US5112752A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biocatalytic oxidation using soybean and other legume peroxidases
Assignee: Wiley Organics Inc
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Filing: 1991-05-22
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Granted: 1992-05-12
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Expires: 2011-05-22
Abstract
Abstract Biocatalytic oxidative processes wherein oxidizable substrates are reacted with peroxides in the presence of a peroxidase such as soybean peroxidase or another legume peroxidase; an oxidative coupling reaction for producing phenolic resins; a method for the purification of peroxidase enzyme-containing extracts generally also are described.
Inventors
- 👤 Mark A. Johnson
- 👤 Alexander R. Pokora
- 👤 William L. Cyrus, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 18:41
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0065; C08; C08G; C08G61/00; C12P; C12P7/00; C12P7/02; C12P7/22; Y; Y10; Y10S; Y10S435/00; Y10S435/80; Y10S435/814; Y10S435/816; Y10S435/8215; Y10S435/911
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
124 words
A method for the purification of a peroxidase enzyme comprising forming a solution of a peroxidase enzyme and a protein fixative selected from the group consisting of tannic acid, tannins, monolignols, fulvic acids, lignan, humic acids, melanoidins, proanthcyanidins, stilbenes, depsides, lignin model compounds, soluble suberin, flavonoids, dihydroxyphenyl compounds, kerogen, gallic acid esters, phenolic acids, gallic acid amides, hexahydroxydiphenic glucose estes, polymeric phenols, bis (hydroxyphenyl) sulfones, bitumens, soluble lignite extracts, sulfonated phenols and naphthols and their copolymers, melamineglyoxal/glyoxylate-phenol/naphthol condensates; peat euphorbia, cassia, rose, tea, grape and saxifragea; sulfonated extracts, and bark extracts, and graft copolymers derived from said extracts and syntans; adding a non-solvent for said enzyme to said solution to cause said enzyme to precipitate, and redissolving said enzyme and the peroxidase is recovered.
Claim 2
Independent Claim
124 words
A method for the purification of a peroxidase enzyme comprising forming a solution of a peroxidase enzyme and a protein fixative selected from the group consisting of tannic acid, tannins, monolignols, fulvic acids, lignan, humic acids, melanoidins, proanthcyanidins, stilbenes, depsides, lignin model compounds, soluble suberin, flavonoids, dihydroxyphenyl compounds, kerogen, gallic acid esters, phenolic acids, gallic acid amides, hexahydroxydiphenic glucose estes, polymeric phenols, bis (hydroxyphenyl) sulfones, bitumens, soluble lignite extracts, sulfonated phenols and naphthols and their copolymers, melamineglyoxal/glyoxylate-phenol/naphthol condensates; peat euphorbia, cassia, rose, tea, grape and saxifragea; sulfonated extracts, and bark extracts, and graft copolymers derived from said extracts and syntans; adding a non-solvent for said enzyme to said solution to cause said enzyme to precipitate, and redissolving said enzyme and the peroxidase is recovered.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein said peroxidase enzyme is a soybean peroxidase.
Claim 4
Dependent Claim
12 words
The method of claim 2 wherein said protein fixative is tannic acid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-05-12 | 1995-11-11 | 1996-05-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-05-12 | 1999-11-11 | 2000-05-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-05-12 | 2003-11-11 | 2004-05-12 | $7700.00 | Window Closed / Lapsed |