US4670218A
Expired / Lapsed
US
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Ion test means having a porous carrier matrix
Assignee: Bayer Corp
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Filing: 1985-06-24
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Granted: 1987-06-02
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Expires: 2005-06-24
Abstract
Abstract A test means for determining the presence of an ion in an aqueous test sample is disclosed, as well as a test device incorporating the test means, a method for preparing it and a method for using it. The test means is composed of a porous carrier matrix incorporated, substantially uniformly, with a homogeneous hydrophobic composition and with a buffering substance capable of providing a pH in the range of from about 5 to 10. The homogeneous hydrophobic composition contains an ionophore capable of forming a complex with the ion, a hydrophobic substance and a reporter substance capable of interacting with the complex of ionophore and ion to produce a detectable response. In a preferred embodiment, this invention provides a colorimetric test capable of measuring serum potassium.
Inventors
- 👤 Mary L. Gantzer
- 👤 Paul R. Hemmes, Jr.
- 👤 Daniel Wong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 11:13
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/84
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
118 words
A test means for determining the presence of an ion in an aqueous test sample, the test means comprising a carrier matrix having a porous structure substantially uniformly incorporated with (a) a homogeneous hydrophobic composition containing an ionophore capable of forming a complex with a specific ion to be determined, a reporter substance capable of interacting with the complex of the ionophore and the ion to produce a detectable response and a hydrophobic substance; and (b) a buffering substance capable of providing a pH in the range of from about 5 to 10; the porous structure of the matrix being maintained after the incorporation and drying wherein an aqueous sample flows readily into the carrier matrix upon contact.
Claim 2
Dependent Claim
118 words
A test means for determining the presence of an ion in an aqueous test sample, the test means comprising a carrier matrix having a porous structure substantially uniformly incorporated with (a) a homogeneous hydrophobic composition containing an ionophore capable of forming a complex with a specific ion to be determined, a reporter substance capable of interacting with the complex of the ionophore and the ion to produce a detectable response and a hydrophobic substance; and (b) a buffering substance capable of providing a pH in the range of from about 5 to 10; the porous structure of the matrix being maintained after the incorporation and drying wherein an aqueous sample flows readily into the carrier matrix upon contact.
Claim 3
Dependent Claim
27 words
The test means of claim 1 in which the porous carrier matrix is additionally incorporated with an interferant removal substance capable of interacting with an interfering ion.
Claim 4
Dependent Claim
19 words
The test means of claim 1 in which the porous carrier matrix is additionally incorporated with a wetting substance.
Claim 5
Dependent Claim
14 words
The test means of claim 1 in which the porous carrier matrix is paper.
Claim 6
Dependent Claim
131 words
A test means for determining the presence of a cation in an aqueous test sample, the test means comprising a carrier matrix having a porous structure substantially uniformly incorporated with (a) a homogeneous hydrophobic composition containing an ionophore capable of forming a complex with a cation to be determined, a neutral reporter substance having a dissociable proton which proton is capable of dissociating upon interaction of the reporter with the complex of the ionophore and the cation to produce a detectable response and a hydrophobic substance; and (b) a buffering substance capable of providing a pH in the range of from about 5 to 10; the porous structure of the matrix being maintained after the incorporation and drying wherein an aqueous test sample flows readily into the carrier matrix upon contact.
Claim 7
Dependent Claim
131 words
A test means for determining the presence of a cation in an aqueous test sample, the test means comprising a carrier matrix having a porous structure substantially uniformly incorporated with (a) a homogeneous hydrophobic composition containing an ionophore capable of forming a complex with a cation to be determined, a neutral reporter substance having a dissociable proton which proton is capable of dissociating upon interaction of the reporter with the complex of the ionophore and the cation to produce a detectable response and a hydrophobic substance; and (b) a buffering substance capable of providing a pH in the range of from about 5 to 10; the porous structure of the matrix being maintained after the incorporation and drying wherein an aqueous test sample flows readily into the carrier matrix upon contact.
Claim 8
Dependent Claim
35 words
The test means of claim 5 in which the reporter substance is one capable of producing the appearance of, or change in, fluorescence in the presence of the complex of the ionophore and the ion.
Claim 9
Dependent Claim
17 words
The test means of claim 6 in which the reporter substance is fluorescein or a derivative thereof.
Claim 10
Dependent Claim
27 words
The test means of claim 1 in which the specified cation to be determined is sodium ion or calcium ion and the porous carrier matrix is paper.
Claim 11
Dependent Claim
14 words
The test means of claim 8 in which the ionophore is an uncharged podand.
Claim 12
Dependent Claim
35 words
The test means of claim 5 in which the reporter substance is one capable of producing the appearance of, or change in, color in the presence of the complex of the ionophore and the cation.
Claim 13
Dependent Claim
70 words
The test means of claim 10 in which the reporter substance is a compound having the structure ##STR14## in which X, same or different, is a halogen or pseudohalogen; in which each R, same or different, is a 2-, 3-, 5-, or 6-position substituent, or multiple substituents thereof, selected from lower alkyl, intermediate alkyl, aryl or a fused ring at the 2,3- or 5,6-positions; and n is 0 to 4.
Claim 14
Dependent Claim
41 words
The test means of claim 10 in which the reporter substance is a compound having the structure ##STR15## in which R' is H or lower alkyl, R* is H or intermediate alkyl and X, same or different, is halogen or pseudohalogen.
Claim 15
Dependent Claim
15 words
The test means of claim 12 in which R' is methyl and R* is n-decyl.
