EP0125555A2
Expired / Lapsed
EP
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Unified test means for ion determination
Assignee: Bayer Corp
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Filing: 1984-04-30
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Granted: —
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Expires: 2004-04-30
Abstract
Abstract A test means, test device and method for use for determining the presence of an ion in a test sample are dislosed. The test means comprises a substantially nonpolar, nonporous carrier matrix incorporated with an ionophore capable of forming a complex with the specific ion, and a reporter substance capable of interacting with the complex of ionophore and ion to produce a detectable response. The test device comprises an elongated support member having a upper, substantially flat face to which is affixed the test means. The test means and test device are used by contacting either with the test sample, and observing a detectable response.
Inventors
- 👤 Steven C. Charlton
- 👤 Roger L. Fleming
- 👤 Arthur L. Y. Lau
- 👤 Paul Hemmes
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 02:07
CPC Classifications
C; C07; C07D; C07D498/00; C07D498/02; C07D498/08; C07C; C07C37/00; C07C37/50; C07C45/00; C07C45/45; C07C45/46; C07C45/61; C07C45/67; C07C45/68; C07C51/00; C07C51/347; C07C57/00; C07C57/30; C07C59/00; C07C59/40; C07C59/76; C07C59/84; C07D323/00; G; G01; G01N; G01N31/00; G01N31/22; G01N31/221; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; G01N33/84; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
56 words
A test means for determining the presence of an ion in a test sample, the test means comprising a substantially nonpolar, nonporous carrier matrix incorporated with an ionophore capable of forming a complex with the specific ion, and a reporter substance capable of interacting with the complex of ionophore and ion to produce a detectable response.
Claim 2
Dependent Claim
56 words
A test means for determining the presence of an ion in a test sample, the test means comprising a substantially nonpolar, nonporous carrier matrix incorporated with an ionophore capable of forming a complex with the specific ion, and a reporter substance capable of interacting with the complex of ionophore and ion to produce a detectable response.
Claim 3
Dependent Claim
42 words
A test means according to Claim 1, characterized in that the ionophore is a coronand, a cryp - tand or a podand and preferably is valinomycin, 4,7,13,16,21-pentaoxa-l, 10-diazabicyclo [8,8,5]tri- cosane, 4,7,13,16,21,24-hexaoxa-l,10-diazabicyclo /8,8,87hexacosane, 4,7,13,18-tetraoxa-1,10-diazabicyclo /8,5,57 eicosane, 12-crown-4, 15-crown-5, 18-crown-6, dibenzo-18-crown-6, or dicyclohexane-18- crown-6.
Claim 4
Dependent Claim
60 words
A test means according to Claim 1 or 2, characterized in that the reporter substance is a compound having the structure in which X is a halogen or pseudohalogen; R is a 2-, 3-, 5-, and/or 6-position substituent selected from lower or intermediate alkyl, aryl and a fused ring at the 2,3- or 5,6-positions; and n is 0 to 4.
Claim 5
Dependent Claim
44 words
A test means according to Claim 1 or 2, characterized in that the reporter substance is a compound having the structure in which R I is H or lower alkyl, R * is H or intermediate alkyl and X is a halogen or pseudohalogen.
Claim 6
Dependent Claim
18 words
A test means according to Claim 4, characterized in that R' is methyl and R * is n-decyl.
Claim 7
Dependent Claim
48 words
A test means according to any of Claims 1 to 5, characterized in that the reporter substance is one capable of producing the appearance of, or change in, fluorescence in the presence of the complex of the ionophore and ion, and preferably is fluorescein or a derivative thereof.
Claim 8
Dependent Claim
47 words
A test means according to any of Claims 1 to 5, characterized in that the reporter substance comprises at least two precursor substances which are capable of participating in the formation of a detectable response in the presence of the complex of the ionophore and the ion.
Claim 9
Dependent Claim
55 words
A test means according to Claim 7, characterized in that the precursor substances have the respective structure and in which X is halogen or a pseudohalogen, R is a 2-, 3-, 5-, and/or 6-position substituent selected from lower or intermediate alkyl, aryl and a fused ring at the 2,3- or 5,6-positions, and n is 0-4.
Claim 10
Dependent Claim
18 words
test means according to Claim 7, characterized in that the precursor substances are starch and an oxidizing agent.
Claim 11
Dependent Claim
49 words
A test device for determining the presence of an ion in a test sample, the test device comprising an elongated support member having an upper substantially flat face, and the test means of any one of Claims 1 - 9 affixed to the flat face of the support member.
Claim 12
Dependent Claim
45 words
A method for determining the presence of an ion in an aqueous test sample, the method comprising contacting the test sample with the test means of any one of Claims 1 - 9 or the test device of Claim 10 and observing a detectable response.
References Cited (Backward Citations)
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