US5096831A
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Reagents for determining cations
Assignee: Bayer Corp
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Filing: 1991-05-13
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Granted: 1992-03-17
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Expires: 2011-05-13
Abstract
Abstract The invention is reagents and procedures for determining an amount of cations present in a sample, the reagent including one or more chromogenic cryptand compounds of formula I ##STR1## wherein k and j, either same or different, are equal to 1 to about 5; m and n, either same or different, are equal to 0 to about 4; a and e, either same or different, are equal to 0 to about 2; b and d, either same or different, are equal to 0 to about 5; R, either same or different, is hydrogen, lower alkyl, lower alkylidene, lower alkenyl, allyl, or aryl; and ##STR2## wherein X is CH, N or COH; and Y includes ##STR3## except that when Q is ##STR4## wherein Y is p-nitrophenylazo, 3-phenylisothiazolyl-5-azo, isothiazolyl-5-azo, thiazolyl-5-azo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino or bis-(p-dimethylaminophenyl)hydroxymethyl, then the following condition cannot be present: that simultaneously b is equal to 0 or 1, d is equal to 0 or 1, j is equal to 1, n is equal to 1 or 2, a is equal to 1, e is equal to 1, k is equal to 1 and m is equal to 2.
Inventors
- 👤 Eddy Chapoteau
- 👤 Bronislaw P. Czech
- 👤 Carl R. Gebauer
- 👤 Koon-Wah Leong
- 👤 Anand Kumar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 20:19
CPC Classifications
C; C07; C07D; C07D498/00; C07D498/02; C07D498/08; C07D498/12; C07D498/18; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; G01N33/84
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
197 words
A reagent for determining an amount of cations present in a sample, the reagent comprising: a 3.2.2 or 3.3.2 chromogenic cryptand compound of the formula I ##STR14## wherein k and j, either same or different, are equal to 1 to about 5; m and n, either same or different, are equal to 0 to about 4; a and e, either same or different, are equal to 0 to about 2; b and d, either same or different, are equal to 0 to about 5; R, either same or different, is hydrogen, lower alkyl, lower alkylidene, lower alkenyl, allyl, or aryl; and Q is ##STR15## wherein X is CH, N, or COH; and Y includes ##STR16## except that when Q is ##STR17## wherein Y is selected from the group consisting of ρ-nitrophenylazo, 3-phenyl-isothiazolyl-5-azo, isothiazolyl-5-azo, thiazolyl-5-azo, 2,4,6-trinitrophenylazo, ρ-nitro styryl, ρ-benzoquinonemonoimino and bis-(ρ-dimethylaminophenyl) hydroxymethyl, then the following conditions cannot be present: that simultaneously b is equal to 0 or 1, d is equal to 0 or 1, j is equal to 1, n is equal to 1 or 2, a is equal to 1, e is equal to 1, k is equal to 1, and m is equal to 2.
Claim 2
Dependent Claim
197 words
A reagent for determining an amount of cations present in a sample, the reagent comprising: a 3.2.2 or 3.3.2 chromogenic cryptand compound of the formula I ##STR14## wherein k and j, either same or different, are equal to 1 to about 5; m and n, either same or different, are equal to 0 to about 4; a and e, either same or different, are equal to 0 to about 2; b and d, either same or different, are equal to 0 to about 5; R, either same or different, is hydrogen, lower alkyl, lower alkylidene, lower alkenyl, allyl, or aryl; and Q is ##STR15## wherein X is CH, N, or COH; and Y includes ##STR16## except that when Q is ##STR17## wherein Y is selected from the group consisting of ρ-nitrophenylazo, 3-phenyl-isothiazolyl-5-azo, isothiazolyl-5-azo, thiazolyl-5-azo, 2,4,6-trinitrophenylazo, ρ-nitro styryl, ρ-benzoquinonemonoimino and bis-(ρ-dimethylaminophenyl) hydroxymethyl, then the following conditions cannot be present: that simultaneously b is equal to 0 or 1, d is equal to 0 or 1, j is equal to 1, n is equal to 1 or 2, a is equal to 1, e is equal to 1, k is equal to 1, and m is equal to 2.
Claim 3
Dependent Claim
27 words
The reagent of claim 1, further comprising water and at least one buffer for maintaining the pH of the reagent at between about 8 and about 12.
Claim 4
Dependent Claim
38 words
The reagent of claim 2, wherein at least one buffer is an organic buffer selected from the group consisting of cyclohexylaminopropanesulfonic acid (CHAPS), cyclohexylaminoethanesulfonic acid (CHES), triethanolamine, diethanolamine, ethanolamine, 2-napthalene sulfonic acid, salicylic acid, and ρ-toluene sulfonic acid.
Claim 5
Dependent Claim
12 words
The reagent of claim 2, further comprising a water miscible organic solvent.
Claim 6
Dependent Claim
37 words
The reagent of claim 4, wherein the solvent is selected from the group consisting of cyclic ethers, glycol ethers, amides, aliphatic alcohols, sulfoxides, and mixtures thereof; and wherein the solvent has from three to eight carbon atoms.
Claim 7
Dependent Claim
24 words
The reagent of claim 4, further comprising a surfactant, and wherein the water to organic solvent ratio is between about 1:0.5 and about 1:2.
Claim 8
Dependent Claim
32 words
The reagent of claim 1, further comprising a first organic solvent having low vapor pressure and high boiling point, and a second organic solvent that is more volatile than the first solvent.
Claim 9
Dependent Claim
42 words
The reagent of claim 7, wherein the first organic solvent is selected from the group consisting of trialkylphosphate, triarylphosphate, dialkyladipate, dialkylsebacate, and dialkylphthalate, and wherein the second organic solvent is selected from the group consisting of cyclohexanone, tetrahydrofuran, dioxane, methanol, and diethylether.
Claim 10
Dependent Claim
21 words
The reagent of claim 7, further comprising a buffer suitable for maintaining reagent pH at between about 8 and about 12.
Claim 11
Dependent Claim
24 words
The reagent of claim 1, wherein the cryptand has selectivity for sodium cations in undiluted blood samples having a high concentration of sodium cations.
Claim 12
Dependent Claim
24 words
The reagent of claim 1, wherein the cryptand has selectivity for potassium cations in undiluted blood samples having a high concentration of sodium cations.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-03-17 | 1995-09-16 | 1996-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-03-17 | 1999-09-16 | 2000-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-03-17 | 2003-09-16 | 2004-03-17 | $7700.00 | Window Closed / Lapsed |