Claim 16
Dependent Claim
31 words
The test means of claim 13 in which the specific cation to be determined is potassium ion, the porous carrier matrix is paper and the ionophore is an uncharged crown ether.
Claim 17
Dependent Claim
14 words
The test means of claim 14 in which the uncharged crown ether is 2,3-naphtho-1,4,7,10,13-pentaoxacyclopentadeca-2-ene.
Claim 18
Dependent Claim
15 words
The test means of claim 15 in which the hydrophobic substance is styrene/maleic anhydride copolymer.
Claim 19
Dependent Claim
129 words
A test means for determining the presence of an ion in an aqueous test sample, the test means comprising a porous, fibrous carrier matrix having an open lattice structure substantially uniformly incorporated with (a) a homogeneous hydrophobic composition containing: (i) an ionophore capable of forming a complex with a specific ion to be determined; (ii) a reporter substance capable of interacting with the complex of the ionophore and the ion to produce a detectable response; and (iii) a hydrophobic substance; and (b) a buffering substance capable of providing a pH in the range of from 5 to 10; wherein the open lattice structure of the fibrous matrix is maintained after incorporation with the hydrophobic composition and buffering substance, allowing a sample to flow readily into the matrix upon contact.
Claim 20
Dependent Claim
129 words
A test means for determining the presence of an ion in an aqueous test sample, the test means comprising a porous, fibrous carrier matrix having an open lattice structure substantially uniformly incorporated with (a) a homogeneous hydrophobic composition containing: (i) an ionophore capable of forming a complex with a specific ion to be determined; (ii) a reporter substance capable of interacting with the complex of the ionophore and the ion to produce a detectable response; and (iii) a hydrophobic substance; and (b) a buffering substance capable of providing a pH in the range of from 5 to 10; wherein the open lattice structure of the fibrous matrix is maintained after incorporation with the hydrophobic composition and buffering substance, allowing a sample to flow readily into the matrix upon contact.
Claim 21
Dependent Claim
43 words
The test means of claim 17 in which the reporter is a neutral compound having a dissociable proton which proton is capable of dissociating upon interaction of the reporter with the complex of the ionophore and the ion to produce the detectable response.
Claim 22
Independent Claim
199 words
A method for preparing a test means for determining the presence of an ion in an aqueous test sample, the method comprising the steps of (a) forming a homogeneous first mixture of the hydrophobic composition comprising an ionophore capable of forming a complex with a specific ion to be determined, a reporter substance capable of interacting with the complex of the ionophore and the ion to produce a detectable response, a hydrophobic substance and an organic solvent; and (b) forming a homogeneous second mixture of a buffering substance capable of providing a pH in the range of from about 5 to 10 and water or a water-miscible solvent or mixtures thereof; (c) substantially uniformily incorporating a carrier matrix having a porous structure with one of the first or the second mixtures; (d) drying the incorporated matrix; (e)substantially uniformly incorporating the porous carrier matrix with the other of the first or second mixtures to form a doubly incorporated matrix; and (f) drying the doubly incorporated carrier matrix; wherein the porous structure of the matrix is maintained after incorporation of the hydrophobic composition and buffering substacne and drying so that an aqueous test sample flows readily into the matrix upon contact.
Claim 23
Independent Claim
199 words
A method for preparing a test means for determining the presence of an ion in an aqueous test sample, the method comprising the steps of (a) forming a homogeneous first mixture of the hydrophobic composition comprising an ionophore capable of forming a complex with a specific ion to be determined, a reporter substance capable of interacting with the complex of the ionophore and the ion to produce a detectable response, a hydrophobic substance and an organic solvent; and (b) forming a homogeneous second mixture of a buffering substance capable of providing a pH in the range of from about 5 to 10 and water or a water-miscible solvent or mixtures thereof; (c) substantially uniformily incorporating a carrier matrix having a porous structure with one of the first or the second mixtures; (d) drying the incorporated matrix; (e)substantially uniformly incorporating the porous carrier matrix with the other of the first or second mixtures to form a doubly incorporated matrix; and (f) drying the doubly incorporated carrier matrix; wherein the porous structure of the matrix is maintained after incorporation of the hydrophobic composition and buffering substacne and drying so that an aqueous test sample flows readily into the matrix upon contact.
Claim 24
Dependent Claim
23 words
The method of claim 19 in which the second mixture additionally contains an interferant removal substance capable of interacting with an interfering ion.
Claim 25
Dependent Claim
15 words
The method of claim 19 in which the second mixture additionally contains a wetting substance.
Claim 26
Dependent Claim
13 words
The method of claim 19 in which the porous carrier matrix is paper.
Claim 27
Dependent Claim
51 words
The method of claim 19 in which the specific ion to be determined is a cation and the reporter is a neutral compound having a dissociable proton which proton is capable of dissociating upon interaction of the reporter with the complex of the ionophore and the cation to produce detectable response.
References Cited (Backward Citations)
US3630957A
Cited
US3616251A
Cited
US3635679A
Cited
US3992158A
Cited
US4061468A
Cited
US4367072A
Cited
DE2842862A1
Cited
US4272485A
Cited
US4272484A
Cited
US4356149A
Cited
EP0041175A2
Cited
US4540520A
Cited
US4552697A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-01 | 1990-12-01 | 1991-06-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-01 | 1994-12-01 | 1995-06-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-01 | 1998-12-01 | 1999-06-02 | $7700.00 | Window Closed / Lapsed |