US3767533A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing uricase
Assignee: Noda Institute for Scientific Research
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Filing: 1971-12-23
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Granted: 1973-10-23
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Expires: 1991-12-23
Abstract
Abstract Uricase is produced by incubating Corynebacterium uratoxidans nov.sp. U-23 (ATCC No. 21749), U-8 (ATCC No. 21750), U-30 (ATCC No. 21751) or U-125 (ATCC No. 21752) in a medium containing 0.01 to 1.0% (W/V) of uric acid and preferably 0.1 to 3.0% (V/V) of at least one of alcohols having 1 to 8 carbons at a pH of 5.5 to 9.0 and a temperature of 20* to 40*C under aerobic conditions, thereby producing and accumulating the extracellular uricase in the surrounding medium and recovering the same from the cultured broth. The uricase is useful as a reagent for biochemical diagnosis.
Inventors
- 👤 Z Sugisaki
- 👤 N Watanabe
- 👤 H Koyama
- 👤 T Kobayashi
- 👤 N Iguchi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 00:47
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0012; C12N9/0044; C12N9/0046; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/843
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
34 words
A process according to claim 1, wherein the microorganism is a strain, Corynebacterium uratoxidans U-23 (FERM-P No. 789, ATCC No. 21749), U-8 (ATCC No. 21750), U-30 (ATCC No. 21751) or U-125 (ATCC No. 21752).
Claim 2
Dependent Claim
19 words
A process according to claim 1, wherein the medium contains at least one alcohol having one to eight carbons.
Claim 3
Dependent Claim
19 words
A process according to claim 3, wherein the alcohol having three to six carbons is contained in the medium.
Claim 4
Dependent Claim
20 words
A process according to claim 3, wherein 0.1 to 3.0 % (V/V) of the alcohol is contained in the medium.
Claim 5
Dependent Claim
20 words
A process according to claim 1, wherein 0.01 to 1.0 % (W/V) of uric acid is contained in the medium.
Claim 6
Dependent Claim
26 words
A process according to claim 1, wherein the incubation is carried out at a pH of 5.5 to 9.0 and a temperature of 20* to 40*C.
References Cited (Backward Citations)
US3002889A
Cited
US3620923A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-22 | 1977-04-23 | 1977-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-22 | 1981-04-23 | 1981-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-22 | 1985-04-23 | 1985-10-23 | $7700.00 | Window Closed / Lapsed |