US4062731A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of uricase from micrococcus luteus
Assignee: Genzyme Corp
•
Filing: 1976-07-21
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Granted: 1977-12-13
•
Expires: 1996-07-21
Abstract
Abstract A stable uricase preparation of high activity is produced by growing Micrococcus luteus NRRL B-8166 in a nutrient medium. Uricase preparations having activities of up to about 1000 U/liter are produced.
Inventors
- 👤 Roy Eugene Snoke
- 👤 Hugh Arthur Risley
- 👤 Charles Thomas Goodhue
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 20:35
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0012; C12N9/0044; C12N9/0046; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/859
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
26 words
A process for producing uricase which comprises growing Micrococcus luteus NRRL B-8166 in a medium to produce to uricase, and separating the uricase from the medium.
Claim 2
Dependent Claim
26 words
A process for producing uricase which comprises growing Micrococcus luteus NRRL B-8166 in a medium to produce to uricase, and separating the uricase from the medium.
Claim 3
Dependent Claim
20 words
The process for producing uricase as defined in claim 1 wherein the separating step includes ammonium sulfate precipitation and chromatography.
Claim 4
Dependent Claim
78 words
A uricase preparation comprising an uricase having the following properties: a molecular weight of about 97,000 daltons; maximum activity at a pH of 8.6, in a potassium phosphate buffer; a Michaelis constant of 3.7 × 10 -5 ; an inhibition by Fe +3 , Cn +2 , Co +2 , or Mn +2 ; a biphasic inhibitory response rate curve to Co +2 or Mn +2 ; and activation by PO 4 -3 or SO 4 -2 .
Claim 5
Dependent Claim
78 words
A uricase preparation comprising an uricase having the following properties: a molecular weight of about 97,000 daltons; maximum activity at a pH of 8.6, in a potassium phosphate buffer; a Michaelis constant of 3.7 × 10 -5 ; an inhibition by Fe +3 , Cn +2 , Co +2 , or Mn +2 ; a biphasic inhibitory response rate curve to Co +2 or Mn +2 ; and activation by PO 4 -3 or SO 4 -2 .
Claim 6
Dependent Claim
20 words
The uricase preparation of claim 3 further having a specific activity of at least 10.6 U. per mg. of protein.
Claim 7
Dependent Claim
14 words
The uricase preparation of claim 3 which is lyophilized to form a dry powder.
Claim 8
Dependent Claim
79 words
An uricase preparation comprising a uricase a molecular weight of about 97,000 daltons; a maximum activity at a pH of 8.6, in a potassium phosphate buffer; a Michaelis constant of 3.7 × 10 -5 ; an inhibition by Fe +3 , Cn +2 , or Mn +2 ; a biphasic inhibitory response rate curve to Co +2 or Mn +2 ; and activiation by PO 4 -3 or SO 4 +2 and obtained by growing Micrococcus luteus NRRL B-8166.
Claim 9
Dependent Claim
79 words
An uricase preparation comprising a uricase a molecular weight of about 97,000 daltons; a maximum activity at a pH of 8.6, in a potassium phosphate buffer; a Michaelis constant of 3.7 × 10 -5 ; an inhibition by Fe +3 , Cn +2 , or Mn +2 ; a biphasic inhibitory response rate curve to Co +2 or Mn +2 ; and activiation by PO 4 -3 or SO 4 +2 and obtained by growing Micrococcus luteus NRRL B-8166.
Claim 10
Dependent Claim
20 words
The uricase preparation of claim 6 further having a specific activity of at least 10.6 U. per mg. of protein.
Claim 11
Dependent Claim
14 words
The uricase preparation of claim 6 which is lyophilized to form a dry powder.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-12-12 | 1981-06-13 | 1981-12-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-12-12 | 1985-06-13 | 1985-12-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-12-12 | 1989-06-13 | 1989-12-13 | $7700.00 | Window Closed / Lapsed |