX 5 Y where X, Y, G, A, T, and C are as defined above; and d. X 5 YX 5 YX 5 YX 5 Y where X, Y, G, A, T, and C are as defined above; and wherein expression of said DNA sequence in said E. coli is under the control of said plasmid's O L /P R promoter, and trpB ribosome binding site and translation initiation signal.
Claim 3
Dependent Claim
11 words
An E. coli cell comprising the recombinant vector of claim 1.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-22 | 1996-03-23 | 1996-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-22 | 2000-03-23 | 2000-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-22 | 2004-03-23 | 2004-09-22 | $7700.00 | Window Closed / Lapsed |