US4634671A
Expired / Lapsed
US
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Water-soluble cross-linked polymer of lysyl endopeptidase, process for preparing same and use of same
Assignee: Fujifilm Wako Pure Chemical Corp
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Filing: 1983-09-19
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Granted: 1987-01-06
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Expires: 2003-09-19
Abstract
Abstract A water-soluble cross-linked polymer of the enzyme lysyl endopeptidase produced by Achromobacter lyticus and a process for preparing the polymer as well as a semi-synthesis of human insulin using the polymer.
Inventors
- 👤 Yoshitsugu Sakata
- 👤 Akinori Shintani
- 👤 Tetsuya Matsuo
- 👤 Haruhiko Sugiyama
- 👤 Nobuyuki Tokioka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 21:42
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
69 words
A water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 prepared by the process comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 2
Dependent Claim
69 words
A water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 prepared by the process comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 3
Dependent Claim
29 words
The water-soluble polymer as claimed in claim 1, wherein polymerization is further carried out in the presence of from about 0.01 to about 10 wt% of a water-soluble protein.
Claim 4
Dependent Claim
29 words
The water-soluble polymer as claimed in claim 1, wherein polymerization is further carried out in the presence of from about 0.005 to about 20 wt% of a polyamine spacer.
Claim 5
Dependent Claim
41 words
The water-soluble polymer as claimed in claim 1, wherein polymerization is further carried out in the presence of from about 0.1 to about 10 wt% of a water-soluble protein and from about 0.005 to about 20 wt% of a polyamine spacer.
Claim 6
Dependent Claim
28 words
The water-soluble polymer as claimed in claim 2, wherein said polyaldehyde is glutaraldehyde and said polyisocyanate is selected from the group consisting of hexamethylene diisocyanate and toluene diisocyanate.
Claim 7
Dependent Claim
21 words
The water-soluble polymer as claimed in claim 3, wherein said polyamine is selected from the group consisting of spermine and spermidine.
Claim 8
Dependent Claim
21 words
The water-soluble polymer as claimed in claim 4, wherein said polyamine is selected from the group consisting of spermine and spermidine.
Claim 9
Dependent Claim
20 words
The water-soluble polymer as claimed in claim 1, wherein said Achromobacter lyticus has the identifying characteristics of ATCC No. 21456.
Claim 10
Dependent Claim
69 words
A process for preparing a water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 11
Dependent Claim
69 words
A process for preparing a water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 12
Dependent Claim
32 words
The process for preparing a water-soluble polymer as claimed in claim 9, wherein said polyaldehyde is glutaraldehyde and said polyisocyanate is selected from the group consisting of hexamethylene diisocyanate and toluene diisocyanate.
Claim 13
Dependent Claim
24 words
The process for preparing a water-soluble polymer as claimed in claim 9, wherein said Achromobacter lyticus has the identifying characteristics of ATCC No. 21456.
Claim 14
Dependent Claim
80 words
A process for preparing a water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent and about 0.01 to about 10 wt% of a water-soluble protein, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 15
Dependent Claim
80 words
A process for preparing a water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent and about 0.01 to about 10 wt% of a water-soluble protein, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 16
Dependent Claim
32 words
The process for preparing a water-soluble polymer as claimed in claim 12, wherein said polyaldehyde is glutaraldehyde and said polyisocyanate is selected from the group consisting of hexamethylene diisocyanate and toluene diisocyanate.
Claim 17
Dependent Claim
24 words
The process for preparing a water-soluble polymer as claimed in claim 12, wherein said Achromobacter lyticus has the identifying characteristics of ATCC No. 21456.
Claim 18
Dependent Claim
80 words
A process for preparing a water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent and about 0.005 to about 20 wt% of a polyamine spacer, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 19
Dependent Claim
80 words
A process for preparing a water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent and about 0.005 to about 20 wt% of a polyamine spacer, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 20
Dependent Claim
32 words
The process for preparing a water-soluble polymer as claimed in claim 15, wherein said polyaldehyde is glutaraldehyde and said polyisocyanate is selected from the group consisting of hexamethylene diisocyanate and toluene diisocyanate.
Claim 21
Dependent Claim
25 words
The process for preparing a water-soluble polymer as claimed in claim 15, wherein said polyamine is selected from the group consisting of spermine and spermidine.
Claim 22
Dependent Claim
24 words
The process for preparing a water-soluble polymer as claimed in claim 15, wherein said Achromobacter lyticus has the identifying characteristics of ATCC No. 21456.
Claim 23
Dependent Claim
90 words
A process for preparing a water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent, about 0.01 to about 10 wt% of a water-soluble protein and about 0.005 to about 20 wt% of a polyamine spacer, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 24
Dependent Claim
90 words
A process for preparing a water-soluble, intermolecularly cross-linked polymer of Achromobacter protease I having a molecular weight of between 400,000 to 700,000 comprising polymerizing, in solution, about 0.2 to about 20 wt% of Achromobacter protease I derived from Achromobacter lyticus with about 1 to about 5 wt% of a polyaldehyde or a polyisocyanate cross-linking agent, about 0.01 to about 10 wt% of a water-soluble protein and about 0.005 to about 20 wt% of a polyamine spacer, at about pH 7.0 at about 30° C. for several minutes to 30 hours.
Claim 25
Dependent Claim
32 words
The process for preparing a water-soluble polymer as claimed in claim 19, wherein said polyaldehyde is glutaraldehyde and said polyisocyanate is selected from the group consisting of hexamethylene diisocyanate and toluene diisocyanate.
Claim 26
Dependent Claim
25 words
The process for preparing a water-soluble polymer as claimed in claim 19, wherein said polyamine is selected from the group consisting of spermine and spermidine.
Claim 27
Dependent Claim
24 words
The process for preparing a water-soluble polymer as claimed in claim 19, wherein said Achromobacter lyticus has the identifying characteristics of ATCC No. 21456.
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| 3.5 Years (Year 4) | 1990-01-05 | 1990-07-07 | 1991-01-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-01-05 | 1994-07-07 | 1995-01-06 | $3760.00 | Window Closed / Lapsed |
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