US5049504A
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Bioadhesive coding sequences
Assignee: Genex Corp
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Filing: 1990-05-30
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Granted: 1991-09-17
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Expires: 2010-05-30
Abstract
Abstract Vectors are provided that are capable of expressing, in microbial transformants, a protein having the native amino acid sequence of a bioadhesive precursor protein of a marine animal selected from the group consisting of mussels, barnacles, and oysters. The bioadhesive precursor protein can be expressed in transformants, recovered and converted to a bioadhesive protein by hydroxylation.
Inventors
- 👤 Kathy J. Maugh
- 👤 David M. Anderson
- 👤 Robert Strausberg
- 👤 Susan L. Strausberg
- 👤 Russ McCandliss
- 👤 Tena Wei
- 👤 David Filpula
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:02
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; C07; C07K; C07K14/00; C07K14/435; C09; C09J; C09J189/00; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/71; C12N15/79; C12N15/80; C12N15/81; C12N9/16; C07K2319/00; C07K2319/01; C07K2319/02
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
83 words
An isolated DNA segment comprising codons which encode the native amino acid sequence of a bioadhesive precursor protein of a marine animal selected from the group consisting of mussels and barnacles, said protein being capable of function as a bioadhesive after expression and upon hydroxylation and oxidation, or an isolated DNA segment comprising codons which encode the native amino acid sequence of a fragment of said protein, said fragment being capable of function as a bioadhesive after expression and upon hydroxylation and oxidation
Claim 2
Dependent Claim
83 words
An isolated DNA segment comprising codons which encode the native amino acid sequence of a bioadhesive precursor protein of a marine animal selected from the group consisting of mussels and barnacles, said protein being capable of function as a bioadhesive after expression and upon hydroxylation and oxidation, or an isolated DNA segment comprising codons which encode the native amino acid sequence of a fragment of said protein, said fragment being capable of function as a bioadhesive after expression and upon hydroxylation and oxidation
Claim 3
Dependent Claim
15 words
A DNA segment as claimed in claim 1 wherein said marine animal is a mussel.
Claim 4
Dependent Claim
16 words
A DNA segment as claimed in claim 2 wherein said mussel is of the genus Mytilus.
Claim 5
Dependent Claim
21 words
A DNA segment as claimed in claim 3, wherein the encoded bioadhesive precursor protein is of the mussel species Mytilus edulis.
Claim 6
Dependent Claim
44 words
A DNA segment as claimed in claim 4 comprising codons for a bioadhesive precursor protein which contains the sequence: ##STR4## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 7
Dependent Claim
44 words
A DNA segment as claimed in claim 4, coding for a bioadhesive precursor protein having the amino acid sequence: ##STR5## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 8
Dependent Claim
44 words
A DNA segment as claimed in claim 4, coding for a bioadhesive precursor protein having the amino acid sequence: ##STR6## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 9
Dependent Claim
44 words
A DNA segment as claimed in claim 4, coding for a bioadhesive precursor protein having the amino acid sequence: ##STR7## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 10
Dependent Claim
44 words
A DNA segment as claimed in claim 4, coding for a bioadhesive precursor protein having the amino acid sequence: ##STR8## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 11
Dependent Claim
38 words
A DNA segment as claimed in claim 4, comprising the following DNA sequence: ##STR9## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 12
Dependent Claim
38 words
A DNA segment as claimed in claim 4, comprising the following DNA sequence: ##STR10## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 13
Dependent Claim
38 words
A DNA segment as claimed in claim 4, comprising the following DNA sequence: ##STR11## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 14
Dependent Claim
38 words
A DNA segment as claimed in claim 4 comprising the following DNA sequence: ##STR12## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 15
Dependent Claim
38 words
A DNA segment as claimed in claim 4 comprising the following DNA sequence: ##STR13## or a fragment of said sequence said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 16
Dependent Claim
119 words
A vector for expression of a bioadhesive precursor protein comprising: (a) a DNA segment comprising codons which encode the native amino acid sequence of a bioadhesive precursor protein of a marine animal selected from the group consisting of mussels and barnacles, said protein being capable of function as a bioadhesive after expression and upon hydroxylation and oxidation, or an isolated DNA segment comprising codons which encode the native amino acid sequence of a fragment of said protein, said fragment being capable of function as a bioadhesive after expression and upon hydroxylation and oxidation; and (b) a promoter and transcription initiation signals, operably linked to said DNA segment, which are capable of effecting microbial expression of said bioadheisve precursor protein.
