US3860484A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme stabilization
Assignee: Xerox Corp
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Filing: 1972-09-28
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Granted: 1975-01-14
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Expires: 1992-09-28
Abstract
Abstract The bio-catalytic activity of oxidoreductase enzymes is stabilized by contacting the enzymes in solution with stabilizing synthetic polymers selected from polyvinyl pyrrolidone, polyethylene oxide, copolymers of polyvinyl pyrrolidone and polyvinyl acetate, and copolymers of polyvinyl alcohol and polyvinyl acetate to form an aqueous composition containing the enzyme and polymer. The aqueous composition can be converted to a dry form by freeze drying.
Inventors
- 👤 James Joseph O'malley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 04:55
CPC Classifications
A; A61; A61K; A61K47/00; A61K47/30; A61K47/32; A61K47/06; A61K47/08; A61K47/10
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
44 words
A method of stabilizing the bio-catalytic activity of heat sensitive oxidoreductase enzymes comprising freeze drying the enzyme in the presence of a polymer selected from the group consisting of copolymers of polyvinyl pyrrolidone and polyvinyl acetate, and copolymers of polyvinyl alcohol and polyvinyl acetate.
Claim 2
Independent Claim
44 words
A method of stabilizing the bio-catalytic activity of heat sensitive oxidoreductase enzymes comprising freeze drying the enzyme in the presence of a polymer selected from the group consisting of copolymers of polyvinyl pyrrolidone and polyvinyl acetate, and copolymers of polyvinyl alcohol and polyvinyl acetate.
Claim 3
Dependent Claim
27 words
The method according to claim 1 wherein the weight ratio of polymer to enzyme is from about 5 to 1 to about 5 X 104 to 1.
Claim 4
Dependent Claim
21 words
The method according to claim 1 wherein the weight ratio of polymer to enzyme is about 5 X 103 to 1.
Claim 5
Dependent Claim
12 words
The method according to claim 1 where the enzyme is glucose oxidase.
Claim 6
Dependent Claim
11 words
The composition of claim 5 wherein the enzyme is glucose oxidase.
Claim 7
Dependent Claim
26 words
The composition of claim 5 wherein the weight ratio of polymer to enzyme is from about 5 to 1 to about 5 X 104 to 1.
Claim 8
Dependent Claim
20 words
The composition of claim 5 wherein the weight ratio of polymer to enzyme is about 5 X 103 to 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-01-13 | 1978-07-15 | 1979-01-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-01-13 | 1982-07-15 | 1983-01-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-01-13 | 1986-07-15 | 1987-01-14 | $7700.00 | Window Closed / Lapsed |