US4460683A
Expired / Lapsed
US
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Soluble liver uricase with a process for the preparation thereof and with the use thereof
Assignee: Roche Diagnostics GmbH
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Filing: 1982-07-02
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Granted: 1984-07-17
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Expires: 2002-07-02
Abstract
Abstract The present invention provides liver uricase, wherein it is covalently bond to a water-soluble polysaccharide, polyacid anhydride, polyvinylpyrrolidone or acid anhydride as carrier substance and is still soluble at pH values below 6. The present invention also provides a process for the preparation of this liver uricase.
Inventors
- 👤 Manfred Gloger
- 👤 Josef Heinle
- 👤 Helmut Schlumberger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 02:17
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/08; C12N11/082; C12Q; C12Q1/00; C12Q1/62
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
29 words
Liver uricase, wherein it is covalently bound to a water-soluble polysaccharide, polyacid anhydride, polyvinylpyrrolidone or acid anhydride as carrier substance and is still soluble at pH values below 6.
Claim 2
Dependent Claim
29 words
Liver uricase, wherein it is covalently bound to a water-soluble polysaccharide, polyacid anhydride, polyvinylpyrrolidone or acid anhydride as carrier substance and is still soluble at pH values below 6.
Claim 3
Dependent Claim
19 words
Liver uricase according to claim 1, wherein it is soluble at a pH value of from 1 to 11.
Claim 4
Dependent Claim
15 words
Liver uricase according to claim 1 or 2, wherein the soluble polysaccharide is a dextran.
Claim 5
Dependent Claim
21 words
Liver uricase according to claim 1 or 2, wherein the soluble polysaccharide is soluble starch, sugar or a synthetic sugar polymer.
Claim 6
Dependent Claim
69 words
A process for the preparation of a liver uricase covalently bound to a water-soluble polysaccharide, polyacid anhydride, polyvinylpyrrolidone or acid anhydride as a carrier material, and soluble at pH values below 6, comprising the steps of reacting liver uricase in aqueous solution at a pH value of from 9 to 11 with the dissolved carrier material, which contains at least one group capable of reaction with NH 2 groups.
Claim 7
Dependent Claim
69 words
A process for the preparation of a liver uricase covalently bound to a water-soluble polysaccharide, polyacid anhydride, polyvinylpyrrolidone or acid anhydride as a carrier material, and soluble at pH values below 6, comprising the steps of reacting liver uricase in aqueous solution at a pH value of from 9 to 11 with the dissolved carrier material, which contains at least one group capable of reaction with NH 2 groups.
Claim 8
Dependent Claim
26 words
The process of claim 5, further comprising mixing the solution of carrier-bound uricase obtained from the reaction, with saccharose and/or mannitol, and thereafter lyophilizing the mixture.
Claim 9
Dependent Claim
36 words
In the process for the determination of uric acid using liver uricase to form H 2 O 2 in the measuring reaction, the improvement, wherein use is made of a liver uricase according to claim 1.
Claim 10
Dependent Claim
20 words
A process for the determination of uric acid, wherein use is made of a liver uricase according to claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-17 | 1988-01-16 | 1988-07-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-17 | 1992-01-16 | 1992-07-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-17 | 1996-01-16 | 1996-07-17 | $7700.00 | Window Closed / Lapsed |