Claim 17
Dependent Claim
119 words
A vector for expression of a bioadhesive precursor protein comprising: (a) a DNA segment comprising codons which encode the native amino acid sequence of a bioadhesive precursor protein of a marine animal selected from the group consisting of mussels and barnacles, said protein being capable of function as a bioadhesive after expression and upon hydroxylation and oxidation, or an isolated DNA segment comprising codons which encode the native amino acid sequence of a fragment of said protein, said fragment being capable of function as a bioadhesive after expression and upon hydroxylation and oxidation; and (b) a promoter and transcription initiation signals, operably linked to said DNA segment, which are capable of effecting microbial expression of said bioadheisve precursor protein.
Claim 18
Dependent Claim
16 words
A vector as claimed in claim 15 wherein the encoded bioadhesive precursor protein is from mussel.
Claim 19
Dependent Claim
20 words
A vector as claimed in claim 16, wherein the encoded bioadhesive precursor protein is of the mussel species Mytilus edulis.
Claim 20
Dependent Claim
48 words
A vector as claimed in claim 17, wherein the DNA segment (a) comprises codons for a bioadhesive precursor comprising the amino acid sequence: ##STR14## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 21
Dependent Claim
48 words
A vector as claimed in claim 17, wherein the DNA segment (a) comprises codons for a bioadhesive precursor comprising the amino acid sequence: ##STR15## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 22
Dependent Claim
49 words
A vector as claimed in claim 17, wherein the DNA segment (a) comprises codons for a bioadhesive precursor protein comprising the amino acid sequence: ##STR16## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 23
Dependent Claim
49 words
A vector as claimed in claim 17 wherein the DNA segment (a) comprises codons for a bioadhesive precursor protein comprising the amino acid sequence: ##STR17## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 24
Dependent Claim
49 words
A vector as claimed in claim 17 wherein the DNA segment (a) comprises codons for a bioadhesive precursor protein comprising the amino acid sequence: ##STR18## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 25
Dependent Claim
44 words
A vector as claimed in claim 17, wherein the DNA segment (a) consists of the following sequence of bases: ##STR19## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 26
Dependent Claim
44 words
A vector as claimed in claim 17, wherein the DNA segment (a) consists of the following sequence of bases: ##STR20## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 27
Dependent Claim
44 words
A vector as claimed in claim 17, wherein the DNA segment (a) consists of the following sequence of bases: ##STR21## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 28
Dependent Claim
43 words
A vector as claimed in claim 17, wherein the DNA segment (a) comprises the following sequence of bases: ##STR22## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 29
Dependent Claim
43 words
A vector as claimed in claim 17, wherein the DNA segment (a) comprises the following sequence of bases: ##STR23## or a fragment of said sequence, said fragment encoding a protein capable of function as a bioadhesive after expression and upon hydroxylation and oxidation.
Claim 30
Dependent Claim
35 words
A microorganism, said microorganism containing the expression vector of claim 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 25, 26 or 27, and said microorganism being capable of expressing a bioadhesive precursor protein.
Claim 31
Dependent Claim
11 words
The microorganism of claim 28, wherein said microorganism is E. coli.
Claim 32
Dependent Claim
11 words
The microorganism of claim 28, wherein said microorganism is S. cerevisiae.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-09-16 | 1995-03-18 | 1995-09-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-09-16 | 1999-03-18 | 1999-09-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-09-16 | 2003-03-18 | 2003-09-17 | $7700.00 | Window Closed / Lapsed |