US3769168A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the purification of amylases
Assignee: Nagase Viita Co Ltd
•
Filing: 1971-06-11
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Granted: 1973-10-30
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Expires: 1991-06-11
Abstract
Abstract The present procedure concerns a process for the purification of beta-amylases comprising treating a crude beta-amylase solution derived from wheat bran, soy bean or fresh sweet potato with an adsorbent such as bentonite, acidic clay, kaolinite and activated clay at a pH lower than 5.0 to adsorb the beta-amylases, washing out the adsorbent with water, and then eluting the adsorbed betaamylases with a solution with an ionic strength over 0.5 Mu and a pH exceeding 5.0.
Inventors
- 👤 K Masuda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 19:45
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N9/2411; C12N9/2425; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
18 words
A process according to claim 1 wherein said adsorption and elution are carried out at lower than 30*C.
Claim 2
Dependent Claim
40 words
A process according to claim 1 wherein said enzymes are adsorbed with an absorbent of the minimum amount that effects 100 percent adsorption of said enzymes on said adsorbent, and in that the elution is carried out following said adsorption.
Claim 3
Dependent Claim
34 words
A process according to claim 1 wherein an enzyme solution obtained by the extraction of fresh sweet potato is adsorbed with kaolinite or activated clay to obtain substantially pure enzymes at a higher yield.
Claim 4
Dependent Claim
23 words
A process according to claim 1 wherein enzymes derived from wheat bran are recovered at a higher yield using kaolinite or activated clay.
Claim 5
Dependent Claim
25 words
A process according to claim 1 wherein enzymes derived from soy bean are recovered at an especially high yield by adsorption with kaolinite and elution.
References Cited (Backward Citations)
US2717852A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-29 | 1977-04-30 | 1977-10-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-29 | 1981-04-30 | 1981-10-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-29 | 1985-04-30 | 1985-10-30 | $7700.00 | Window Closed / Lapsed |