US4910135A
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US
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Water-soluble peroxidase derivatives
Assignee: Roche Diagnostics GmbH
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Filing: 1986-11-14
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Granted: 1990-03-20
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Expires: 2006-11-14
Abstract
Abstract A water-soluble peroxidase derivative obtainable by the oxidation of peroxidase with periodic acid or with a salt thereof and binding to a water-soluble polymer selected from polysaccharides, polyethylene glycols, polyvinylpyrrolidones and polyacid anhydrides. Also provided is a process for the preparation of this water-soluble peroxidase derivative and for the use of this water-soluble peroxidase derivative for the determination of hydrogen peroxide.
Inventors
- 👤 Wilhelm Tischer
- 👤 Josef Heinle
- 👤 Michael-Harold Town
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 10:51
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N9/00; C12N9/96; C12Q; C12Q1/00; C12Q1/26; C12Q1/28
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
45 words
A water soluble peroxidase derivative comprising peroxidase oxidized with periodic acid or a salt thereof and bound through one or more C 1 -C 8 alkylene groups to a water-soluble activated polymer selected from the group consisting of polysaccharide, polyethylene glycol, polyvinylpyrrolidone and polyacid anhydride.
Claim 2
Dependent Claim
45 words
A water soluble peroxidase derivative comprising peroxidase oxidized with periodic acid or a salt thereof and bound through one or more C 1 -C 8 alkylene groups to a water-soluble activated polymer selected from the group consisting of polysaccharide, polyethylene glycol, polyvinylpyrrolidone and polyacid anhydride.
Claim 3
Dependent Claim
13 words
The derivative of claim 1, wherein the alkylene group is an ethylene radical.
Claim 4
Dependent Claim
11 words
The derivative of claim 2 wherein the polymer is a polysaccharide.
Claim 5
Dependent Claim
18 words
The derivative of claim 1 wherein oxidation is accomplished at pH 4-8 and a temperature of 0°-30° C.
Claim 6
Dependent Claim
11 words
The derivative of claim 1 wherein the polymer is a polysaccharide.
Claim 7
Dependent Claim
43 words
A process for the preparation of a water-soluble peroxidase derivative, comprising oxidizing peroxidase with periodic acid or with a salt thereof and binding the peroxidase to a water-soluble polymer selected from the group consisting of a polysaccharide, polyethylene glycol, polyvinylpyrrolidone and polyacid anhydride.
Claim 8
Dependent Claim
43 words
A process for the preparation of a water-soluble peroxidase derivative, comprising oxidizing peroxidase with periodic acid or with a salt thereof and binding the peroxidase to a water-soluble polymer selected from the group consisting of a polysaccharide, polyethylene glycol, polyvinylpyrrolidone and polyacid anhydride.
Claim 9
Dependent Claim
13 words
The process of claim 6, wherein binding takes place to an activated polymer.
Claim 10
Dependent Claim
23 words
The process of claim 6, wherein the peroxidase is oxidized at a temperature of about 0°-30° C. and a pH of about 4-8.
Claim 11
Dependent Claim
12 words
The process of claim 6, wherein the polymer used is a polysaccharide.
Claim 12
Dependent Claim
35 words
The process of claim 6, further comprising binding the oxidized peroxidase to the polymer by reacting the oxidized peroxidase with an alkylenediamine containing up to 8 carbon atoms to form a bridge to the polymer.
Claim 13
Dependent Claim
16 words
The process of claim 10, wherein the alkylenediamine is ethylenediamine and the polymer is activated polysaccharide.
Claim 14
Dependent Claim
20 words
The process of claim 6, wherein the peroxidase is oxidized at a temperature of 0-10° C. and pH of 5-6.
Claim 15
Dependent Claim
61 words
In a method of determining hydrogen peroxide using a peroxidase enzyme substrate to form a product which can be measured colorimetrically the improvement wherein the peroxidase is a water-soluble peroxidase derivative comprising peroxidase oxidized with periodic acid or a salt thereof and bound to a water-soluble polymer selected from the group consisting of a polysaccharide, polyethylene glycol, polyvinylpyrrolidone and polyacid anhydride.
Claim 16
Dependent Claim
61 words
In a method of determining hydrogen peroxide using a peroxidase enzyme substrate to form a product which can be measured colorimetrically the improvement wherein the peroxidase is a water-soluble peroxidase derivative comprising peroxidase oxidized with periodic acid or a salt thereof and bound to a water-soluble polymer selected from the group consisting of a polysaccharide, polyethylene glycol, polyvinylpyrrolidone and polyacid anhydride.
Claim 17
Dependent Claim
47 words
A water soluble peroxidase derivative comprising peroxidase oxidized with periodic acid or a salt thereof and bound to a water-soluble activated polymer selected from the group consisting of polysaccharide, polyethylene glycol, polyvinylpyrrolidone and polyacid anhydride wherein the polymer is bound to the peroxidase through an ethylene group.
Claim 18
Dependent Claim
47 words
A water soluble peroxidase derivative comprising peroxidase oxidized with periodic acid or a salt thereof and bound to a water-soluble activated polymer selected from the group consisting of polysaccharide, polyethylene glycol, polyvinylpyrrolidone and polyacid anhydride wherein the polymer is bound to the peroxidase through an ethylene group.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-03-19 | 1993-09-18 | 1994-03-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-03-19 | 1997-09-18 | 1998-03-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-03-19 | 2001-09-18 | 2002-03-20 | $7700.00 | Window Closed / Lapsed |