US4273874A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Acidic uricase and process for production thereof
Assignee: KH Neochem Co Ltd
•
Filing: 1979-12-14
•
Granted: 1981-06-16
•
Expires: 1999-12-14
Abstract
Abstract Acidic uricase is produced by fermentation of a microorganism of the genus Streptomyces. The enzyme is useful for the quantitative determination of uric acid in a sample.
Inventors
- 👤 Toru Nakanishi
- 👤 Yoshimi Shigemasa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 10:48
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0012; C12N9/0044; C12N9/0046; C12Q; C12Q1/00; C12Q1/62; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
24 words
Acidic uricase having an optimum pH in the range of 4.7 to 5.1, and characterized by catalyzing the oxidation of uric acid to allantoin.
Claim 2
Dependent Claim
24 words
Acidic uricase having an optimum pH in the range of 4.7 to 5.1, and characterized by catalyzing the oxidation of uric acid to allantoin.
Claim 3
Dependent Claim
65 words
A process for producing acidic uricase which comprises culturing a microorganism belonging to the genus Streptomyces which is capable of producing acidic uricase having an optimum pH in the range of 4.7 to 5.1, and characterized by catalyzing the oxidation of uric acid to allantoin in a nutrient medium until enzymatic activity is detected in the culture liquor and thereafter recovering said acidic uricase therefrom.
Claim 4
Dependent Claim
65 words
A process for producing acidic uricase which comprises culturing a microorganism belonging to the genus Streptomyces which is capable of producing acidic uricase having an optimum pH in the range of 4.7 to 5.1, and characterized by catalyzing the oxidation of uric acid to allantoin in a nutrient medium until enzymatic activity is detected in the culture liquor and thereafter recovering said acidic uricase therefrom.
Claim 5
Dependent Claim
15 words
A process according to claim 2 wherein said microorganism belongs to the species Streptomyces gannmycicus.
Claim 6
Dependent Claim
18 words
A process according to claim 3 wherein said microorganism has the identifying characteristics of Streptomyces gannmycicus ATCC 27434.
Claim 7
Dependent Claim
26 words
A process according to claim 2 wherein said culturing is carried out at 25° to 35° C. for 1 to 3 days at about neutral pH.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-15 | 1984-12-15 | 1985-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-15 | 1988-12-15 | 1989-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-15 | 1992-12-15 | 1993-06-16 | $7700.00 | Window Closed / Lapsed |