US3620923A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Urate oxidase and process for the production thereof
Assignee: APPLIC BOICHIMIQUES JOUJ EN JO
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Filing: 1968-03-25
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Granted: 1971-11-16
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Expires: 1988-03-25
Abstract
Abstract The invention relates to a new enzyme or urate oxidase which is very active in promoting oxidation of uric acid to allantoin. The urate oxidase is produced by fermentation under aerobic conditions of nutrient media containing sources of assimilable carbon, assimilable nitrogen and uric acid which have been seeded with suitable cultures of bacteria, fungi or yeasts. Isolation and purification of the urate oxidase is described.
Inventors
- 👤 Pierre Laboureur
- 👤 Marcel D P Brunaud
- 👤 Claude Langlois
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 21:13
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0012; C12N9/0044; C12N9/0046; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S435/8215; Y10S435/822; Y10S435/886; Y10S435/911; Y10S435/912; Y10S435/913; Y10S435/915; Y10S435/925; Y10S435/931; Y10S435/933; Y10S435/939
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
32 words
The process claimed in claim 1 in which the species is of a genus selected from the group consisting of Mucor, Rhizopus, Absidia, Fusarium, Alternaria, Penicillium, Aspergillus, Cephalosporium, Stemphylium, Macrosporium and Geotrichum.
Claim 2
Dependent Claim
32 words
The process claimed in claim 1 in which the resulting aqueous extract is treated with a water-soluble salt of a metal selected from calcium and lead in order to precipitate impurities therewith.
Claim 3
Dependent Claim
35 words
The process claimed in claim 3 in which the urate oxidase is precipitated from the resulting aqueous solution by the addition thereto of an organic liquid which is miscible with water and the precipitate separated.
Claim 4
Dependent Claim
30 words
The process claimed in claim 3 in which the urate oxidase is precipitated from the resulting aqueous solution by the addition thereto of a concentrated solution of a water-soluble salt.
Claim 5
Dependent Claim
17 words
The process claimed in claim 4 in which the precipitated urate oxidase is further purified by chromatography.
Claim 6
Dependent Claim
26 words
The process claimed in claim 3 in which the precipitated urate oxidase is passed through a column of a cellulosic ion exchange material for further purification.
Claim 7
Dependent Claim
27 words
The process claimed in claim 3 in which the urate oxidase is passed through a column of material selected from dextran gel and polyacrylamide for further purification.
Claim 8
Dependent Claim
24 words
The process claimed in claim 4 in which the precipitated urate oxidase is further purified by reprecipitation from a solution in an aqueous medium.
Claim 9
Dependent Claim
37 words
The process claimed in claim 4 in which the precipitated urate oxidase is further purified by absorption upon a substrate selected from hydroxyapatite, bentonite and alumina, subsequent extraction of the absorbate and elution with a saline solution.
Claim 11
Dependent Claim
17 words
The process claimed in claim 5 in which the precipitated urate oxidase is further purified by chromatography.
Claim 12
Dependent Claim
24 words
The process claimed in claim 5 in which the precipitated urate oxidase is further purified by reprecipitation from a solution in an aqueous medium.
Claim 13
Dependent Claim
37 words
The process claimed in claim 5 in which the precipitated urate oxidase is further purified by absorption upon a substrate selected from hydroxyapatite, bentonite and alumina, subsequent extraction of the absorbate and elution with a saline solution.
References Cited (Backward Citations)
US3475276A
Cited
US3431176A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-11-15 | 1975-05-17 | 1975-11-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-11-15 | 1979-05-17 | 1979-11-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-11-15 | 1983-05-17 | 1983-11-16 | $7700.00 | Window Closed / Lapsed |