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US5149657A Expired / Lapsed US ⏱️ 0.0 years remaining

Escherichia coli expression vector encoding bioadhesive precursor protein analogs comprising three to twenty repeats of the decapeptide (Ala-Lys-Pro-Ser-Tyr-Pro-Pro-Thr-Tyr-Lys)

Assignee: Genex Corp
•
Filing: 1991-02-08
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Granted: 1992-09-22
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Expires: 2011-02-08
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Abstract

Abstract Recombinant production of a bioadhesive precursor protein analog comprising three, five, ten, fifteen or twenty repeated decapeptides of the formula Ala-Lys-Pro-Ser-Tyr-Pro-Pro-Thr-Tyr-Lys is disclosed.

Inventors

  • 👤 Kathy J. Maugh
  • 👤 David M. Anderson

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-25 01:07
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; C07; C07K; C07K14/00; C07K14/435; C09; C09J; C09J189/00; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/71; C12N15/79; C12N15/80; C12N15/81; C12N9/16; C07K2319/00; C07K2319/01; C07K2319/02

Claims Analysis

Total Claims: 3
Claim 1
Independent Claim 160 words
An E. coli recombinant expression vector comprising plasmid pGX2287 and a DNA sequence of a bioadhesive precursor protein analog wherein said DNA sequence is selected from the group consisting of: a. X N where X=GCG AAA CCA AGT TAC CCA CCG ACC TAC AAA N=3 or 5 and G, A, T, and C represent the deoxyribonucleotides containing the bases guanine, adenine, thymine and cytosine, respectively; b. X 5 YX 5 where X, G, A, T, and C are as defined above and Y=ACG CCG GCC; c. X 5 YX 5 YX 5 Y where X, Y, G, A, T, and C are as defined above; and d. X 5 YX 5 YX 5 YX 5 Y where X, Y, G, A, T, and C are as defined above; and wherein expression of said DNA sequence in said E. coli is under the control of said plasmid's O L /P R promoter, and trpB ribosome binding site and translation initiation signal.
Claim 2
Dependent Claim 160 words
An E. coli recombinant expression vector comprising plasmid pGX2287 and a DNA sequence of a bioadhesive precursor protein analog wherein said DNA sequence is selected from the group consisting of: a. X N where X=GCG AAA CCA AGT TAC CCA CCG ACC TAC AAA N=3 or 5 and G, A, T, and C represent the deoxyribonucleotides containing the bases guanine, adenine, thymine and cytosine, respectively; b. X 5 YX 5 where X, G, A, T, and C are as defined above and Y=ACG CCG GCC; c. X 5 YX 5 YX 5 Y where X, Y, G, A, T, and C are as defined above; and d. X 5 YX 5 YX 5 YX 5 Y where X, Y, G, A, T, and C are as defined above; and wherein expression of said DNA sequence in said E. coli is under the control of said plasmid's O L /P R promoter, and trpB ribosome binding site and translation initiation signal.
Claim 3
Dependent Claim 11 words
An E. coli cell comprising the recombinant vector of claim 1.

References Cited (Backward Citations)

EP0035384A2 Cited
US4721673A Cited
US4585585A Cited
US4687740A Cited
GB2162190A Cited
JPS6185400A Cited
US4798791A Cited
US5049504A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1995-09-22 1996-03-23 1996-09-22 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1999-09-22 2000-03-23 2000-09-22 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2003-09-22 2004-03-23 2004-09-22 $7700.00 Window Closed / Lapsed

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